Open Access
Review (Published online: 09-05-2017)
5. An immunological approach of sperm sexing and different methods for identification of X- and Y-chromosome bearing sperm
Shiv Kumar Yadav, Dharmendra Kumar Gangwar, Jarnail Singh, Chiranjeev Kumar Tikadar, V. Vinoth Khanna, Sudha Saini, Sunny Dholpuria, Prabhat Palta, Radhey Shyam Manik, Manoj Kumar Singh and Suresh Kumar Singla
Veterinary World, 10(5): 498-504

Shiv Kumar Yadav: Embryo Biotechnology Laboratory, Animal Biotechnology Centre, ICAR-National Dairy Research Institute, Karnal - 132 001, Haryana, India.
Dharmendra Kumar Gangwar: Embryo Biotechnology Laboratory, Animal Biotechnology Centre, ICAR-National Dairy Research Institute, Karnal - 132 001, Haryana, India.
Jarnail Singh: Embryo Biotechnology Laboratory, Animal Biotechnology Centre, ICAR-National Dairy Research Institute, Karnal - 132 001, Haryana, India.
Chiranjeev Kumar Tikadar: Embryo Biotechnology Laboratory, Animal Biotechnology Centre, ICAR-National Dairy Research Institute, Karnal - 132 001, Haryana, India.
V. Vinoth Khanna: Embryo Biotechnology Laboratory, Animal Biotechnology Centre, ICAR-National Dairy Research Institute, Karnal - 132 001, Haryana, India.
Sudha Saini: Embryo Biotechnology Laboratory, Animal Biotechnology Centre, ICAR-National Dairy Research Institute, Karnal - 132 001, Haryana, India.
Sunny Dholpuria: Embryo Biotechnology Laboratory, Animal Biotechnology Centre, ICAR-National Dairy Research Institute, Karnal - 132 001, Haryana, India.
Prabhat Palta: Embryo Biotechnology Laboratory, Animal Biotechnology Centre, ICAR-National Dairy Research Institute, Karnal - 132 001, Haryana, India.
Radhey Shyam Manik: Embryo Biotechnology Laboratory, Animal Biotechnology Centre, ICAR-National Dairy Research Institute, Karnal - 132 001, Haryana, India.
Manoj Kumar Singh: Embryo Biotechnology Laboratory, Animal Biotechnology Centre, ICAR-National Dairy Research Institute, Karnal - 132 001, Haryana, India.
Suresh Kumar Singla: Embryo Biotechnology Laboratory, Animal Biotechnology Centre, ICAR-National Dairy Research Institute, Karnal - 132 001, Haryana, India.

doi: 10.14202/vetworld.2017.498-504

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Article history: Received: 03-01-2017, Accepted: 15-03-2017, Published online: 09-05-2017

Corresponding author: Suresh Kumar Singla

E-mail: singlasuresh@yahoo.com

Citation: Yadav SK, Gangwar DK, Singh J, Tikadar CK, Khanna VV, Saini S, Dholpuria S, Palta P, Manik RS, Singh MK, Singla SK (2017) An immunological approach of sperm sexing and different methods for identification of X- and Y-chromosome bearing sperm, Veterinary World, 10(5): 498-504.
Abstract
Separation of X- and Y-chromosome bearing sperm has been practiced for selection of desired sex of offspring to increase the profit in livestock industries. At present, fluorescence-activated cell sorter is the only successful method for separation of X- and Y-chromosome bearing sperm. This technology is based on the differences in DNA content between these two types of sperm and has been commercialized for bovine sperm. However, this technology still has problems in terms of high economic cost, sperm damage, and lower pregnancy rates compared to unsorted semen. Therefore, an inexpensive, convenient, and non-invasive approach for sperm sexing would be of benefit to agricultural sector. Within this perspective, immunological sperm sexing method is one of the attractive choices to separate X- and Y-chromosome bearing sperm. This article reviews the current knowledge about immunological approaches, viz., H-Y antigen, sex-specific antigens, and differentially expressed proteins for sperm sexing. Moreover, this review also highlighted the different methods for identification of X- and Y-sperm.

Keywords: differentially expressed proteins, H-Y antigen, sex specific proteins, sperm identification, sperm sexing.

