Ocular squamous cell carcinoma in Holstein cows from the South of Brazil

Vet World Vol.10 December-2017 Article-2

Research Article

Veterinary World, 10(12): 1413-1420

https://doi.org/10.14202/vetworld.2017.1413-1420

Gabrielle A. Fornazari¹, Juliana Kravetz¹, Matti Kiupel², Dodd Sledge², Ivan Roque De Barros Filho¹, and Fabiano Montiani-Ferreira¹

1. Graduate School Program in Veterinary Sciences (PPGCV-UFPR), Federal University of Parana, Rua dos Funcionarios, 1540, 80035-050, Juveve, Curitiba-PR, Brazil.
2. Veterinary Diagnostic Laboratory, 4125 Beaumont RD BLDG 0215, Room 152A, Lansing, MI 48910, USA.

Background and Aim: The aim of this study was to investigate 10 cases of bovine ocular squamous cell carcinoma (OSCC) diagnosed in Holstein or Holstein-crosses cows.

Materials and Methods: The investigation was performed exclusively in OSCC cases diagnosed in the State of Parana and Santa Catarina. A combination of two previously existing histopathological classifications systems was used. The tissue samples were tested for immunoexpression of p53 and p16 and polymerase chain reaction (PCR) for bovine herpesvirus and papillomavirus.

Results: A positive correlation between number of mitotic figures and tissue invasion was found. Anaplasia parameters did not correlate well with tumor invasion of deeper tissues and mitotic counts. Six of 10 OSCC cases were in animals with heavily pigmented eyes. Immunoexpression of p53 and p16 was observed in 3 cases each. Bovine herpesvirus and papillomavirus were not detected by PCR.

Conclusion: Our results indicate that OSCC occurrence is most likely multifactorial with genetic, phenotypic, and environmental influences contributing to the pathogenesis of the disease. Keywords: bovine, histologic classification, ophthalmology, p16, p53, tumor behavior.

Keywords: bovine, histologic classification, ophthalmology, p16, p53, tumor behavior.

How to cite this article: Fornazari GA, Kravetz J, Kiupel M, Sledge D, Filho IRDB, Montiani-Ferreira F (2017) Ocular squamous cell carcinoma in Holstein cows from the South of Brazil, Veterinary World, 10(12): 1413-1420.

Received: 05-09-2017 Accepted: 08-11-2017 Published online: 01-12-2017

Corresponding author: Fabiano Montiani-Ferreira E-mail: montiani@ufpr.br

DOI: 10.14202/vetworld.2017.1413-1420

Copyright: Fornazari, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.