Open Access
Research (Published online: 06-09-2017)
4. Detection of Toxoplasma gondii in cat's internal organs by immunohistochemistry methods labeled with-[strept] avidin-biotin
Muhammad Hanafiah, Raden Wisnu Nurcahyo, Rika Yuniar Siregar, Joko Prastowo, Sri Hartati, Bambang Sutrisno and Dwinna Aliza
Veterinary World, 10(9): 1035-1039

Muhammad Hanafiah: Parasitology Laboratory, Faculty of Veterinary Medicine, Syiah Kuala University, Banda Aceh, Indonesia.
Raden Wisnu Nurcahyo: Department of Parasitology, Faculty of Veterinary Medicine, Gadjah Mada University, Yogyakarta, Indonesia.
Rika Yuniar Siregar: Veterinary Public Health of Laboratory, Diseases Investigation Center, Wates, Yogyakarta, Indonesia.
Joko Prastowo: Department of Parasitology, Faculty of Veterinary Medicine, Gadjah Mada University, Yogyakarta, Indonesia.
Sri Hartati: Department of Clinic, Faculty of Veterinary Medicine, Gadjah Mada University, Yogyakarta, Indonesia.
Bambang Sutrisno: Department of Pathology, FKH UGM, Sekip Unit II Yogyakarta 55281, Yogyakarta, Indonesia.
Dwinna Aliza: Pathology Laboratory, Faculty of Veterinary Medicine, Syiah Kuala University Banda Aceh, Indonesia.

doi: 10.14202/vetworld.2017.1035-1039

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Article history: Received: 22-03-2017, Accepted: 03-08-2017, Published online: 06-09-2017

Corresponding author: Muhammad Hanafiah


Citation: Hanafiah M, Nurcahyo RW, Siregar RY, Prastowo J, Hartati S, Sutrisno B, Aliza D (2017) Detection of Toxoplasma gondii in cat's internal organs by immunohistochemistry methods labeled with-[strept] avidin-biotin, Veterinary World, 10(9): 1035-1039.

Aim: The aims of the study are to detect the presence of Toxoplasma gondii antigen and to determine its distribution location in several organs of domestic cat using immunohistochemistry (IHC) method with Labeled-[Strept] Avidin-Biotin (LAB-SA).

Materials and Methods: Four domestic cats aged 1-2 years were used as sample in this research. The sample divided into two groups with two cats each. Cats in Group I were positive Toxoplasma based on serologically screening test, while cats in Group II were orally infected with 1x106 Toxoplasma oocyst. All samples then necropsied, and the organs including brain, liver, kidney, duodenum, jejunum, ileum, lungs, and spleen were collected for IHC method with LAB-SA.

Result: The result showed that Toxoplasma antigens were detected in ileum of both serologically positive domestic cat and the experimentally infected cats. Toxoplasma was also observed in kidney of serologically positive domestic cat. In the serologically positive domestic cat, necrotic lesions were found on ileum, kidney, and liver, whereas in experimentally infected cat, the lesion was only found on ileum.

Conclusion: The presence of Toxoplasma antigen is successfully detected in several organs of domestic cat using IHC method with the LAB-SA.

Keywords: cat, immunohistochemistry, labeled-[strept] avidin-biotin, Toxoplasma gondii.


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