Vet World Vol.10 October-2017 Article-20
Research Article
Veterinary World, 10(10): 1286-1291
https://doi.org/10.14202/vetworld.2017.1286-1291
Survivin expression in canine spontaneous cutaneous and subcutaneous tumors and its prognostic importance
2. Department of Biotechnology, Institute of Animal Health and Veterinary Biologicals, Veterinary College, Karnataka Veterinary Animal and Fisheries Sciences University, Bengaluru, Karnataka, India.
3. Department of Veterinary Surgery and Radiology, Veterinary College, Karnataka Veterinary Animal and Fisheries Sciences University, Bengaluru, Karnataka, India.
4. Department of Veterinary Medicine, Veterinary College, Karnataka Veterinary Animal and Fisheries Sciences University, Bengaluru, Karnataka, India.
Background and Aim: The present study was carried out to know the expression level of survivin, an inhibitor of apoptosis protein with an objective to determine its prognostic importance in cutaneous and subcutaneous tissue tumors of dogs.
Materials and Methods: Forty cases of canine cutaneous and subcutaneous tissue tumors on histopathological examination revealed various round cell, epithelial, and mesenchymal cell tumors. Survivin gene expression was detected in all tumors tested by TaqMan real-time polymerase chain reaction assay by comparative cycle threshold method.
Results: The mean survivin gene expression value of benign tumors was 0.94±0.63 folds and that of malignant tumors was 18.87±5.30 folds. Postsurgical follow up of 30 malignant tumor cases revealed death in 8, recurrence in 7, and neoplastic free alive status in 15 dogs with mean survivin fold difference values of 48.49±12.39, 14.63±6.37, and 5.034±2.27, respectively. The mean survivin gene expression value was significantly higher in malignant (30 cases, 18.87±5.30) compared to benign tumors (10 cases, 0.94±0.63), and it varied between various postsurgical follow-up groups (p<0.05). Survival analysis, using survivin gene expression median cutoff value of 3.74 in 30 malignant tumors, was performed to predict probable survival period in malignant cutaneous and subcutaneous tumors of dogs.
Conclusion: Results of the present study indicated that the expression of survivin in canine cutaneous and subcutaneous tumors has prognostic value, and survivin expression greater than median cutoff value of 3.74 has a poor prognosis. Keywords: cutaneous and subcutaneous tumors, prognostic value, quantitative real-time polymerase chain reaction, survivin.
Keywords: cutaneous and subcutaneous tumors, prognostic value, quantitative real-time polymerase chain reaction, survivin.
How to cite this article: Kavya N, Rao S, Sathyanarayana ML, Narayanaswamy HD, Byregowda SM, Ranganath L, Kamaran A, Purushotham KM, Kishore TK (2017) Survivin expression in canine spontaneous cutaneous and subcutaneous tumors and its prognostic importance, Veterinary World, 10(10): 1286-1291.
Received: 07-06-2017 Accepted: 21-09-2017 Published online: 31-10-2017
Corresponding author: N. Kavya E-mail: kavyavetico@gmail.com
DOI: 10.14202/vetworld.2017.1286-1291
Copyright: Kavya, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.