Open Access
Research (Published online: 26-12-2017)
16. Isolation and identification of a cellulolytic Enterobacter from rumen of Aceh cattle
Wenny Novita Sari, Safika, Darmawi and Yudha Fahrimal
Veterinary World, 10(12): 1515-1520

Wenny Novita Sari: Postgraduate student of Mathematics and Applied Sciences, Syiah Kuala University, Darussalam, Banda Aceh 23111, Indonesia.
Safika: Department of Veterinary Infectious Diseases and Veterinary Public Health, Faculty of Veterinary Medicine, Bogor Agricultural University, Jalan Agatis IPB, Darmaga, Bogor, Indonesia.
Darmawi: Microbiology Laboratory, Faculty of Veterinary Medicine, Syiah Kuala University, Darussalam, Banda Aceh 23111, Indonesia.
Yudha Fahrimal: Parasitology Laboratory, Faculty of Veterinary, Syiah Kuala University, Darussalam, Banda Aceh 23111, Indonesia.

doi: 10.14202/vetworld.2017.1515-1520

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Article history: Received: 25-08-2017, Accepted: 24-11-2017, Published online: 26-12-2017

Corresponding author: Safika


Citation: Sari WN, Safika, Darmawi, Fahrimal Y (2017) Isolation and identification of a cellulolytic Enterobacter from rumen of Aceh cattle, Veterinary World, 10(12): 1515-1520.

Aim: The aim of this study was to isolate and identify a cellulolytic bacterium from the rumen fluid of Aceh's cattle. Biodegradation by cellulolytic rumen bacteria can be used as a source of cellulolytic bacteria that act to degrade feed fibrous material so as to improve the quality of nutrients and digestibility of feed ingredients at a cheaper price than the use of commercial cellulase enzymes.

Materials and Methods: Samples were collected from rumen fluid of Aceh's cattle in Abattoirs (RPH) of Banda Aceh city, Indonesia, isolation, and screening of cellulolytic bacteria were done in Microbiology Laboratory, Faculty of Veterinary Medicine, Syiah Kuala University, Banda Aceh, Indonesia.

Results: The S1 isolates showed ±2.5 cm of clear zone diameter. Microscopically, this strain was found to be a Gram-negative, Bacillus. Homology and phylogenetic tree analysis of 16S rRNA showed that S1 isolate has 91% of sequence similarity with that of Enterobacter cloacae. 91% sequence homology shown in this study proved that the S1 isolate is probably either a new species or another genus of Enterobacteriaceae.

Conclusion: Current study suggests that cellulose hydrolytic bacteria isolated from rumen fluid of Aceh cattle on Bushnell Haas medium-carboxymethylcellulose agar, and some potent cellulose degrading bacteria have been identified.

Keywords: Aceh cattle, cellulose, cellulolytic bacteria, Enterobacteriaceae, rumen.


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