Open Access
Research (Published online: 07-04-2017)
4. Toxoplasma gondii Type I, predominant genotype isolated from sheep in South of Iran
Belal Armand, Kavous Solhjoo, Manoochehr Shabani Kordshooli, Mohammad Hasan Davami, Morteza Pourahmad and Vahideh Orfaee
Veterinary World, 10(4): 386-392

Belal Armand: Department of Parasitology and Mycology, Jahrom University of Medical Sciences, Jahrom, Iran.
Kavous Solhjoo: Department of Parasitology and Mycology, Jahrom University of Medical Sciences, Jahrom, Iran; Department of Parasitic Disease, Zoonoses Research Center, Jahrom University of Medical Sciences, Jahrom, Iran.
Manoochehr Shabani Kordshooli: Department of Parasitic Disease, Zoonoses Research Center, Jahrom University of Medical Sciences, Jahrom, Iran.
Mohammad Hasan Davami: Department of Parasitology and Mycology, Jahrom University of Medical Sciences, Jahrom, Iran.
Morteza Pourahmad: Infectious Diseases and Tropical Medicine Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.
Vahideh Orfaee: Department of Biology, Basic Sciences Faculty, Islamic Azad University, Jahrom Branch, Jahrom, Iran.

doi: 10.14202/vetworld.2017.386-392

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Article history: Received: 29-10-2016, Accepted: 23-02-2017, Published online: 07-04-2017

Corresponding author: Kavous Solhjoo


Citation: Armand B, Solhjoo K, Kordshooli MS, Davami MH, Pourahmad M, Orfaee V (2017) Toxoplasma gondii Type I, predominant genotype isolated from sheep in South of Iran, Veterinary World, 10(4): 386-392.

Aim: This study was performed to determine the genetic diversity of Toxoplasma gondii in sheep using nested-polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in Southern Iran.

Materials and Methods: The tissue samples of diaphragm and heart from 125 sheep were collected from the main slaughterhouses of Jahrom district in South of Fars province, Iran, between Aprils and June 2013. The DNA were extracted and analyzed by nested-PCR using specific primers for SAG2 and GRA6 loci. RFLP was used to classify strains into one of the three major lineages of T. gondii.

Results: T. gondii Type I was predominant in this area. The data obtained from both loci demonstrated that the frequency of each genotype was 72% Type I, 2.4% Type III, 7.2% mixed Type I and II, 16.8% mixed Type I and III, 0.8% mixed Type II and III, and 0.8% mixed Type I, II and III.

Conclusion: Although the previously published data indicated that Type II is the predominant T. gondii genotype in sheep in the other parts of the world, this study showed that genotype I is the dominant genotype of T. gondii in the southern Iran; however, other genotypes were detected. High diversity of T. gondii genotypes including mix genotypes in lambs is of importance for the public health. These studies depict a new mapping of T. gondii genotypes pattern which could be very helpful in toxoplasmosis control and prevention.

Keywords: genotyping, GRA6 gene, Iran, polymerase chain reaction-restriction fragment length polymorphism, SAG2 gene, sheep, Toxoplasma gondii.


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