Article history: Received: 04-12-2016, Accepted: 27-02-2017, Published online: 10-04-2017
Corresponding author: God'spower Richard Okoh
E-mail: email@example.comCitation: Mailafia S, Okoh GR, Olabode HOK, Osanupin R (2017) Isolation and identification of fungi associated with spoilt fruits vended in Gwagwalada market, Abuja, Nigeria, Veterinary World, 10(4): 393-397.
Aim: Annual reports have shown that 20% of fruits and vegetables produced are lost to spoilage. This study was undertaken to isolate and identify fungi that are associated with spoilt fruits commonly sold in Gwagwalada market, Abuja, and recommend appropriate control measure.
Materials and Methods: The study was conducted in Gwagwalada metropolis, Gwagwalada Area Council of the Federal Capital Territory, Abuja, Nigeria. A total of 100 spoilt fruits which include pawpaw (Carica papaya), orange (Citrus sinensis), tomato (Lycopersicon esculentum), pineapple (Ananas comosus), and watermelon (Citrullus vulgaris) were purchased and examined for the presence of fungal organisms using standard methods. The data collected were analyzed using simple descriptive statistics (frequency and mean) and analysis of variance (p<0.05).
Results: Aspergillus niger had the highest occurrence in pineapple, watermelon, oranges, pawpaw, and tomatoes with a frequency of 38%. Fusarium avenaceum followed with the frequency of occurrence of 31% in fruits such as pineapple, watermelon, oranges, pawpaw, and tomatoes while Penicillium digitatum and Rhizopus stolonifer had the least frequency of 4% each in tomato; and orange and tomato, respectively. Other fungal species were identified as yeast (Saccharomyces species) (10%), Fusarium solani (8%), and Aspergillus flavus (5%). The highest prevalence rate was 70% of A. niger from orange followed by F. avenaceum of which 65% isolates were recovered from pawpaw. Other fungal organisms such as yeast (Saccharomyces species), P. digitatum and R. stolonifer were isolated with varying prevalence (40%, 20%, and 5%) from watermelon, tomato, and orange, respectively. However, there was no significant difference in the fungal load of the various fruits studied (analysis of variance=478.2857, p<0.05, F=4.680067 and df=34).
Conclusion: The pathogenic fungi species associated with fruits spoilage in this study are of economical and public health significance. A. niger causes black mold in certain fruits and vegetables. Some strains of A. niger have been reported to produce potent mycotoxins called ochratoxins that can be harmful to human beings and animals. Care should be taken during handling of these fruits and improved technology based preservation methods are suggested to enhance the keeping quality of fruits.
Keywords: frequency of occurrence, fruits, fungi, pathogenic, prevalence.
1. Ikhiwili, O.M. (2012) Isolation and Characterisation of Microorganisms Associated with Rot Diseases of Fruit, Stem and Leaf of Carica papaya L. A Project Report Submitted to the Department of Biological Sciences, College of Science and Technology, Covenant University, Canaanland, Ota, Ogun state, Nigeria. p5-6.
2. Al-Hindi, R.R., Al-Najada, A.R. and Mohamed, S.A. (2011) Isolation and identification of some fruit spoilage fungi: Screening of plant cell wall degrading enzymes. Afr. J. Microbiol. Res., 5(4): 443-448.
3. Lewis, R.A. (2002) CRC Dictionary of Agricultural Sciences. CRC Press, Boca Raton, FL.
4. Singh, D. and Sharma, R.R. (2007) Postharvest diseases of fruit and vegetables and their management. In: Prasad, D., editor. Sustainable Pest Management. Daya Publishing House, New Delhi, India.
5. Droby, S. (2006) Improving quality and safety of fresh fruits and vegetables after harvest by the use of biocontrol agents and natural materials. Acta Hortic., 709: 45-51. [Crossref]
6. Barth, M., Hankison, T.R, Zhuang, H. and Breidt, F. (2009) Microbiological spoilage of fruits and vegetables. Sperber, W.H. and Doyle, M.P., editors. Compendium of the Microbiological Spoilage of Foods and Beverages, Food Microbiology and Food Safety. C Springer Science Business Media, LLC, New York. p135-183. [Crossref]
7. Thiyam, B. and Sharma, G.D. (2013) Isolation and identification of fungi associated with local fruits of Barak Valley, Assam. Curr. World Environ., 8(2): 319-322. [Crossref]
8. Zhu, S.J. (2006) Non-chemical approaches to decay control in postharvest fruit. In: Noureddine, B. and Norio, S., editors. Advances in Postharvest Technologies for Horticultural Crops. Research Signpost, Trivandrum, India. p297-313.
10. Afsah-Hejri, L., Jinap, S., Hajeb, P., Radu, S. and Shakibazadeh, S. (2013) A review on mycotoxins in food and feed: Malaysia case study. Compr. Rev. Food Sci. Food Saf., 12(6): 629-651. [Crossref]
11. Petzinger, E. and Weidenbach, A. (2002) Mycotoxins in the food chain: The role of ochratoxins. Livest. Prod. Sci., 76: 245-250. [Crossref]
12. Anon. (2009) Annual Report, Ministry of Livestock Development and Fisheries. Dar-es-Salaam, Tanzania, Mainland.
13. Awowole, B.F. (2007) This is a Waste Daily Sun. The Sun Publishing Limited, Lagos. p10-23.
14. Tafinta, I.Y., Shehu, K., Abdulganiyyu, H., Rabe, A.M. and Usman, A. (2013) Isolation and identification of fungi associated with the spoilage of sweet orange (Citrus sinensis) fruits in Sokoto State. Niger. J. Basic Appl. Sci., 21(3): 193-196.
