Open Access
Research (Published online: 12-12-2017)
6. Prophylactic and immune modulatory influences of Nigella sativa Linn. in broilers exposed to biological challenge
Essam S. Soliman, Rania T. Hamad and Amira Ahmed
Veterinary World, 10(12): 1447-1455

Essam S. Soliman: Department of Animal Hygiene, Zoonosis and Animal Behavior, Faculty of Veterinary Medicine, Suez Canal University, Ismailia 41522, Egypt.
Rania T. Hamad: Department of Pathology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia 41522, Egypt.
Amira Ahmed: Department of Poultry and Rabbit Medicine, Faculty of Veterinary Medicine, Suez Canal University, Ismailia 41522, Egypt.

doi: 10.14202/vetworld.2017.1447-1455

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Article history: Received: 01-08-2017, Accepted: 14-11-2017, Published online: 12-12-2017

Corresponding author: Essam S. Soliman


Citation: Soliman ES, Hamad RT, Ahmed A (2017) Prophylactic and immune modulatory influences of Nigella sativa Linn. in broilers exposed to biological challenge, Veterinary World, 10(12): 1447-1455.

Background and Aim: Prophylaxis and disease prevention is an essential strategy among biorisk management in poultry farms that stimulate and maintain the birds' immunity. The aim of this study was to investigate the prophylactic, and immune-stimulant influence of Nigella sativa Linn. in broilers under biological stress.

Materials and Methods: A total of 250 1-day-old (ross) chicks were divided into 5 groups; four of which were supplemented with 1.4%, 2.8%, 4.2%, and 5.6% N. sativa Linn., respectively. The four supplemented groups were challenged with Escherichia coli O157:H7 1.5x108 at a 14th day old. A total of 1050 samples (150 serum, 150 swab, and 750 organ samples) were collected and examined.

Results: A highly significant increase (p<0.01) in 5.6% N. sativa Linn. supplemented group in performance traits (body weight, weight gain, and performance index), biochemical parameters (proteinogram, liver enzymes, and creatinine), immunoglobulins concentration, and immune organs' weight. Meanwile, liver showed improvement of histoarchitecture without fibrosis. Heart showed a mild pericarditis with a mild degree of hydropic degeneration. Bursa, thymus, and spleen showed lymphoid hyperplasia.

Conclusion: A concentration of 5.6% N. sativa Linn. in broiler's feed can improve the immune response and subsequent resistance of broilers against diseases.

Keywords: Broiler, Escherichia coli, histopathology, Nigella sativa Linn., preventive.


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