Vet World Vol.10 December-2017 Article-9
Research Article
Veterinary World, 10(12): 1471-1474
https://doi.org/10.14202/vetworld.2017.1471-1474
Prevalence of carrier state theileriosis in lactating cows
2. Centre for Wildlife Health, College of Veterinary Science & Animal Husbandry, Orissa University of Agriculture and Technology, Bhubaneswar, 751003, Odisha, India.
3. ICMR-Regional Medical Research Centre , Department of Health Research, Ministry of Health & Family Welfare, Government of India, Bhubaneswar, 751023, Odisha, India.
Background and Aim: The objective of this study was to examine the carrier status of theileriosis among apparently healthy cross-bred jersey cattle population of Odisha using conventional blood smear examination and polymerase chain reaction (PCR).
Materials and Methods: A total of 34 apparently healthy cross-bred Jersey lactating cows were considered in this study. Blood samples were subjected to microscopic examination after staining with Giemsa stain and PCR based molecular diagnosis using two sets of primer, i.e., N516/N517 and TorF1/TorF2 specific for Theileria annulata and Theileria orientalis, respectively.
Results: Examination of blood samples revealed presence of theileria parasites to a magnitude of 20.59% for T. annulata, 8.82% for T. orientalis, and 2.94% for both.
Conclusion: Molecular diagnosis was found to be much more sensitive than conventional method for diagnosis of theileriosis. T. annulata was found to be the predominant species affecting the exotic cattle. T. orientalis was detected in apparently healthy cows. Keywords: bovine theileriosis, carrier state, polymerase chain reaction, Theileria annulata, Theileria orientalis.
Keywords: bovine theileriosis, carrier state, polymerase chain reaction, Theileria annulata, Theileria orientalis.
How to cite this article: Sahoo N, Behera BK, Khuntia HK, Dash M (2017) Prevalence of carrier status theileriosis in lactating cows, Veterinary World, 10(12): 1471-1474.
Received: 20-07-2017 Accepted: 02-11-2017 Published online: 14-12-2017
Corresponding author: Niranjana Sahoo E-mail: niranjanasahoo@hotmail.com
DOI: 10.14202/vetworld.2017.1471-1474
Copyright: Sahoo, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.