Open Access
Research (Published online: 01-08-2017)
1. Detection of Staphylococcus aureus enterotoxigenic strains in bovine raw milk by reversed passive latex agglutination and multiplex polymerase chain reaction
Asmaa Samy Mansour, Gad El-Said Wagih, Sabry D. Morgan, Mahmoud Elhariri, Mona A. El-Shabrawy, Azza S. M. Abuelnaga and E. A. Elgabry
Veterinary World, 10(8): 843-847

Asmaa Samy Mansour: Department of Microbiology and Immunology, National Research Centre, Dokki, Giza, Egypt.
Gad El-Said Wagih: Department of Microbiology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.
Sabry D. Morgan: Department of Milk Hygiene and Control, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.
Mahmoud Elhariri: Department of Microbiology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.
Mona A. El-Shabrawy: Department of Microbiology and Immunology, National Research Centre, Dokki, Giza, Egypt.
Azza S. M. Abuelnaga: Department of Microbiology and Immunology, National Research Centre, Dokki, Giza, Egypt.
E. A. Elgabry: Department of Microbiology and Immunology, National Research Centre, Dokki, Giza, Egypt.

doi: 10.14202/vetworld.2017.843-847

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Article history: Received: 12-02-2017, Accepted: 21-06-2017, Published online: 01-08-2017

Corresponding author: Mahmoud Elhariri


Citation: Mansour AS, Wagih GE, Morgan SD, Elhariri M, El-Shabrawy MA, Abuelnaga ASM, Elgabry EA (2017) Detection of Staphylococcus aureus enterotoxigenic strains in bovine raw milk by reversed passive latex agglutination and multiplex polymerase chain response, Veterinary World, 10(8): 843-847.

Aim: This study gives an outline of the assessment of enterotoxigenic Staphylococcus aureus tainting levels in raw milk from different sources in Egypt and characterization of enterotoxigenic strains utilizing a technique in light of PCR to identify genes coding for the production of staphylococcal enterotoxin (SE). The obtained data were compared with results from the application of the reversed passive latex.

Materials and Methods: Multiplex PCR and reversed passive latex agglutination (RPLA) were used. A total of 141 samples of raw milk (cow's milk=33, buffalo's milk=58, and bulk tank milk=50) were investigated for S. aureus contamination and tested for enterotoxin genes presence and toxin production.

Results: S. aureus was detected in 23 (16.3%) samples phenotypically and genotypically by amplification of nuc gene. The S. aureus isolates were investigated for SEs genes (sea to see) by multiplex PCR and the toxin production by these isolates was screened by RPLA. SEs genes were detected in six isolates (26.1%) molecularly; see was the most observed gene where detected in all isolates, two isolates harbored seb, and two isolates harbored sec. According to RPLA, three isolates produced SEB and SEC.

Conclusion: This study revealed the widespread of S. aureus strains caring genes coding for toxins. The real significance of the presence of these strains or its toxins in raw milk and their possible impact a potential hazard for staphylococcal food poisoning by raw milk consumption. Therefore, detection of enterotoxigenic S. aureus strains in raw milk is necessary for consumer safety.

Keywords: enterotoxin genes, multiplex polymerase chain reaction, reversed passive latex agglutination, raw milk, S. aureus.


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