Hemogram responses in goats toward challenged with Corynebacterium pseudotuberculosis and its immunogen mycolic acids

Research (Published online: 17-06-2017)

14. Hemogram responses in goats toward challenged with Corynebacterium pseudotuberculosis and its immunogen mycolic acids

Mohammed Naji Odhah, Faez Firdaus Jesse Abdullah, Abd Wahid Haron, Mohd. Azmi Mohd. Lila, Mohd. Zamri-Saad, Zaid Khuder, Idris Umar Hambali, Muhammed Umar and Wessam Monther Saleh

Veterinary World, 10(6): 655-661

Mohammed Naji Odhah: Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia; Department of Veterinary Medicine, Faculty of Agriculture and Veterinary Medicine, Thamar University, Dhamar, Yemen.

Faez Firdaus Jesse Abdullah: Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia; Department of Veterinary Clinical Studies, Research Center for Ruminant Disease, Universiti Putra Malaysia 43400 Serdang Selangor, Malaysia.

Abd Wahid Haron: Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.

Mohd. Azmi Mohd. Lila: Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.

Mohd. Zamri-Saad: Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.

Zaid Khuder: Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.

Idris Umar Hambali: Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia; Department of Veterinary Public Health and Preventive Medicine, University of Maiduguri, Nigeria.

Muhammed Umar: Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia; Department of Animal Reproduction, Faculty of Verterinary and Animal Sciences, University of Agriculture, Water and Marine Sciences, Uthal, Baluchistan, Pakistan.

Wessam Monther Saleh: Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia; Department of Internal and Preventive Medicine, Faculty of Veterinary Medicine, University of Basra, Basra, Iraq.

doi: 10.14202/vetworld.2017.655-661

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Article history: Received: 25-11-2016, Accepted: 21-03-2017, Published online: 17-06-2017

Corresponding author: Faez Firdaus Jesse Abdullah

E-mail: jesse@upm.edu.my

Citation: Odhah MN, Abdullah FFJ, Haron AW, Mohd. Lila MA, Zamri-Saad M, Khuder Z, Hambali IU, Umar M, Saleh WM (2017) Hemogram responses in goats toward challenged with Corynebacterium pseudotuberculosis and its immunogen mycolic acids, Veterinary World, 10(6): 655-661.

Abstract

Aim: This study was conducted to analyze the changes in blood profile of goats inoculated with Corynebacterium pseudotuberculosis and its immunogen mycolic acid (MA) extract.

Materials and Methods: A total of 12 clinically healthy crossbred Boer female goats were divided into three groups; A, B and C (4 goats each per group). Group A was inoculated with 2 ml sterile phosphate buffered saline via intradermal route as the negative control group whilst Group B was inoculated with 2 ml of MA extract (1 g/ml) intradermally and Group C was then inoculated with 2 ml (1x10⁹) colony forming unit of active C. pseudotuberculosis intradermally. Blood sample was collected aseptically from the jugular vein periodically for complete blood count (CBC) analysis throughout the experimental period (3 months).

Results: A significant decrease (p<0.05) was observed in red blood cells, hemoglobin (Hb), packed cell volume, mean corpuscular volume and mean corpuscular Hb concentration in Groups B and C as compared to the control while WBCs, neutrophil, lymphocyte and basophil showed a significant increase (p<0.05) as compared to the control.

Conclusion: The inoculation of C. pseudotuberculosis and MA resulted in a significant change in the CBC, thereby, indicating that MA has a role in caseous lymphadenitis pathogenesis.

Keywords: complete blood count, Corynebacterium pseudotuberculosis, goat, hematology, mycolic acid.

References

1. Abdullah, F.F.J., Latif, N.A.A., Chung, E.L.T., Aimi, S., Sarah, M.Z.S., Haron, A.W., Lila, M.A.M., Zakaria, Z. and Norsidin, M.J. (2015) Changes in the reproductive hormones of non-pregnant does infected intradermally with Corynebacterium pseudotuberculosis in chronic form. Int. J. Livest. Res., 5(7): 33-40. [Crossref]

2. Adza-Rina, N., Zamri-Saad, M., Jesse, F.F.A., Saharee, A.A., Haron., A.W. and Shahirudin, S. (2013) Clinical and pathological changes in goats inoculated Corynebacterium pseudotuberculosis by intradermal, intranasal and oral routes. Online J. Vet. Res., 17(2): 73-83.