References

1. De Canio, M., Soggiu, A., Piras, C., Bonizzi, L., Galli, A., Urbani, A. and Roncada, P. (2014) Differential protein profile in sexed bovine semen: Shotgun proteomics investigation. Mol. Biosyst., 10: 1264-1271. [Crossref]

2. Yang, W.C., Sang, L., Xiao, Y., Zhang, H.L., Tang, K.Q. and Yang, L.G. (2014) Tentative identification of sex-specific antibodies and their application for screening bovine sperm proteins for sex-specificity. Mol. Biol. Rep., 41: 217-223. [Crossref] [PubMed]

3. Prakash, M.A., Kumaresan, A., Manimaran, A., Joshi, R.K., Layek, S.S., Mohanty, T.K. and Divisha, R.R. (2014) Sexing of spermatozoa in farm animals: A mini review. Adv. Anim. Vet. Sci., 2: 226-232. [Crossref]

4. Sumner, A.T. and Robinson, J.A. (1976) A difference in dry mass between the heads of X-and Y-bearing human spermatozoa. J. Reprod. Fertil., 48: 9-15. [Crossref] [PubMed]

5. Johnson, L.A., Welch, G.R. and Keyvanfar, K. (1993) Gender preselection in humans? How cytometric separation of X and Y spermatozoa for the prevention of X-linked diseases. Hum. Reprod., 8: 1733-1739. [Crossref] [PubMed]

6. Garner, D.L., Gledhill, B.L., Pinkel, D., Lake, S., Stephenson, D., Van Dilla, M.A. and Johnson, L.A. (1983) Quantification of the X-and Y-chromosome-bearing spermatozoa of domestic animals by flow cytometry. Biol. Reprod., 28: 312-321. [Crossref] [PubMed]

7. Johnson, L.A. and Welch, G.R. (1999) Sex preselection: High-speed flow cytometric sorting of X and Y sperm for maximum efficiency. Theriogenology, 52: 1323-1341. [Crossref]

8. Johnson, L.A. (1992) Gender preselection in domestic animals using flow cytometrically sorted sperm. J. Anim. Sci., 70: 8-18. [Crossref]

9. Cui, K.H. and Matthews, C.D. (1993) X larger than Y. Nature, 366: 117-118. [Crossref] [PubMed]

10. Cui, K.H. (1997) Size differences between human X and Y spermatozoa and prefertilization diagnosis. Mol. Hum. Reprod., 3: 61-67. [Crossref] [PubMed]

11. Kiddy, C.A. and Hafs, H.D. (1971) Sex ratio at birth-prospects for control. Am. Soc. Anim. Sci., 4: 104.

12. Hoppe, P.C. and Koo, G.C. (1984) Reacting mouse sperm with monoclonal HY antibodies does not influence sex ratio of eggs fertilized in vitro. J. Reprod. Immunol., 6: 1-9. [Crossref]

13. Penfold, L.M., Holt, C., Holt, W.V., Welch, G.R., Cran, D.G. and Johnson, L.A. (1998) Comparative motility of X and Y chromosome-bearing bovine sperm separated on the basis of DNA content by flow sorting. Mol. Reprod. Dev., 50: 323-327. [Crossref]

14. Hendriksen, P.J.M. (1999) Do X and Y spermatozoa differ in proteins? Theriogenology, 52: 1295-1307. [Crossref]

15. Koundouros, S. and Verma, P. (2012) Significant enrichment of Y-bearing chromosome human spermatozoa using a modified centrifugation technique. Int. J. Androl., 35: 880-886. [Crossref] [PubMed]

16. Han, T.L., Flaherty, S.P., Ford, J.H. and Matthews, C.D. (1993a) Detection of X-and Y-bearing human spermatozoa after motile sperm isolation by swim-up. Fertil. Steril., 60: 1046-1051. [Crossref]

17. Steeno, O., Adimoelja, A. and Steeno, J. (1975) Separation of X-and Y-bearing human spermatozoa with the sephadex gel-filtration method. Andrologia, 7: 95-97. [Crossref] [PubMed]

18. Bennett, D. and Boyse, E.A. (1973) Sex ratio in progeny of mice inseminated with sperm treated with H-Y antiserum. Nature, 246: 308-309. [Crossref] [PubMed]

19. Johnson, L.A., Flook, J.P. and Hawk, H.W. (1989) Sex preselection in rabbits: Live births from X and Y sperm separated by DNA and cell sorting. Biol. Reprod., 41: 199-203. [Crossref]

20. Garner, D.L. and Seidel, G.E. (2008) History of commercializing sexed semen for cattle. Theriogenology, 69: 886-895. [Crossref] [PubMed]

21. Blondin, P., Beaulieu, M., Fournier, V., Morin, N., Crawford, L., Madan, P. and King, W.A. (2009) Analysis of bovine sexed sperm for IVF from sorting to the embryo. Theriogenology, 71: 30-38. [Crossref] [PubMed]

