Molecular screening for hemotropic mycoplasmas in captive Barbary sheep (Ammotragus lervia) in southern Brazil

Vet World Vol.10 August-2017 Article-15

Research Article

Veterinary World, 10(8): 924-926

https://doi.org/10.14202/vetworld.2017.924-926

Leonilda C. Santos^1,2, Odilon Vidotto³, Vivien M. Morikawa², Nelson J. R. Santos³, Thallitha S. W. J. Vieira², Ivan R. Barros Filho², Rafael F. C. Vieira², and Alexander W. Biondo²

1. Department of Sanitary Engineering, Engineering and Exact Sciences Center, Universidade Estadual do Oeste do Parana, Foz do Iguacu, PR 85870-650, Brazil.
2. Department of Veterinary Medicine, Universidade Federal do Parana, Curitiba, PR 80035-050, Brazil.
3. Department of Preventive Veterinary Medicine, Universidade Estadual de Londrina, Londrina, PR 86057-970, Brazil.

Background and Aim: This study is part of an active surveillance program for monitoring animal health status in endangered species, and was conducted to screen captive Barbary sheep (Ammotragus lervia) for hemoplasma infection.

Materials and Methods: A total of 12 blood samples were collected, DNA extracted and further tested by a pan-hemoplasma polymerase chain reaction protocol.

Results: Animals were clinically healthy and not infested by ectoparasites. Although housekeeping gene DNA was successfully amplified, all the Barbary sheep samples tested negative for Mycoplasma sp.

Conclusion: Notwithstanding the negative results, molecular pathogen surveys on Barbary sheep and other exotic wild mammals may provide insights regarding infection of endangered species caused by captivity stress in association with exposure to new pathogens worldwide. Keywords: aoudads, eperythrozoonosis, hemolytic anemia, hemoplasmas.

Keywords: aoudads, eperythrozoonosis, hemolytic anemia, hemoplasmas.

How to cite this article: Santos LC, Vidotto O, Morikawa VM, Santos NJR, Vieira TSWJ, Filho IRB, Vieira RFC, Biondo AW (2017) Molecular screening for hemotropic mycoplasmas in captive Barbary sheep (Ammotragus lervia) in southern Brazil, Veterinary World, 10(8): 924-926.

Received: 10-03-2017 Accepted: 06-07-2017 Published online: 15-08-2017

Corresponding author: Rafael F. C. Vieira E-mail: rvieira@ufpr.br

DOI: 10.14202/vetworld.2017.924-926

Copyright: Santos, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.