Vet World Vol.10 June-2017 Article-22
Research Article
Veterinary World, 10(6): 711-715
https://doi.org/10.14202/vetworld.2017.711-715
Honey can repairing damage of liver tissue due to protein energy malnutrition through induction of endogenous stem cells
2. Department of Veterinary Anatomy, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, East Java, Indonesia.
Background and Aim: This study was to evaluate effect of honey in repairing damage of liver tissue due to energy protein malnutrition and in mobilization of endogenous stem cells.
Materials and Methods: Male mice model of degenerative liver was obtained through food fasting but still have drinking water for 5 days. It caused energy protein malnutrition and damage of liver tissue. The administration of 50% (v/v) honey was performed for 10 consecutive days, while the positive control group was fasted and not given honey and the negative control not fasted and without honey. Observations of regeneration the liver tissue based on histologically examination, observation of Hsp70 expression, and homing signal based on vascular endothelial growth factor-1 (VEGF-1) expression using immunohistochemistry technique. Observation on expression of CD34 and CD45 as the marker of auto mobilization of hematopoietic stem cells using flow cytometry technique.
Results: There is regeneration of the liver tissue due to protein energy malnutrition, decrease of Hsp70 expression, increase of VEGF-1 expression, and high expression of CD34 and CD45.
Conclusion: Honey can improve the liver tissue based on: (1) Mobilization of endogenous stem cells (CD34 and CD45); (2) Hsp70 and VEGF-1 expressions as regeneration marker of improvement, and (3) regeneration histologically of liver tissue. Keywords: endogenous stem cells, honey, liver tissue, protein energy malnutrition, regeneration.
Keywords: endogenous stem cells, honey, liver tissue, protein energy malnutrition, regeneration.
How to cite this article: Prasetyo RH, Hestianah EP (2017) Honey can repairing damage of liver tissue due to protein energy malnutrition through induction of endogenous stem cells, Veterinary World, 10(6): 711-715.
Received: 26-10-2016 Accepted: 08-05-2017 Published online: 29-06-2017
Corresponding author: R. Heru Prasetyo E-mail: rheru_prasetyo@yahoo.co.id
DOI: 10.14202/vetworld.2017.711-715
Copyright: Prasetyo and Hestianah, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.