Open Access
Research (Published online: 14-12-2017)
9. Prevalence of carrier state theileriosis in lactating cows
Niranjana Sahoo, Bikash Kumar Behera, Hemant Kumar Khuntia and Manojita Dash
Veterinary World, 10(12): 1471-1474

Niranjana Sahoo: Department of Epidemiology & Preventive Medicine, College of Veterinary Science & Animal Husbandry, Orissa University of Agriculture and Technology, Bhubaneswar, 751003, Odisha, India.
Bikash Kumar Behera: Centre for Wildlife Health, College of Veterinary Science & Animal Husbandry, Orissa University of Agriculture and Technology, Bhubaneswar, 751003, Odisha, India.
Hemant Kumar Khuntia: ICMR-Regional Medical Research Centre , Department of Health Research, Ministry of Health & Family Welfare, Government of India, Bhubaneswar, 751023, Odisha, India.
Manojita Dash: Centre for Wildlife Health, College of Veterinary Science & Animal Husbandry, Orissa University of Agriculture and Technology, Bhubaneswar, 751003, Odisha, India.

doi: 10.14202/vetworld.2017.1471-1474

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Article history: Received: 20-07-2017, Accepted: 02-11-2017, Published online: 14-12-2017

Corresponding author: Niranjana Sahoo


Citation: Sahoo N, Behera BK, Khuntia HK, Dash M (2017) Prevalence of carrier status theileriosis in lactating cows, Veterinary World, 10(12): 1471-1474.

Aim: The objective of this study was to examine the carrier status of theileriosis among apparently healthy cross-bred jersey cattle population of Odisha using conventional blood smear examination and polymerase chain reaction (PCR).

Materials and Methods: A total of 34 apparently healthy cross-bred Jersey lactating cows were considered in this study. Blood samples were subjected to microscopic examination after staining with Giemsa stain and PCR based molecular diagnosis using two sets of primer, i.e., N516/N517 and TorF1/TorF2 specific for Theileria annulata and Theileria orientalis, respectively.

Results: Examination of blood samples revealed presence of theileria parasites to a magnitude of 20.59% for T. annulata, 8.82% for T. orientalis, and 2.94% for both.

Conclusion: Molecular diagnosis was found to be much more sensitive than conventional method for diagnosis of theileriosis. T. annulata was found to be the predominant species affecting the exotic cattle. T. orientalis was detected in apparently healthy cows.

Keywords: bovine theileriosis, carrier state, polymerase chain reaction, Theileria annulata, Theileria orientalis.


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