Vet World   Vol.10   April-2017  Article-14

Exploring the potential hazard of Mycobacterium avium subspecies paratuberculosis as a cause for Crohn's disease

Background and Aim: The present study was aimed to detect MAP in milk by polymerase chain reaction (PCR) targeting IS900 and to describe the excretion pattern of MAP in milk from asymptomatic lactating cows and does with relevance to the public health significance.

Materials and Methods: A total of 77 milk samples were collected randomly from lactating animals which include cows (45) and does (32). All the 77 milk samples were processed to identify the presence of MAP by employing the direct IS900 PCR as per the standard protocol.

Results: Out of 77 milk samples from asymptomatic lactating animals, 12 (15.58%) were showed positivity for IS900 PCR in which 5 (11.11%) were from lactating cows and 7 (21.87%) were from lactating does.

Conclusion: In our study, 15.58% of milk samples showed IS900 positivity which indicates the presence of subclinical MAP infection in lactating animals. Hence, there is a possibility for excretion of MAP through milk which can be a potential threat for CD in humans by raw milk consumption. Therefore, the prevention of MAP in the food chain need to be assured by sourcing raw products from animal herds free of MAP infection. Keywords: Crohn's disease, excretion, food chain, IS900 polymerase chain reaction, Mycobacterium avium subspecies paratuberculosis, raw milk.

Keywords: Crohn's disease, excretion, food chain, IS900 polymerase chain reaction, Mycobacterium avium subspecies paratuberculosis, raw milk.

How to cite this article: Bharathy S, Gunaseelan L, Porteen K (2017) Exploring the potential hazard of Mycobacterium avium subspecies paratuberculosis as a cause for Crohn's disease, Veterinary World, 10(4): 457-460.

Received: 06-12-2016  Accepted: 02-03-2017     Published online: 28-04-2017

Corresponding author: Sukumar Bharathy   E-mail: bharathy.vet@gmail.com

DOI: 10.14202/vetworld.2017.457-460

Copyright: Bharathy, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.