22. Rath, D. and Johnson, L.A. (2008) Application and commercialization of flow cytometrically sex-sorted semen. Reprod. Domest. Anim., 43: 338-346. [Crossref] [PubMed]

23. Rath, D., Barcikowski, S., de Graaf, S., Garrels, W., Grossfeld, R., Klein, S., Knabe, W., Knorr, C., Kues, W., Meyer, H. and Michl, J. (2013) Sex selection of sperm in farm animals: Status report and developmental prospects. Reproduction, 145: R15-R30. [Crossref]

24. Morris, L.H.A. (2005) Challenges facing sex preselection of stallion spermatozoa. Anim. Reprod. Sci., 89: 147-157. [Crossref] [PubMed]

25. O'Brien, J.K. and Robeck, T.R. (2006) Development of sperm sexing and associated assisted reproductive technology for sex preselection of captive bottlenose dolphins (Tursiops truncatus). Reprod. Fertil. Dev., 18: 319-329. [Crossref] [PubMed]

26. Rath, D., Moench-Tegeder, G., Taylor, U. and Johnson, L.A. (2009) Improved quality of sex-sorted sperm: A prerequisite for wider commercial application. Theriogenology, 71: 22-29. [Crossref] [PubMed]

27. Leahy, T., Evans, G., Maxwell, W.M.C. and Marti, J.I. (2010) Seminal plasma proteins do not consistently improve fertility after cervical insemination of ewes with non-sorted or sex-sorted frozen-thawed ram spermatozoa. Reprod. Fertil. Dev., 22: 606-612. [Crossref]

28. Gibb, Z., Morris, L.H.A., Maxwell, W.M.C., Grupen, C.G. (2011) Use of a defined diluents increases the sex-sorting efficiency of stallion sperm. Theriogenology, 75: 610-619. [Crossref] [PubMed]

29. Clulow, J.R., Buss, H., Evans, G., Sieme, H., Rath, D., Morris, L.H.A. and Maxwell, W.M.C. (2012) Effect of staining and freezing media on sortability of stallion spermatozoa and their post-thaw viability after sex-sorting and cryopreservation. Reprod. Domest. Anim., 47: 1-7. [Crossref] [PubMed]

30. Seidel, G.E.Jr. (2003) Sexing mammalian sperm-intertwining of commerce, technology, and biology. Anim. Reprod. Sci., 79: 145-156. [Crossref]

31. Spinaci, M., Ambrogi, M., Volpe, S., Penazzi, P., Galeati, G., and Seren, E. (2005) Assessment of some morphofunctional characteristics of flow-cytometrically sorted and stained boar spermatozoa. Vet. Res. Commun., 22: 207-209. [Crossref] [PubMed]

32. Seidel, G.E. (2014) Update on sexed semen technology in cattle. Animal, 8: 160-164. [Crossref] [PubMed]

33. Boro, P., Naha, B.C., Madkar, A. and Prakash, C. (2016) Sexing of semen in bulls: A mini review. IJAR, 2: 460-462.

34. Chen, X., Yue, Y., He, Y., Zhu, H., Hao, H., Zhao, X., Qin, T. and Wang, D. (2014) Identification and characterization of genes differentially expressed in X and Y sperm using suppression subtractive hybridization and cDNA microarray. Mol. Reprod. Dev., 81: 908-917. [Crossref] [PubMed]

35. Seidel, G.E. and Johnson, L.A. (1999) Sexing mammalian sperm-overview. Theriogenology, 52: 1267-1272. [Crossref]

36. Hendriksen, P.J.M., Welch, G.R., Grootegoed, J.A., van der Lende, T. and Johnson, L.A. (1996) Comparison of detergent-solubilized membrane and soluble proteins from flow cytometrically sorted X-and Y-chromosome bearing porcine spermatozoa by high resolution 2-D electrophoresis. Mol. Reprod. Dev., 45: 342-350. [Crossref]

37. Bradley, M.P. (1989) Immunological sexing of mammalian semen: Current status and future options. J. Dairy Sci., 72: 3372-3380. [Crossref]

38. Prasad, S., Rangasamy, S. and Satheshkumar, S. (2010) Sex preselection in domestic animals-current status and future prospects. Vet. World, 3: 346-348.