3. Othman, A.M, Abdullah, F.F.J., Nordi, M., Rina, N.A., Ilyasu, Y., Zamri-Saad, M.,Wahid, A.H., Saharee, A.A. and Mohd-Azmi, M.L. (2014) Haemotological, biochemical and serum electrolyte changes in non-pregnant boer does inoculated with Corynebacterium pseudotuberculosis via various routes. IOSR J. Agric. Vet. Sci., 7(10): 5-8. [Crossref]

4. Zeru, F. and Kahsay, A.G. (2014) Caseous lymphadenitis in goats from Borena range Land South Ethiopia slaughtered at luna export abattoir. J. Vet. Med. Anim. Health, 6(6): 168-173. [Crossref]

5. Abdullah, F.F.J., Osman, A.Y., Adamu, L., Zakaria, Z., Abdullah, R., Saad, M.Z. and Saharee, A.A. (2013) Acute phase protein profiles in calves following infection with whole cell, lipopolysaccharides, and outer membrane protein extracted from Pasteurella multocida Type B: 2. Asian J. Anim. Vet. Adv., 8(4): 655-662. [Crossref]

6. Ashfaq, M. and Campbell, S.A. (1979) Survey of caseous lymphadenitis and its etiology in goats in the United States. Vet. Med. Small Anim. Clin., 74(8): 1161. [PubMed]

7. Junior, J.P., Oliveira, A., Alves, F., Silva, L., Rabelo, S. and Mota, R. (2006) Corynebacterium pseudotuberculosis experimental infection of goats mamary gland. Arq. Inst. Biol. Sao Paulo, 73(4): 395-400.

8. Asano, S. (2012) Granulomatous lymphadenitis. J. Clin. Exp. Hematopathol., 52(1): 1-16. [Crossref]

9. Latif, N.A.A., Abdullah, F.F.J., Othman, A.M., Rina, A., Chung, E.L.T., Zamri-Saad, M., Saharee, A.A., Haron, A.W. and Lila, M.A.M. (2015) Isolation and detection of Corynebacterium pseudotuberculosis in the reproductive organs and associated lymph nodes of non-pregnant does experimentally inoculated through intradermal route in chronic form. Vet. World, 8(7): 924. [Crossref]

10. Jeber, Z., Mohdjin, Z., Jesse, F., Saharee, A., Sabri, J., Yusoff, R. and Wahid, H. (2016) Influence of Corynebacterium pseudotuberculosis infection on level of acute phase proteins in goats. BMC Vet. Res., 12(1): 1. [Crossref] [PubMed] [PMC]

11. Mahmood, Z., Jesse, F., Saharee, A., Jasni, S., Yusoff, R. and Wahid, H. (2015) Assessment of blood changes post-challenge with Corynebacterium pseudotuberculosis and its exotoxin (phospholipase D): A comprehensive study in goat. Vet. World, 8(9): 1105. [Crossref]

12. Mahmood, Z.K., Jin, Z.A.M., Jesse, F.F., Saharee, A.A., Sabri, J., Yusoff, R. and Haron, A.W. (2016) Relationship between the Corynebacterium pseudotuberculosis, phospholipase D inoculation and the fertility characteristics of crossbred Boer bucks. Livest. Sci., 191: 12-21. [Crossref]

13. Butler, W., Ahearn, D. and Kilburn, J. (1986) High-performance liquid chromatography of mycolic acids as a tool in the identification of Corynebacterium, Nocardia, Rhodococcus, and Mycobacterium species. J. Clin. Microbiol., 23(1): 182-185. [PubMed] [PMC]

14. Collins, M.D., Burton, R.A. and Jones, D. (1988) Corynebacterium amycolatum sp. Nov. A new mycolic acid-less Corynebacterium species from human skin. FEMS Microbiol. Lett., 49(3): 349-352. [Crossref]

15. Marrakchi, H., Laneelle, M.A. and Daffe, M. (2014) Mycolic acids: Structures, biosynthesis, and beyond. Chem. Biol., 21(1): 67-85. [Crossref] [PubMed]

16. Vasco, A. (2012) Corynebacterium pseudotuberculosis: Immunological responses in animal models and zoonotic potential. J. Clin. Cell. Immunol., 1(S4): 5.

17. Hard, G.C. (1975) Comparative toxic effect of the surface lipid of Corynebacterium ovis on peritoneal macrophages. Infect. Immun., 12(6): 1439-1449. [PubMed] [PMC]

18. Gotoh, K., Mitsuyama, M., Imaizumi, S., Kawamura, I. and Yano, I. (1991) Mycolic acid-containing glycolipid as a possible virulence factor of rhodococcus equi for mice. Microbiol. Immunol., 35(3): 175-185. [Crossref]

19. Ozkanlar, Y., Aktas, M., Kaynar, O., Ozkanlar, S. and Kireccl, E. (2012) Bovine respiratory disease in naturally infected calves: Clinical signs, blood gases and cytokine response. Rev. Med. Vet., 163: 123-130.

20. Pazatsen, R., Shun, S., Abdullah, F.F.J., Saad, M.Z. and Haron, A. (2012) Clinical Response and Pathological Changes Associated with Corynebacterium pseudotuberculosis Infection in Mice. UPM Serdang, Malaysia.

