Open Access
Research (Published online: 21-05-2017)
12. Standardization of dot-enzyme-linked immmunosorbent assay for the diagnosis of bovine visceral schistosomiasis
Kommu Sudhakar, G. S. Sreenivasa Murthy and Gaddam Rajeshwari
Veterinary World, 10(5): 536-541

Kommu Sudhakar: Department of Veterinary Parasitology, College of Veterinary Science, P V Narsimha Rao Telangana Veterinary University, Rajendranagar, Hyderabad, Telangana, India.
G. S. Sreenivasa Murthy: Department of Veterinary Parasitology, College of Veterinary Science, P V Narsimha Rao Telangana Veterinary University, Rajendranagar, Hyderabad, Telangana, India.
Gaddam Rajeshwari: Department of Veterinary Parasitology, Teaching Veterinary Clinical Complex, College of Veterinary Science, P V Narsimha Rao Telangana Veterinary University, Rajendranagar, Hyderabad, Telangana, India.

doi: 10.14202/vetworld.2017.536-541

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Article history: Received: 29-11-2016, Accepted: 30-04-2017, Published online: 21-05-2017

Corresponding author: Kommu Sudhakar

E-mail: vety0756@gmail.com

Citation: Sudhakar K, Murthy GSS, Rajeshwari G (2017) Standardization of dot-enzyme-linked immmunosorbent assay for the diagnosis of bovine visceral schistosomiasis, Veterinary World, 10(5): 536-541.
Abstract

Aim: Bovine visceral schistosomiasis has been reported as an important disease entity as it affects animal health, productivity, causes economic losses due to liver condemnation, and produces a high morbidity. This study was conducted to standardize an easy, reliable dot-enzyme-linked immmunosorbent assay (ELISA) for the diagnosis of visceral schistosomiasis caused by Schistosoma spindale and to know the prevalence rate in and around Hyderabad.

Materials and Methods: A dot-ELISA was standardized in the laboratory using whole worm antigen (WWA) and excretory-secretory antigen (ESA) of S. spindale. The standardized test was used for the diagnosis of bovine visceral schistosomiasis at field level. The sensitivity and specificity of the test was compared with counter current immunoelectrophoresis. In total, 288 sera (125 cattle and 163 buffalo) were screened by dot-ELISA.

Results: The dot-ELISA detected 32.63% of infection (94/288) using WWA and 40.62% of infection (117/288) using ESA. In cattle, the prevalence rate was 32.80% (41/125) using WWA and 40.80% (51/125) of infection. Similarly, in buffaloes, the prevalence rate was 32.51% (53/163) using WWA and 40.49% (66/163) of infection using ESA. The overall sensitivity of dot-ELISA was 76.74% and 80.48% with WWA and ESA, respectively, and specificity was 73.3% and 78.57% in WWA and ESA, respectively.

Conclusion: As ante-mortem diagnosis of visceral schistosomiasis is difficult in subclinical conditions, dot-ELISA can be used as a reliable immunodiagnostic test for diagnosis at field level.

Keywords: dot-enzyme-linked immmunosorbent assay, excretory-secretory antigen, Schistosoma spindale, whole worm antigen.

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