39. Zavos, P.M. (1983) Preconception sex determination via intra-vaginal administration of HY antisera in rabbits. Theriogenology, 20: 235-240. [Crossref]

40. Ali, J.I., Eldridge, F.E., Koo, G.C. and Schanbacher, B.D. (1990) Enrichment of bovine X-and Y-chromosome-bearing sperm with monoclonal HY antibody-fluorescence-activated cell sorter. Arch. Androl., 24: 235-245. [Crossref] [PubMed]

41. Ohno, S. and Wachtel, S.S. (1978) On the selective elimination of Y-bearing sperm. Immunogenetics, 7: 13-16. [Crossref] [PubMed]

42. Grant, V.J., Irwin, R.J., Standley, N.T., Shelling, A.N. and Chamley, L.W. (2008) Sex of bovine embryos may be related to mothers' preovulatory follicular testosterone. Biol. Reprod., 78: 812-815. [Crossref] [PubMed]

43. Zhang, L., Du, W., Lin, X., Zhang, A. and Chen, H. (2008) Progesterone and 17β-estradiol, but not follicle stimulating hormone, alter the sex ratio of murine embryos fertilized in vitro. Theriogenology, 69: 961-966. [Crossref] [PubMed]

44. Soares, S.G. and Barbosa, J.E. (2008) Application of recombinant antibody library for screening specific antigens in a bovine sperm cell subpopulation. Livest. Sci., 114: 188-193. [Crossref]

45. Beerli, R.R., Bauer, M., Buser, R.B., Gwerder, M., Muntwiler, S., Maurer, P., Saudan, P. and Bachmann, M.F. (2008) Isolation of human monoclonal antibodies by mammalian cell display. Proc. Natl. Acad. Sci., 105: 14336-14341. [Crossref] [PubMed] [PMC]

46. Blecher, S.R., Howie, R., Li, S., Detmar, J. and Blahut, L.M. (1999) A new approach to immunological sexing of sperm. Theriogenology, 52: 1309-1321. [Crossref]

47. Sang, L., Yang, W.C., Han, L., Liang, A.X., Hua, G.H., Xiong, J.J., Huo, L.J. and Yang, L.G. (2011) An immunological method to screen sex-specific proteins of bovine sperm. J. Dairy Sci., 94: 2060-2070. [Crossref] [PubMed]

48. Li, C., Sun, Y., Yi, K., Li, C., Zhu, X., Chen, L. and Zhou, X. (2011) Detection of the SRY transcript and protein in bovine ejaculated spermatozoa. Asian-Australas. J. Anim. Sci., 24: 1358-1364. [Crossref]

49. Li, C.J., Wang, D. and Zhou, X. (2016) Sperm proteome and reproductive technologies in mammals. Anim. Reprod. Sci., 173: 1-7. [Crossref] [PubMed]

50. Baker, M.A. (2011) The 'omics' revolution and our understanding of sperm cell biology. Asian J. Androl., 13: 6-10. [Crossref] [PubMed] [PMC]

51. Byrne, K., Leahy, T., McCulloch, R., Colgrave, M.L. and Holland, M.K. (2012) Comprehensive mapping of the bull sperm surface proteome. Proteomics, 12: 3559-3579. [Crossref] [PubMed]

52. Chen, X., Zhu, H., Wu, C., Han, W., Hao, H., Zhao, X., Du, W., Qin, T., Liu, Y. and Wang, D. (2012) Identification of differentially expressed proteins between bull X and Y spermatozoa. J. Proteomics, 77: 59-67. [Crossref] [PubMed]

53. Srivastava, N., Jerome, A., Srivastava, S.K., Ghosh, S.K. and Kumar, A. (2013) Bovine seminal PDC-109 protein: An overview of biochemical and functional properties. Anim. Reprod. Sci., 138: 1-13. [Crossref] [PubMed]

54. Hao, Z., Wolkowicz, M.J., Shetty, J., Klotz, K., Bolling, L., Sen, B., Westbrook, V.A., Coonrod, S., Flickinger, C.J. and Herr, J.C. (2002) SAMP32, a testis-specific, isoantigenic sperm acrosomal membrane-associated protein. Biol. Reprod., 66: 735-744. [Crossref] [PubMed]

55. Boyse, E.A. and Old, L.J. (1969) Some aspects of normal and abnormal cell surface genetics. Annu. Rev. Genet., 3: 269-290. [Crossref]

56. Caspersson, T., Farber, S., Foley, G.E., Kudynowski, J., Modest, E.J., Simonsson, E., Wagh, U. and Zech, L. (1968) Chemical differentiation along metaphase chromosomes. Exp. Cell. Res., 49: 219-222. [Crossref]

57. Barlow, P. and Vosa, C.G. (1970) The V-chromosome in human sperm. Nature, 226: 961-962. [Crossref] [PubMed]

58. Thomsen, J.L. and Niebuhr, E. (1986) The frequency of false-positive and false-negative results in the detection of Y-chromosomes in interphase nuclei. Hum. Genet., 73: 27-30. [Crossref] [PubMed]

