Preparation and evaluation of Salmonella Enteritidis antigen conjugated with nanogold for screening of poultry flocks

Vet World Vol.10 August-2017 Article-2

Research Article

Veterinary World, 10(8): 848-853

https://doi.org/10.14202/vetworld.2017.848-853

Hazem Mohammed Ibrahim¹, Rafik Hamed Sayed², Wafaa Ragab Abdel-Aziz¹, and Rafik Tawfik Soliman³

1. Department of Bacterial Sera and Antigen Research, Veterinary Serum and Vaccine Research Institute, Abbasia, Cairo, Egypt.
2. Central Laboratory for Evaluation of Veterinary Biologics, Abbasia, Cairo, Egypt.
3. Department of Microbiology, Faculty of Veterinary Medicine, Cairo University, Cairo, Egypt.

Background and Aim: The present work aimed to develop lateral flow immunochromatographic test (LFIT) for detection of Salmonella Enteritidis (SE) specific antibodies in chicken sera.

Materials and Methods: A rapid LFIT has been developed, in which SE Group D antigen labeled with the gold chloride molecules laid on the conjugate pad. Staphylococcus aureus protein A was used as capture antibody at the test line (T) of a nitrocellulose (NC) membrane and anti-SE antigen-specific rabbit antibodies were used as capture antibody at the control line (C) of the NC strip in the lateral flow layout device.

Results: Using the developed LFIT, the minimal amount of SE-specific antibodies that can be detected in chicken serum sample was 1427 enzyme-linked immunosorbent assay (ELISA) unit/100 μl that was equal to 0.1 μg (Ab)/100 μl sample. 100 suspected serum samples collected from a poultry flock were tested with the prepared SE-LFIT kits and the locally prepared stained Salmonella antigen, and the results were compared with those obtained from examination of these samples with Salmonella Group D antibody ELISA kit as the gold standard test. The sensitivity, specificity, and accuracy of the prepared SE-LFIT antigen kits were 94.4%, 90%, and 94%, respectively, while those obtained with stained Salmonella antigen were 88.8%, 90%, and 89%, respectively.

Conclusion: The developed test is a simple field rapid test of high sensitivity, specificity, and accuracy that can improve and facilitates rapid field surveillance of salmonellosis among chickens. Keywords: diagnosis, lateral flow, poultry, Salmonella Enteritidis.

Keywords: diagnosis, lateral flow, poultry, Salmonella Enteritidis.

How to cite this article: Ibrahim HM, Sayed RH, Abdel-Aziz WR, Soliman RT (2017) Preparation and evaluation of Salmonella Enteritidis antigen conjugated with nanogold for screening of poultry flocks, Veterinary World, 10(8): 848-853.

Received: 10-04-2017 Accepted: 22-06-2017 Published online: 02-08-2017

Corresponding author: Hazem Mohammed Ibrahim E-mail: dr.hazemibrahim@gmail.com

DOI: 10.14202/vetworld.2017.848-853

Copyright: Ibrahim, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.