Open Access
Research
(Published
online: 04-03-2017)
2.
Effect of kisspeptin on in vitro
maturation of sheep oocytes -
Priyanka Byri, Arunakumari Gangineni, K. Ramachandra Reddy
and K. B. P. Raghavender
Veterinary World, 10(3): 276-280
doi:
10.14202/vetworld.2017.276-280
Priyanka Byri :
Department of
Veterinary Gynaecology and Obstetrics, College of Veterinary
Science, P.V. Narasimha Rao, Telangana Veterinary University,
Rajendra Nagar, Telangana, India.
Arunakumari
Gangineni :
Department of
Veterinary Gynaecology and Obstetrics, College of Veterinary
Science, P.V. Narasimha Rao, Telangana Veterinary University,
Korutla, Telangana, India.
K. Ramachandra
Reddy :
Department of
Veterinary Gynaecology and Obstetrics, College of Veterinary
Science, P.V. Narasimha Rao, Telangana Veterinary University,
Korutla, Telangana, India.
K. B. P.
Raghavender :
Department of
Veterinary Surgery and Radiology, College of Veterinary Science,
P.V. Narasimha Rao, Telangana Veterinary University, Rajendra
Nagar, Hyderabad, India.
Received: 09-10-2016, Accepted: 31-01-2017, Published online:
04-03-2017
Corresponding author:
Arunakumari
Gangineni, e-mail: aruna.gangineni@gmail.com
Citation:
Byri P, Gangineni A, Reddy KR, Raghavender KBP (2017) Effect of
kisspeptin on in vitro maturation of sheep oocytes,
Veterinary World, 10(3): 276-280.
Abstract
Aim:
The aim of
this study was to investigate the effect of kisspeptin (KP) on
in vitro maturation (IVM) of sheep oocytes aspirated from the
ovaries collected from slaughterhouse.
Materials and
Methods:
Two different
experiments were conducted to investigate the effect of KP (5, 10
and 15 μg/ml) alone (experiment 1) or in combination with
follicle-stimulating hormone (FSH), luteinizing hormone (LH), and
Estradiol (E2)
(experiment 2) on IVM of sheep oocytes. Tissue culture medium 199
supplemented with Gentamicin was used as control medium. Good
quality oocytes were randomly allocated into different IVM media
and cultured at 38.5°C in 5% CO2
under humidified atmosphere for 24 h. The oocytes
were evaluated for their cumulus cell expansion (CCE) and
extrusion of the 1st
polar body (PB) at the end of maturation.
Results:
The proportion
of oocytes showing CCE and extrusion of PB was highest when the
oocytes were matured in the medium supplemented with 10 μg/ml of
KP. In experiment 2, oocytes were matured in 12 different
maturation media (G1-G12:
G1:
Control, G2:
KP alone, G3:
FSH, G4:
FSH+KP, G5:
LH, G6:
LH+KP, G7:
E2,
G8:
E2+KP,
G9:
FSH+LH+E2,
G10:
FSH+LH+E2+KP,
G11:
FSH+LH+E2+fetal bovine serum (FBS), G12:
FSH+LH+E2+FBS+KP).
The proportion of oocytes showing cumulus expansion and PB
extrusion was highest (98.33±1.05 and 89.17±2.38) when they were
matured in FSH+LH+E2+FBS+KP
(G12)
and was significantly higher than other groups. The proportion of
CCE and extrusion of PB was significantly increased when KP was
supplemented to FSH and E2,
but no effect was observed with LH. The maturation rates were
significantly increased when FSH, LH, and E2
(G9)
containing media were additionally supplemented with KP (G10).
Conclusion:
This
study demonstrated that the addition of KP (10 μg/ml) to the FSH,
LH, and E2
supplemented media would enhance the sheep oocyte
maturation in vitro.
Keywords:
kisspeptin, in vitro maturation, sheep oocytes.
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