Toxoplasma gondii Type I, predominant genotype isolated from sheep in South of Iran

Vet World Vol.10 April-2017 Article-4

Research Article

Veterinary World, 10(4): 386-392

https://doi.org/10.14202/vetworld.2017.386-392

Belal Armand¹, Kavous Solhjoo^1,2, Manoochehr Shabani Kordshooli², Mohammad Hasan Davami¹, Morteza Pourahmad³, and Vahideh Orfaee⁴

1. Department of Parasitology and Mycology, Jahrom University of Medical Sciences, Jahrom, Iran.
2. Department of Parasitic Disease, Zoonoses Research Center, Jahrom University of Medical Sciences, Jahrom, Iran.
3. Infectious Diseases and Tropical Medicine Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.
4. Department of Biology, Basic Sciences Faculty, Islamic Azad University, Jahrom Branch, Jahrom, Iran.

Background and Aim: This study was performed to determine the genetic diversity of Toxoplasma gondii in sheep using nested-polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in Southern Iran.

Materials and Methods: The tissue samples of diaphragm and heart from 125 sheep were collected from the main slaughterhouses of Jahrom district in South of Fars province, Iran, between Aprils and June 2013. The DNA were extracted and analyzed by nested-PCR using specific primers for SAG2 and GRA6 loci. RFLP was used to classify strains into one of the three major lineages of T. gondii.

Results: T. gondii Type I was predominant in this area. The data obtained from both loci demonstrated that the frequency of each genotype was 72% Type I, 2.4% Type III, 7.2% mixed Type I and II, 16.8% mixed Type I and III, 0.8% mixed Type II and III, and 0.8% mixed Type I, II and III.

Conclusion: Although the previously published data indicated that Type II is the predominant T. gondii genotype in sheep in the other parts of the world, this study showed that genotype I is the dominant genotype of T. gondii in the southern Iran; however, other genotypes were detected. High diversity of T. gondii genotypes including mix genotypes in lambs is of importance for the public health. These studies depict a new mapping of T. gondii genotypes pattern which could be very helpful in toxoplasmosis control and prevention. Keywords: genotyping, GRA6 gene, Iran, polymerase chain reaction-restriction fragment length polymorphism, SAG2 gene, sheep, Toxoplasma gondii.

Keywords: genotyping, GRA6 gene, Iran, polymerase chain reaction-restriction fragment length polymorphism, SAG2 gene, sheep, Toxoplasma gondii.

How to cite this article: Armand B, Solhjoo K, Kordshooli MS, Davami MH, Pourahmad M, Orfaee V (2017) Toxoplasma gondii Type I, predominant genotype isolated from sheep in South of Iran, Veterinary World, 10(4): 386-392.

Received: 29-10-2016 Accepted: 23-02-2017 Published online: 07-04-2017

Corresponding author: Kavous Solhjoo E-mail: solhjouk@yahoo.com

DOI: 10.14202/vetworld.2017.386-392

Copyright: Armand, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.