Vet World   Vol.10   December-2017  Article-19

Isolation, culture, characterization, and osteogenic differentiation of canine endometrial mesenchymal stem cell

Background and Aim: In this study, the canine endometrium tissue is characterized for its stem cell properties such as adherence to tissue culture plate (plasticity), short population doubling time, serial clonal passaging, long-term culturing properties, stem cell marker expression, and multilineage differentiation potential.

Materials and Methods: The present work describes a novel isolation protocol for obtaining mesenchymal stem cells from the uterine endometrium and is compared with cells derived from umbilical cord matrix as a positive control. These cells are clonogenic, can undergo several population doublings in vitro, and can be differentiated to the osteocytes in mature mesenchymal tissues when grown in osteogenic differentiation media as detected by Alizarin Red-S staining.

Results: It is reported for the first time that the cells derived from the canine endometrium (e-multipotent stem cells [MSCs]) were able to differentiate into a heterologous cell type: Osteocytes, thus demonstrating the presence of MSCs. Thus, the endometrium may be told as a potential source of MSCs which can be used for various therapeutic purposes.

Conclusion: The endometrium can be used as a potential source of MSCs, which can be used for various therapeutic purposes. Keywords: Alizarin Red-S staining, endometrium, mesenchymal stem cell, multipotent stem cells marker, osteogenic differentiation media.

Keywords: Alizarin Red-S staining, endometrium, mesenchymal stem cell, multipotent stem cells marker, osteogenic differentiation media.

How to cite this article: Sahoo AK, Das JK, Nayak S (2017) Isolation, culture, characterization, and osteogenic differentiation of canine endometrial mesenchymal stem cell, Veterinary World, 10(12): 1533-1541.

Received: 03-08-2017  Accepted: 04-12-2017     Published online: 30-12-2017

Corresponding author: A. K. Sahoo   E-mail: aks76vet@gmail.com

DOI: 10.14202/vetworld.2017.1533-1541

Copyright: Sahoo, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.