Open Access
Research (Published online: 30-11-2017)
20. Pathology and polymerase chain reaction detection of ovine progressive pneumonia (maedi) cases in slaughtered sheep in India
Rahul Singh, Pawan Kumar, Rajendra Singh, Kuldeep Dhama, Swati Kumari, Jay Prakash Yadav, Gayatri Kashyap, Karam Pal Singh, Vidya Singh and Monalisa Sahoo
Veterinary World, 10(11): 1401-1406

Rahul Singh: Division of Pathology, ICAR-Indian Veterinary Research Institute, Bareilly, Izatnagar - 243 122, Uttar Pradesh, India.
Pawan Kumar: Division of Pathology, ICAR-Indian Veterinary Research Institute, Bareilly, Izatnagar - 243 122, Uttar Pradesh, India.
Rajendra Singh: Division of Pathology, ICAR-Indian Veterinary Research Institute, Bareilly, Izatnagar - 243 122, Uttar Pradesh, India.
Kuldeep Dhama: Division of Pathology, ICAR-Indian Veterinary Research Institute, Bareilly, Izatnagar - 243 122, Uttar Pradesh, India.
Swati Kumari: Division of Pathology, ICAR-Indian Veterinary Research Institute, Bareilly, Izatnagar - 243 122, Uttar Pradesh, India.
Jay Prakash Yadav: Division of Veterinary Public Health, ICAR-Indian Veterinary Research Institute, Bareilly, Izatnagar - 243 122, Uttar Pradesh, India.
Gayatri Kashyap: Division of Pathology, ICAR-Indian Veterinary Research Institute, Bareilly, Izatnagar - 243 122, Uttar Pradesh, India.
Karam Pal Singh: Division of Pathology, Centre for Animal Disease Research and Diagnosis, ICAR-Indian Veterinary Research Institute, Bareilly, Izatnagar - 243 122, Uttar Pradesh, India.
Vidya Singh: Division of Pathology, ICAR-Indian Veterinary Research Institute, Bareilly, Izatnagar - 243 122, Uttar Pradesh, India.
Monalisa Sahoo: Division of Pathology, ICAR-Indian Veterinary Research Institute, Bareilly, Izatnagar - 243 122, Uttar Pradesh, India.

doi: 10.14202/vetworld.2017.1401-1406

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Article history: Received: 24-07-2017, Accepted: 25-10-2017, Published online: 30-11-2017

Corresponding author: Rajendra Singh

E-mail: rajendra_singh5747@rediffmail.com

Citation: Singh R, Kumar P, Singh R, Dhama K, Kumari S, Yadav JP, Kashyap G, Singh KP, Singh V, Sahoo M (2017) Pathology and polymerase chain reaction detection of ovine progressive pneumonia (maedi) cases in slaughtered sheep in India, Veterinary World, 10(11): 1401-1406.
Abstract

Aim: The small ruminant lentiviruses are known to cause maedi-visna (MV) and caprine arthritis - encephalitis in sheep and goats, typically affecting joints, udder, lungs, and the central nervous system. The diagnosis usually involves serology, clinical signs, immunohistochemistry, and polymerase chain reaction (PCR). In the present study, the histopathologically positive pneumonia cases of MV were confirmed by PCR in lung tissue probably for the first time in India.

Materials and Methods: A total of 888 lungs of adult sheep, aged between 2 and 5 years, were screened during slaughter, of which 121 were found to have pneumonic lesions. The tissues from each pneumonic lung including associated lymph nodes were collected in 10% neutral buffered formalin for histopathology. The frozen tissues of the same were also collected and stored at -20°C for PCR confirmation.

Results: Three of 121 cases of pneumonic lungs of sheep revealed gross and histopathological lesions suggestive of maedi or ovine progressive pneumonia infection. These 3 cases were further confirmed by PCR technique that amplified 291-base pair DNA in the long terminal repeat sequence of MV provirus.

Conclusion: This study suggests the low occurrence of MV virus (MVV) infection in India in naturally affected sheep based on pathomorphological lesions and using the molecular tool of PCR detection of the virus in tissues. Further, a combination of pathomorphology or/and PCR testing might be optimal for detecting the animals infected with MVV.

Keywords: histopathology, maedi-visna, ovine progressive pneumonia, polymerase chain reaction, small ruminant lentiviruses.

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