Isolation and identification of a cellulolytic Enterobacter from rumen of Aceh cattle

Vet World Vol.10 December-2017 Article-16

Research Article

Veterinary World, 10(12): 1515-1520

https://doi.org/10.14202/vetworld.2017.1515-1520

Wenny Novita Sari¹, Safika², Darmawi³, and Yudha Fahrimal⁴

1. Postgraduate student of Mathematics and Applied Sciences, Syiah Kuala University, Darussalam, Banda Aceh 23111, Indonesia.
2. Department of Veterinary Infectious Diseases and Veterinary Public Health, Faculty of Veterinary Medicine, Bogor Agricultural University, Jalan Agatis IPB, Darmaga, Bogor, Indonesia.
3. Microbiology Laboratory, Faculty of Veterinary Medicine, Syiah Kuala University, Darussalam, Banda Aceh 23111, Indonesia.
4. Parasitology Laboratory, Faculty of Veterinary, Syiah Kuala University, Darussalam, Banda Aceh 23111, Indonesia.

Background and Aim: The aim of this study was to isolate and identify a cellulolytic bacterium from the rumen fluid of Aceh's cattle. Biodegradation by cellulolytic rumen bacteria can be used as a source of cellulolytic bacteria that act to degrade feed fibrous material so as to improve the quality of nutrients and digestibility of feed ingredients at a cheaper price than the use of commercial cellulase enzymes.

Materials and Methods: Samples were collected from rumen fluid of Aceh's cattle in Abattoirs (RPH) of Banda Aceh city, Indonesia, isolation, and screening of cellulolytic bacteria were done in Microbiology Laboratory, Faculty of Veterinary Medicine, Syiah Kuala University, Banda Aceh, Indonesia.

Results: The S1 isolates showed ±2.5 cm of clear zone diameter. Microscopically, this strain was found to be a Gram-negative, Bacillus. Homology and phylogenetic tree analysis of 16S rRNA showed that S1 isolate has 91% of sequence similarity with that of Enterobacter cloacae. 91% sequence homology shown in this study proved that the S1 isolate is probably either a new species or another genus of Enterobacteriaceae.

Conclusion: Current study suggests that cellulose hydrolytic bacteria isolated from rumen fluid of Aceh cattle on Bushnell Haas medium-carboxymethylcellulose agar, and some potent cellulose degrading bacteria have been identified. Keywords: Aceh cattle, cellulose, cellulolytic bacteria, Enterobacteriaceae, rumen.

Keywords: Aceh cattle, cellulose, cellulolytic bacteria, Enterobacteriaceae, rumen.

How to cite this article: Sari WN, Safika, Darmawi, Fahrimal Y (2017) Isolation and identification of a cellulolytic Enterobacter from rumen of Aceh cattle, Veterinary World, 10(12): 1515-1520.

Received: 25-08-2017 Accepted: 24-11-2017 Published online: 26-12-2017

Corresponding author: Safika E-mail: fikakhan@yahoo.com

DOI: 10.14202/vetworld.2017.1515-1520

Copyright: Sari, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.