59. Flaherty, S.P. and Matthews, C.D. (1996) Application of modern molecular techniques to evaluate sperm sex selection methods. Mol. Hum. Reprod., 2: 937-942. [Crossref] [PubMed]

60. Pearson, P.L., Bobrow, M., Vosa, C.G. and Barlow, P.W. (1971) Quinacrine fluorescence in mammalian chromosomes. Nature, 231: 326-329. [Crossref] [PubMed]

61. Cran, D.G. and Johnson, L.A. (1996) The predetermination of embryonic sex using flow cytometrically separated X and Y spermatozoa. Hum. Reprod. Update, 2: 355-363. [Crossref] [PubMed]

62. Welch, D.P., Waldbieser, G.C., Wall, R.J. and Johnson, L.A. (1996) Flow cytometric sperm sorting and PCR to confirm separation of X-and Y-chromosome bearing bovine sperm. Anim. Biotechnol., 6: 131-139. [Crossref]

63. Seidel, G.E.Jr. (2012) Sexing mammalian sperm-where do we go from here? J. Reprod. Dev., 58: 505-509. [Crossref] [PubMed]

64. Maya, O.I., Yamada, K., Hayakawa, H., and Suzuki, H. (2013) Sexing of dog sperm by fluorescence in situ hybridization. J. Reprod. Dev., 59: 92.

65. Colley, A., Buhr, M. and Golovan, S.P. (2008) Single bovine sperm sex typing by amelogenin nested PCR. Theriogenology, 70: 978-983. [Crossref] [PubMed]

66. Wang, D., Zhu, H., Guo, J., Lin, B., Zhang, L., Hao, H., Du, W. and Zhao, X. (2011) A study of a method to assess the purity of sorted bovine semen using rapid single-sperm sexing PCR. J. Anim. Vet. Adv., 10: 750-756. [Crossref]

67. Parati, K., Bongioni, G., Aleandri, R. and Galli, A. (2006) Sex ratio determination in bovine semen: A new approach by quantitative real time PCR. Theriogenology, 66: 2202-2209. [Crossref] [PubMed]

68. Khamlor, T., Pongpiachan, P., Sangsritavong, S. and Chokesajjawatee, N. (2014) Determination of sperm sex ratio in bovine semen using multiplex real-time polymerase chain reaction. Asian Australas. J. Anim. Sci., 27: 1411. [Crossref] [PubMed] [PMC]

69. Tan, Y.J., Mahanem, M.N. and Somarny, W.W.M. (2015) SYBR® green quantitative PCR for sex determination of bovine spermatozoa. J. Trop. Agric. Food Sci., 43: 29-39.

70. Han, T.L., Ford, J.H., Webb, G.C., Flaherty, S.P., Correll, A. and Matthews, C.D. (1993b) Simultaneous detection of X-and Y-bearing human sperm by double fluorescence in situ hybridization. Mol. Reprod. Dev., 34: 308-313. [Crossref] [PubMed]

71. Parrilla, I., Vazquez, J.M., Oliver-Bonet, M., Navarro, J., Yelamos, J., Roca, J. and Martinez, E.A. (2003) Fluorescence in situ hybridization in diluted and flow cytometrically sorted boar spermatozoa using specific DNA direct probes labelled by nick translation. Reproduction, 126: 317-325. [Crossref] [PubMed]

72. Wyrobek, A.J., Robbins, W.A., Tang, C., Kobayashi, A., Baulch, J., Weier, H.U. and Pinkel, D. (1993) Hierarchical organization of human sperm chromatin is a critical factor in the detection of chromosomal aneuploidies by fluorescence in situ hybridization. Am. J. Hum. Genet., 53(3): 130.

73. De Luca, A.C., Manago, S., Ferrara, M.A., Rendina, I., Sirleto, L., Puglisi, R., Balduzzi, D., Galli, A., Ferraro, P. and Coppola, G. (2014) Non-invasive sex assessment in bovine semen by Raman spectroscopy. Laser Phys. Lett., 11: 055604. [Crossref]

74. Manago, S., Coppola, G., Ferrara, M.A., Sirleto, L., Rendina, I., Puglisi, R., Balduzzi, D., Galli, A., Ferraro, P. and De Luca, A.C. (2014) Raman sex sorting of bovine spermatozoa. In: Photonics Technologies, 2014 Fotonica AEIT Italian Conference, IEEE. p1-4. [Crossref]

75. Meister, K., Schmidt, D.A., Brundermann, E. and Havenith, M. (2010) Confocal Raman micro spectroscopy as an analytical tool to assess the mitochondrial status in human spermatozoa. Analyst, 135: 1370-1374. [Crossref] [PubMed]