Pathotypic characterization of Newcastle disease virus isolated from vaccinated chicken in West Java, Indonesia

Vet World Vol.10 April-2017 Article-11

Research Article

Veterinary World, 10(4): 438-444

https://doi.org/10.14202/vetworld.2017.438-444

Dwi Desmiyeni Putri^1,2, Ekowati Handharyani³, Retno Damajanti Soejoedono⁴, Agus Setiyono³, Ni Luh Putu Ika Mayasari⁴, and Okti Nadia Poetri⁴

1. Study Program of Animal Biomedical Science, IPB Graduate School, Bogor Agricultural University, Bogor, Indonesia.
2. Study Program of Animal Husbandry, Department of Animal Husbandry, State Polytechnic of Lampung, Lampung, Indonesia.
3. Department of Veterinary Clinic Reproduction and Pathology, Faculty of Veterinary Medicine, Bogor Agricultural University, Bogor, Indonesia.
4. Department of Animal Diseases and Veterinary Public Health, Faculty of Veterinary Medicine, Bogor Agricultural University, Bogor, Indonesia.

Background and Aim: This research was conducted to differentiate and characterize eight Newcastle disease virus (NDV) isolates collected from vaccinated chicken at commercial flocks in West Java, Indonesia, in 2011, 2014 and 2015 by pathotype specific primers.

Materials and Methods: A total of eight NDV isolates collected from clinical outbreaks among commercial vaccinated flocks in West Java, Indonesia, in 2011, 2014, and 2015 were used in this study. Reverse transcription-polymerase chain reaction was used to detect and differentiate virulence of NDV strains, using three sets of primers targeting their M and F gene. First primers were universal primers to detect NDV targeting matrix (M) gene. Other two sets of primers were specific for the fusion (F) gene cleavage site sequence of virulent and avirulent NDV strains.

Results: Our results showed that three isolates belong to NDV virulent strains, and other five isolates belong to NDV avirulent strains. The nucleotide sequence of the F protein cleavage site showed 112K/R-R-Q/R-K-R/G-F117 on NDV virulent strains and 112G-K/R-Q-G-R-L117 on NDV avirulent strain.

Conclusion: Result from the current study suggested that NDV virulent strain were circulating among vaccinated chickens in West Java, Indonesia; this might possess a risk of causing ND outbreaks and causing economic losses within the poultry industry. Keywords: F gene, M gene, molecular pathotyping, Newcastle disease virus, vaccinated chickens, virulence.

Keywords: F gene, M gene, molecular pathotyping, Newcastle disease virus, vaccinated chickens, virulence.

How to cite this article: Putri DD, Handharyani E, Soejoedono RD, Setiyono A, Mayasari NI, Poetri ON (2017) Pathotypic characterization of Newcastle disease virus isolated from vaccinated chicken in West Java, Indonesia, Veterinary World, 10(4): 438-444.

Received: 21-10-2016 Accepted: 09-03-2017 Published online: 22-04-2017

Corresponding author: Dwi Desmiyeni Putri E-mail: desmiyenidwi@polinela.ac.id

DOI: 10.14202/vetworld.2017.438-444

Copyright: Putri, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.