Vet World Vol.10 June-2017 Article-23
Research Article
Veterinary World, 10(6): 716-720
https://doi.org/10.14202/vetworld.2017.716-720
Virulence gene profiles of Arcobacter species isolated from animals, foods of animal origin, and humans in Andhra Pradesh, India
2. Department of Veterinary Microbiology, NTR College of Veterinary Science, Gannavaram, Andhra Pradesh, India.
3. Department of Veterinary Microbiology, College of Veterinary Science, Tirupati, Andhra Pradesh, India.
Background and Aim: This study aimed to detect putative virulence genes in Arcobacter species of animal and human origin.
Materials and Methods: A total of 41 Arcobacter isolates (16 Arcobacter butzleri, 13 Arcobacter cryaerophilus, and 12 Arcobacter skirrowii) isolated from diverse sources such as fecal swabs of livestock (21), raw foods of animal origin (13), and human stool samples (7) were subjected to a set of six uniplex polymerase chain reaction assays targeting Arcobacter putative virulence genes (ciaB, pldA, tlyA, mviN, cadF, and cj1349).
Results: All the six virulence genes were detected among all the 16 A. butzleri isolates. Among the 13 A. cryaerophilus isolates, cadF, ciaB, cj1349, mviN, pldA, and tlyA genes were detected in 61.5, 84.6, 76.9, 76.9, 61.5, and 61.5% of isolates, respectively. Among the 12 A. skirrowii isolates, cadF, ciaB, cj1349, mviN, pldA, and tlyA genes were detected in 50.0, 91.6, 83.3, 66.6, 50, and 50% of isolates, respectively.
Conclusion: Putative virulence genes were detected in majority of the Arcobacter isolates examined. The results signify the potential of Arcobacter species as an emerging foodborne pathogen. Keywords: Arcobacter, Arcobacter butzleri, Arcobacter cryaerophilus, Arcobacter skirrowii, polymerase chain reaction, virulence genes.
Keywords: Arcobacter, Arcobacter butzleri, Arcobacter cryaerophilus, Arcobacter skirrowii, polymerase chain reaction, virulence genes.
How to cite this article: Sekhar MS, Tumati SR, Chinnam BK, Kothapalli VS, Sharif NM (2017) Virulence gene profiles of Arcobacter species isolated from animals, foods of animal origin, and humans in Andhra Pradesh, India, Veterinary World, 10(6): 716-720.
Received: 09-03-2017 Accepted: 12-05-2017 Published online: 30-06-2017
Corresponding author: M. Soma Sekhar E-mail: somasekharmadupuru@gmail.com
DOI: 10.14202/vetworld.2017.716-720
Copyright: Sekhar, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.