Immunization of sheep against Echinococcus granulosus with protoscolex tegumental surface antigens

Vet World Vol.10 August-2017 Article-3

Research Article

Veterinary World, 10(8): 854-858

https://doi.org/10.14202/vetworld.2017.854-858

Manouchehr Valizadeh¹, Behzad Haghpanah², Alireza Badirzadeh¹, Elham Roointan¹, Shirzad Fallahi³, and Saber Raeghi⁴

1. Department of Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
2. Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
3. Department of Parasitology and Mycology, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran.
4. Department of Laboratory Sciences, Maragheh University of Medical Sciences, Maragheh, Iran.

Background and Aim: Cystic echinococcosis (CE) has potential economic effects to both animal products and human health. A vaccine to protect livestock against CE can be effective in reducing economic costs and increasing the livestock products. Protoscolex tegumental surface antigens (PSTSA) used to induce the production of specific antibodies against Echinococcus granulosus in sheep. The tegumental antigens were extracted from viable protoscolices by solubilization in sterile phosphate-buffered saline containing decanoyl-N-methylglucamine.

Materials and Methods: Ten lambs which were infected with CE (positive control), 10 negative control, and 10 test groups of sheep were included in the study. 300 μg emulsion of purified-PSTSA was injected intramuscularly in a two-step immunization on the first and 30 days. Sera were collected immediately before immunization and 6 times with 10-day intervals until 60 days post immunization. Thereafter, the sera were tested for antibodies by indirect hemagglutination test in microtiter plate.

Results: After two immunizations, all the infected animals in test group showed substantial increases in antibody titer. Statistical analysis showed a significant difference between the titer obtained in the test and negative control groups in both phases of immunization (p<0.05).

Conclusion: The results showed that the PSTSA is a promising immunogenic compound for immunization of sheep against CE. Keywords: cystic echinococcosis, Echinococcus granulosus, immunization, Iran, Protoscolex tegumental surface antigens, sheep.

Keywords: cystic echinococcosis, Echinococcus granulosus, immunization, Iran, Protoscolex tegumental surface antigens, sheep.

How to cite this article: Valizadeh M, Haghpanah B, Badirzadeh A, Roointan E, Fallahi S, Raeghi S (2017) Immunization of sheep against Echinococcus granulosus with protoscolex tegumental surface antigens, Veterinary World, 10(8): 854-858.

Received: 27-03-2017 Accepted: 22-06-2017 Published online: 03-08-2017

Corresponding author: Saber Raeghi E-mail: saberraeghi@gmail.com

DOI: 10.14202/vetworld.2017.854-858

Copyright: Valizadeh, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.