Phenotypic and genotypic characterization of methicillin-resistant Staphylococcus aureus from bovine mastitis

Vet World Vol.10 March-2017 Article-16

Research Article

Veterinary World, 10(3): 363-367

https://doi.org/10.14202/vetworld.2017.363-367

S. Hamid¹, Mohd Altaf Bhat¹, Irfan Ahmad Mir¹, Anil Taku¹, Gulzar Ahmad Badroo¹, Salik Nazki², and Andleeb Malik¹

1. Bacteriology Laboratory, Division of Veterinary Microbiology and Immunology, Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu, Jammu and Kashmir, India.
2. Laboratory of Veterinary Immunology, College of Veterinary Medicine, Chonbuk National University, Iksan, South Korea.

Background and Aim: This study was conducted to determine the occurrence of methicillin-sensitive and Staphylococcus aureus (MSSA) methicillin-resistant S. aureus (MRSA) from bovine mastitis and to characterize them with respect to antibiotic resistance gene mecA.

Materials and Methods: A total of 160 mastitic milk samples were screened for the presence of S. aureus. The presumptive positive isolates were confirmed using nuc and 23S rRNA gene-based polymerase chain reaction. All the confirmed isolates were subjected to in vitro antibiogram using a number of antibiotics. Isolates which showed resistance against methicillin were characterized for the presence of mecA gene.

Results: Out of the total 160 milk samples, 36 (22.5%) samples yielded S. aureus. The in vitro antibiogram revealed that 16.6% S. aureus isolates were resistant to all antibiotics screened for and 5.5% isolates were sensitive to all of them. Furthermore, the study found 94.4%, 83.3%, 77.7%, 66.6%, 50%, and 27.7% of S. aureus isolates resistant to penicillin, ampicillin, amoxicillin-sulbactam, enrofloxacin, ceftriaxone, and methicillin, respectively. Out of the 36 S. aureus isolates, only 6 (16.6%) isolates were confirmed as MRSA while rest were MSSA.

Conclusion: The higher occurrence of S. aureus-mediated mastitis was concluded due to improper hygienic and poor farm management. The multiple drug resistance reveals the indiscriminate use of drugs and presence of methicillin resistance gene determinant is an alarming situation as such infections are difficult to treat. Keywords: bovine, mastitis, methicillin-resistant Staphylococcus aureus, mecA, methicillin-sensitive Staphylococcus aureus, Staphylococcus aureus.

Keywords: bovine, mastitis, methicillin-resistant Staphylococcus aureus, mecA, methicillin-sensitive Staphylococcus aureus, Staphylococcus aureus.

How to cite this article: Hamid S, Bhat MA, Mir IA, Taku A, Badroo GA, Nazki S, Malik A (2017) Phenotypic and genotypic characterization of methicillin-resistant Staphylococcus aureus from bovine mastitis, Veterinary World, 10(3): 363-367.

Received: 01-11-2016 Accepted: 14-02-2017 Published online: 30-03-2017

Corresponding author: Irfan Ahmad Mir E-mail: mirirfan123@gmail.com

DOI: 10.14202/vetworld.2017.363-367

Copyright: Hamid, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.