Open Access
Research (Published online: 24-10-2017)
16. Molecular characterization of Escherichia coli recovered from traditional milk products in Kashan, Iran
Farhad Sharafati Chaleshtori, Navid Mazroii Arani, Esmat Aghadavod, Afshin Naseri and Reza Sharafati Chaleshtori
Veterinary World, 10(10): 1264-1268

Farhad Sharafati Chaleshtori: Department of Microbiology, Medical Plants Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran.
Navid Mazroii Arani: Department of Microbiology Food and Hygiene Control Laboratory, Deputy of Food and Drug, Kashan University of Medical Sciences, Kashan, Iran.
Esmat Aghadavod: Department of Biochemistry, Research Center for Biochemistry and Nutrition in Metabolic Diseases, Kashan University of Medical Sciences, Kashan, Iran.
Afshin Naseri: Department of Nutrition, Research Center for Biochemistry and Nutrition in Metabolic Diseases, Kashan University of Medical Sciences, Kashan, Iran.
Reza Sharafati Chaleshtori: Department of Nutrition, Research Center for Biochemistry and Nutrition in Metabolic Diseases, Kashan University of Medical Sciences, Kashan, Iran.

doi: 10.14202/vetworld.2017.1264-1268

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Article history: Received: 24-06-2017, Accepted: 25-09-2017, Published online: 24-10-2017

Corresponding author: Reza Sharafati Chaleshtori


Citation: Chaleshtori FS, Arani NM, Aghadavod E, Naseri A, Chaleshtori RS (2017) Molecular characterization of Escherichia coli recovered from traditional milk products in Kashan, Iran, Veterinary World, 10(10): 1264-1268.

Aim: Shiga toxigenic Escherichia coli (STEC) strains as emerging groups of foodborne pathogens are responsible for most foodborne illnesses. The aim of this study was to determine the antibiotic resistance pattern in STEC isolated from traditional milk products and their molecular characterization.

Materials and Methods: A total of 116 samples were randomly purchased from local markets in Kashan, Iran, and evaluated for the occurrence of STEC by culturing and molecular methods. The antibiotic resistance of obtained isolates was determined by Kirby Bauer method. Furthermore, isolates were assayed for the presence of Shiga toxins (stx1 and stx2) and intimin gene (eae).

Results: The incidence of E. coli in 60 ice cream, 30 yoghurt, and 26 cheese samples was 8.33%, 10%, and 11.54%, respectively. The findings showed that 11 out of 11 (100%) E. coli had both stx1 and stx2 while eae gene was not found in E. coli isolated of traditional milk products. For E. coli strains carrying stx1 and stx2, highest antibiotic sensitive levels were related to trimethoprim/sulfamethoxazole, norfloxacin, chloramphenicol, and ciprofloxacin, respectively.

Conclusion: The results showed relationship between the presence of virulence factors and antimicrobial resistance. These results can be used for further studies on STEC as an emerging foodborne pathogen.

Keywords: Escherichia coli, milk products, molecular characterization.


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