Standardization of dot-enzyme-linked immmunosorbent assay for the diagnosis of bovine visceral schistosomiasis

Vet World Vol.10 May-2017 Article-12

Research Article

Veterinary World, 10(5): 536-541

https://doi.org/10.14202/vetworld.2017.536-541

Kommu Sudhakar¹, G. S. Sreenivasa Murthy¹, and Gaddam Rajeshwari²

1. Department of Veterinary Parasitology, College of Veterinary Science, P V Narsimha Rao Telangana Veterinary University, Rajendranagar, Hyderabad, Telangana, India.
2. Department of Veterinary Parasitology, Teaching Veterinary Clinical Complex, College of Veterinary Science, P V Narsimha Rao Telangana Veterinary University, Rajendranagar, Hyderabad, Telangana, India.

Background and Aim: Bovine visceral schistosomiasis has been reported as an important disease entity as it affects animal health, productivity, causes economic losses due to liver condemnation, and produces a high morbidity. This study was conducted to standardize an easy, reliable dot-enzyme-linked immmunosorbent assay (ELISA) for the diagnosis of visceral schistosomiasis caused by Schistosoma spindale and to know the prevalence rate in and around Hyderabad.

Materials and Methods: A dot-ELISA was standardized in the laboratory using whole worm antigen (WWA) and excretory-secretory antigen (ESA) of S. spindale. The standardized test was used for the diagnosis of bovine visceral schistosomiasis at field level. The sensitivity and specificity of the test was compared with counter current immunoelectrophoresis. In total, 288 sera (125 cattle and 163 buffalo) were screened by dot-ELISA.

Results: The dot-ELISA detected 32.63% of infection (94/288) using WWA and 40.62% of infection (117/288) using ESA. In cattle, the prevalence rate was 32.80% (41/125) using WWA and 40.80% (51/125) of infection. Similarly, in buffaloes, the prevalence rate was 32.51% (53/163) using WWA and 40.49% (66/163) of infection using ESA. The overall sensitivity of dot-ELISA was 76.74% and 80.48% with WWA and ESA, respectively, and specificity was 73.3% and 78.57% in WWA and ESA, respectively.

Conclusion: As ante-mortem diagnosis of visceral schistosomiasis is difficult in subclinical conditions, dot-ELISA can be used as a reliable immunodiagnostic test for diagnosis at field level. Keywords: dot-enzyme-linked immmunosorbent assay, excretory-secretory antigen, Schistosoma spindale, whole worm antigen.

Keywords: dot-enzyme-linked immmunosorbent assay, excretory-secretory antigen, Schistosoma spindale, whole worm antigen.

How to cite this article: Sudhakar K, Murthy GSS, Rajeshwari G (2017) Standardization of dot-enzyme-linked immmunosorbent assay for the diagnosis of bovine visceral schistosomiasis, Veterinary World, 10(5): 536-541.

Received: 29-11-2016 Accepted: 30-04-2017 Published online: 21-05-2017

Corresponding author: Kommu Sudhakar E-mail: vety0756@gmail.com

DOI: 10.14202/vetworld.2017.536-541

Copyright: Sudhakar, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.