Open Access
Research (Published online: 29-06-2017)
22. Honey can repairing damage of liver tissue due to protein energy malnutrition through induction of endogenous stem cells
R. Heru Prasetyo and Eka Pramyrtha Hestianah
Veterinary World, 10(6): 711-715

R. Heru Prasetyo: Department of Parasitology, Faculty of Medicine, Universitas Airlangga, Surabaya, East Java, Indonesia.
Eka Pramyrtha Hestianah: Department of Veterinary Anatomy, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, East Java, Indonesia.

doi: 10.14202/vetworld.2017.711-715

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Article history: Received: 26-10-2016, Accepted: 08-05-2017, Published online: 29-06-2017

Corresponding author: R. Heru Prasetyo


Citation: Prasetyo RH, Hestianah EP (2017) Honey can repairing damage of liver tissue due to protein energy malnutrition through induction of endogenous stem cells, Veterinary World, 10(6): 711-715.

Aim: This study was to evaluate effect of honey in repairing damage of liver tissue due to energy protein malnutrition and in mobilization of endogenous stem cells.

Materials and Methods: Male mice model of degenerative liver was obtained through food fasting but still have drinking water for 5 days. It caused energy protein malnutrition and damage of liver tissue. The administration of 50% (v/v) honey was performed for 10 consecutive days, while the positive control group was fasted and not given honey and the negative control not fasted and without honey. Observations of regeneration the liver tissue based on histologically examination, observation of Hsp70 expression, and homing signal based on vascular endothelial growth factor-1 (VEGF-1) expression using immunohistochemistry technique. Observation on expression of CD34 and CD45 as the marker of auto mobilization of hematopoietic stem cells using flow cytometry technique.

Results: There is regeneration of the liver tissue due to protein energy malnutrition, decrease of Hsp70 expression, increase of VEGF-1 expression, and high expression of CD34 and CD45.

Conclusion: Honey can improve the liver tissue based on: (1) Mobilization of endogenous stem cells (CD34 and CD45); (2) Hsp70 and VEGF-1 expressions as regeneration marker of improvement, and (3) regeneration histologically of liver tissue.

Keywords: endogenous stem cells, honey, liver tissue, protein energy malnutrition, regeneration.


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