Detection of Staphylococcus aureus enterotoxigenic strains in bovine raw milk by reversed passive latex agglutination and multiplex polymerase chain reaction

Vet World Vol.10 August-2017 Article-1

Research Article

Veterinary World, 10(8): 843-847

https://doi.org/10.14202/vetworld.2017.843-847

Asmaa Samy Mansour¹, Gad El-Said Wagih², Sabry D. Morgan³, Mahmoud Elhariri², Mona A. El-Shabrawy¹, Azza S. M. Abuelnaga¹, and E. A. Elgabry¹

1. Department of Microbiology and Immunology, National Research Centre, Dokki, Giza, Egypt.
2. Department of Microbiology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.
3. Department of Milk Hygiene and Control, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.

Background and Aim: This study gives an outline of the assessment of enterotoxigenic Staphylococcus aureus tainting levels in raw milk from different sources in Egypt and characterization of enterotoxigenic strains utilizing a technique in light of PCR to identify genes coding for the production of staphylococcal enterotoxin (SE). The obtained data were compared with results from the application of the reversed passive latex.

Materials and Methods: Multiplex PCR and reversed passive latex agglutination (RPLA) were used. A total of 141 samples of raw milk (cow's milk=33, buffalo's milk=58, and bulk tank milk=50) were investigated for S. aureus contamination and tested for enterotoxin genes presence and toxin production.

Results: S. aureus was detected in 23 (16.3%) samples phenotypically and genotypically by amplification of nuc gene. The S. aureus isolates were investigated for SEs genes (sea to see) by multiplex PCR and the toxin production by these isolates was screened by RPLA. SEs genes were detected in six isolates (26.1%) molecularly; see was the most observed gene where detected in all isolates, two isolates harbored seb, and two isolates harbored sec. According to RPLA, three isolates produced SEB and SEC.

Conclusion: This study revealed the widespread of S. aureus strains caring genes coding for toxins. The real significance of the presence of these strains or its toxins in raw milk and their possible impact a potential hazard for staphylococcal food poisoning by raw milk consumption. Therefore, detection of enterotoxigenic S. aureus strains in raw milk is necessary for consumer safety. Keywords: enterotoxin genes, multiplex polymerase chain reaction, reversed passive latex agglutination, raw milk, S. aureus.

Keywords: enterotoxin genes, multiplex polymerase chain reaction, reversed passive latex agglutination, raw milk, S. aureus.

How to cite this article: Mansour AS, Wagih GE, Morgan SD, Elhariri M, El-Shabrawy MA, Abuelnaga ASM, Elgabry EA (2017) Detection of Staphylococcus aureus enterotoxigenic strains in bovine raw milk by reversed passive latex agglutination and multiplex polymerase chain response, Veterinary World, 10(8): 843-847.

Received: 12-02-2017 Accepted: 21-06-2017 Published online: 01-08-2017

Corresponding author: Mahmoud Elhariri E-mail: Mahmoud_Elhariri@cu.edu.eg

DOI: 10.14202/vetworld.2017.843-847

Copyright: Mansour, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.