Relative gene expression of fatty acid synthesis genes at 60 days postpartum in bovine mammary epithelial cells of Surti and Jafarabadi buffaloes

Vet World Vol.10 May-2017 Article-1

Research Article

Veterinary World, 10(5): 467-476

https://doi.org/10.14202/vetworld.2017.467-476

Mamta Janmeda¹, Vishnu Kharadi², Gaurav Pandya¹, Balkrishna Brahmkshtri¹, Umed Ramani³, and Kuldeep Tyagi²

1. Department of Animal Genetics and Breeding, College of Veterinary Sciences, Navsari Agricultural University, Navsari, Gujarat, India.
2. Livestock Research Station, Navsari Agricultural University, Navsari, Gujarat, India.
3. Department of Animal Biotechnology, College of Veterinary Sciences, Navsari Agricultural University, Navsari, Gujarat, India.

Background and Aim: Aim of the study was to study the relative gene expression of genes associated with fatty acid synthesis at 60 days postpartum (pp) in bovine mammary epithelial cells (MECs) of Surti and Jafarabadi buffaloes.

Materials and Methods: A total of 10 healthy Surti and Jafarabadi buffaloes of each breed were selected at random from Livestock Research Station, Navsari and Cattle Breeding Farm, Junagadh, Gujarat, respectively, for this study. Milk sample was collected from each selected buffalo at day 60 pp from these two breeds to study relative gene expression of major milk fat genes using non-invasive approach of obtaining primary bovine MECs (pBMEC) from milk samples.

Results: In this study overall, the relative expression of the six major milk lipogenic genes butyrophilin subfamily 1 member A1 (BTN1A1), stearoyl-CoA desaturase (SCD), lipoprotein lipase (LPL), glycerol-3-phosphate acyltransferase mitochondrial (GPAM), acetyl-coenzyme A carboxylase alpha (ACACA), and lipin (LPIN) did not show changes in expression patterns at 60th day of lactation in both Surti and Jafarabadi buffaloes.

Conclusion: The pBMEC can be successfully recovered from 1500 ml of milk of Surti and Jafarabadi buffaloes using antibody-mediated magnetic bead separation and can be further used for recovering RNA for down step quantification of major milk lipogenic gene expression. The relative expression of the six major milk lipogenic genes BTN1A1, SCD, LPL, GPAM, ACACA, and LPIN did not show changes in expression patterns in both Surti and Jafarabadi buffaloes, suggesting expression levels of lipogenic genes are maintained almost uniform till peak lactation without any significant difference. Keywords: buffalo, gene expression, Jafarabadi, milk, Surti.

Keywords: buffalo, gene expression, Jafarabadi, milk, Surti.

How to cite this article: Janmeda M, Kharadi V, Pandya G, Brahmkshtri B, Ramani U, Tyagi K (2017) Relative gene expression of fatty acid synthesis genes at 60 days postpartum in bovine mammary epithelial cells of Surti and Jafarabadi buffaloes, Veterinary World, 10(5): 467-476.

Received: 01-10-2016 Accepted: 22-03-2017 Published online: 02-05-2017

Corresponding author: Mamta Janmeda E-mail: mamtajanmeda@nau.in

DOI: 10.14202/vetworld.2017.467-476

Copyright: Janmeda, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.