21. Muckle, C.A. and Gyles, C. (1983) Relation of lipid content and exotoxin production to virulence of Corynebacterium pseudotuberculosis in mice. Am. J. Vet. Res., 44(6): 1149-1153. [PubMed]

22. Jesse, F., Azlan, C., Saharee, A., Murugaiyah, M., Noordin, M., Jasni, S., Ragavan, K., Hassan, M.D. and Haron, A.W. (2008) Control of caseous lymphadenitis (CLA) in goat at UPM farm. In: Proceedings of the 20th Veterinary Association Malaysia (VAM'08).

23. Daffe, M. and Etienne G. (1999) The capsule of Mycobacterium tuberculosis and its implications for pathogenicity. Tuber. Lung Dis., 79(3): 153-169. [Crossref] [PubMed]

24. Kaplanski, G., Marin, V., Montero-Julian, F., Mantovani, A. and Farnarier, C. (2003) IL-6: A regulator of the transition from neutrophil to monocyte recruitment during inflammation. Trends Immunol., 24(1): 25-29. [Crossref]

25. Osman, A.Y., Abdullah, F.F.J., Saharee, A.A., Haron, A.W., Sabri, I. and Abdullah, R. (2012) Haematological and biochemical alterations in mice following experimental infection with whole cell and exotoxin (PLD) extracted from C. pseudotuberculosis. J. Anim. Vet. Adv., 11(24): 4660-4667.

26. Ibtisam, M.A. (2008) Some clinicopathological and pathological studies of C. ovis infection in sheep. Egypt. J. Comp. Pathol. Clin. Pathol., 21(1): 327-343.

27. Russell, D.G., VanderVen, B.C., Lee, W., Abramovitch, R.B., Kim, M.J., Homolka, S., Niemann, S. and Rohde, K.H. (2010) Mycobacterium tuberculosis wears what it eats. Cell Host Microbe, 8(1): 68-76. [Crossref] [PubMed] [PMC]

28. Liu, J., Barry, C.E., Besra, G.S. and Nikaido, H. (1996) Mycolic acid structure determines the fluidity of the mycobacterial cell wall. J. Biol. Chem., 271(47): 29545-29551. [Crossref]

29. Harvey, J. (2010) Erythrocyte biochemistry. Schalm's Veterinary Hematology. 6th ed. Blackwell Publishing Ltd., Ames, USA. p131-135. [PMC]

30. Soucek, A. and Souckova, A. (1973) Toxicity of bacterial sphingomyelinases D. J. Hyg. Epidemiol. Microbiol. Immunol., 18(3): 327-335.

31. Kemp, T.J., Ludwig, A.T., Earel, J.K., Moore, J.M., Vanoosten, R.L., Moses, B., Leidal, K., Nauseef, W.M. and Griffith, T.S. (2005) Neutrophil stimulation with Mycobacterium bovis bacillus Calmette-Guerin (BCG) results in the release of functional soluble TRAIL/Apo-2L. Blood, 106(10): 3474-3482. [Crossref] [PubMed] [PMC]

32. Valli, V. and Parry, B. (1993) Caseous lymphadenitis. Pathol. Domest. Anim., 3: 238-240.

33. Wernery, U. (2012) Caseous lymphadenitis (Pseudotuberculosis) in camelids. J. Camel Pract. Res., 19(1): 21-27.

34. Desvignes, L., Weidinger, C., Shaw, P., Vaeth, M., Ribierre, T., Liu, M., Fergus, T., Kozhaya, L., McVoy, L., Unutmaz, D., Ernst, J.D. and Feske, S. (2015) STIM1 controls T cell-mediated immune regulation and inflammation in chronic infection. J. Clin. Invest., 125(6): 2347. [Crossref]

35. Kim, Y.J., Kim, H.J., Jeong, S.K., Lee, S.H., Kang, M.J., Yu, H.S., Jung, Y.H., Seo, J.H., Kim, B.J., Yu, J., Park, S.J., Lee, Y.C. and Hong, S.J. (2014) A novel synthetic mycolic acid inhibits bronchial hyperresponsiveness and allergic inflammation in a mouse model of asthma. Allergy Asthma Immunol. Res., 6(1): 83-88. [Crossref] [PubMed] [PMC]

36. Dupont, C., Viljoen, A., Dubar, F., Blaise, M., Bernut, A., Pawlik, A., Bouchier, C., Brosch, R., Guerardel, Y., Lelievre, J., Ballell, L., Herrmann, J.L., Biot, C. and Kremer, L. (2016) A new piperidinol derivative targeting mycolic acid transport in Mycobacterium abscessus. Mol. Microbiol., 101(3): 515-529. [Crossref] [PubMed]

37. Li, W., Obregon-Henao, A., Wallach, J.B., North, E.J., Lee, R.E., Gonzalez-Juarrero, M., Schnappinger, D. and Jackson, M. (2016) Therapeutic potential of the Mycobacterium tuberculosis mycolic acid transporter, MmpL3. Antimicrob. Agents Chem., 60(9): 5198-5207. [Crossref] [PubMed] [PMC]