15. Oyeleke, A. and Manga, S.B. (2008) Essential of Laboratory Practice. 3rd ed. Tobest Publisher, Minna, Niger state, Nigeria. p12-29.
16. Adebayo-Tayo, B.C., Odu, N., Esen, C.U. and Okonko, T.O. (2012) Microorganisms associated with spoilage of stored vegetables in Uyo metropolis, Akwa Ibom state, Nigeria. Nat. Sci., 10(3): 23-32.
17. Onuorah, S., Obika, I. and Okafor, U. (2015) Filamentous fungi associated the spoilage of post-harvest sweet orange fruits (Citrus sinensis) sold in Awka Major Markets, Nigeria. Bioeng. Biosci., 3(3): 44-49.
18. Klich, M.A. (2002) Identification of Common Aspergillus Species. Centraalbureau Voor Schimmelautures, Netherlands.
19. Samson, R.A. and Varga, J. (2007) Aspergillus Systematics in the Genomic Era. CBS Fungal Biodiversity Centre, Utrecht. p206.
20. Baiyewu, R.A., Amusa, N.A., Ayoola, O.A. and Babalola, O.O. (2007) Survey of the postharvest diseases and aflatoxin contamination of marketed Pawpaw fruit (Carica papaya L.) in South Western Nigeria. Afr. J. Agric. Res., 2(4): 178-181.
21. Chukwuka, K.S., Okonko, I.O. and Adekunle, A.A. (2010) Microbial ecology of organisms causing pawpaw (Carica papaya L.) fruit decay in Oyo State, Nigeria. Am. Eurasian J. Toxicol. Sci., 2(1): 43-50.
22. Gultie, A., Sahile, S. and Subramanian, C. (2013) Assessment of fruit management in Gondar town markets of North Western Ethiopia. GJBAHS, 2(4): 4-8.
23. Okojie, P.W. and Isah, E.C. (2014) Sanitary conditions of food vending sites and food handling practices of street food vendors in Benin city, Nigeria: Implication for food hygiene and safety. J. Environ. Public Health, 2014: 1-6. [Crossref]
24. Pawlowska, A.M., Zannini, E., Coffey, A. and Arendt, E.K. (2012) Green preservatives: Combating fungi in the food and feed industry by applying antifungal lactic acid bacteria. Adv. Food Nutr. Res. 66: 217-238. [Crossref]
25. Sorensen, J.L., Phipps, R.K., Nielsen, K.F., Schroers, H.J., Frank, J. and Thrane, U. (2009) Analysis of Fusarium avenaceum metabolites produced during wet apple core rot. J. Agric. Food Chem., 57: 1632-1639. [Crossref] [PubMed]
26. Lysoe, E., Harris, L.J., Walkowiak, S., Subramaniam, R., Divon, H.H., Riiser, E.S., Llorens, C., Gabaldon, T., Kistler, H.C., Jonkers, W., Kolseth, A.K., Nielsen, K.F., Thrane, U. and Frandsen, R.J.N. (2014) The genome of the generalist plant pathogen Fusarium avenaceum is enriched with genes involved in redox, signaling and secondary metabolism. Plos One, 9(11): e112703. [Crossref]
27. Varvas, T., Kasekamp, K. and Kullman, B. (2013) Preliminary study of endophytic fungi in timothy (Phleum pratense) in Estonia. Acta Mycol., 48: 41-49. [Crossref]
28. Yacoub, A. (2012) The first report on entomopathogenic effect of Fusarium avenaceum (fries) Saccardo (Hypocreales, Ascomycota) against rice weevil (Sitophilus oryzae L: Curculionidae, Coleoptera). J. Entomol. Acarol. Res., 44: 51-55.
29. Oshikata, C., Tsurikisawa, N., Saito, A., Watanabe, M., Kamata, Y., Tanaka, M., Tsuburai, T., Mitomi, H., Takatori, K., Yasueda, H. and Akiyama, K. (2013) Fatal pneumonia caused by Penicillium digitatum: A case report. BMC Pulm. Med., 13: 16. [Crossref] [PubMed] [PMC]
30. Foody, E. and Tong, C. (2008) An Informative, Heart-Warming Tale About Black Bread Mold. Classroom Project: Organism Research and Creative Story Telling Ashbury College School Ottawa, Ontario K1M 0T3 Canada.
31. Gadgile, D.P. and Chavan, A.M. (2010) Impact of temperature and relative humidity on development of Aspergillus flavus rot of mango fruit. Sci. Technol., 3: 48-49.
32. Bali, R.V., Bindu, M.G., Chenga, R.V. and Reddy, K. (2008) Post harvest fungal spoilage in sweet orange (Citrus sinensis) and acid lime (Citrus aurentifolia Swingla) at different stages of marketing. Agric. Sci. Digest, 28: 265-267.
33. Okereke, V.C., Godwin-Egein, M.I. and Arinze, A.E. (2010) Assessment of postharvest rot of mango at different stages of market in Port Harcourt, Nigeria. Int. J. Curr. Res., 11: 6-10.
34. Boyer, R. (2009) Using dehydration to preserve fruits, vegetables, and meats. Issued in furtherance of cooperative extension work. Virginia Polytechnic Institute and State University, Virginia State University, and the U.S. Department of Agriculture Cooperating. Edwin J. Jones, Director, Virginia Cooperative Extension, Virginia Tech, Blacksburg; M. Ray McKinnie, Interim Administrator, 1890 Extension Program, Virginia State University, Petersburg. p348-597.35. Msagati, T. (2012) The Chemistry of Food Additives and Preservatives. Blackwell Publishing Ltd., Oxford, UK. [Crossref]