Open Access
Research (Published online: 11-09-2017)
6. Hydrophilic nanosilica as a new larvicidal and molluscicidal agent for controlling of major infectious diseases in Egypt
Marwa M. Attia, Soliman M. Soliman and Mahmoud A. Khalf
Veterinary World, 10(9): 1046-1051

Marwa M. Attia: Department of Parasitology, Cairo University, Giza, P.O. Box 12211, Egypt.
Soliman M. Soliman: Department of Medicine and Infectious Diseases, Cairo University, Giza, P.O. Box 12211, Egypt.
Mahmoud A. Khalf: Department of Veterinary Hygiene and Management, Faculty of Veterinary Medicine, Cairo University, Giza, P.O. Box 12211, Egypt.

doi: 10.14202/vetworld.2017.1046-1051

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Article history: Received: 19-05-2017, Accepted: 10-08-2017, Published online: 11-09-2017

Corresponding author: Marwa M. Attia


Citation: Attia MM, Soliman SM, Khalf MA (2017) Hydrophilic nanosilica as a new larvicidal and molluscicidal agent for controlling of major infectious diseases in Egypt, Veterinary World, 10(9): 1046-1051.

Aim: This research was conducted to evaluate the molluscicidal and mosquitocidal efficacy of silica nanoparticles in the eradication of the larvae and pupa of malaria and filariasis vector as well as vectors of rift-valley fever virus (Culex pipiens); Schistosoma mansoni vector (Biomphlaria alexandrina (snail and egg masses)).

Materials and Methods: Hydrophilic nanosilica particles (NSPs) were characterized using transmission electron microscope during the preliminary part of the study; the stages were exposed to upgrade concentrations of NSP from 50 to 1200 ppm each for 24-36 h exposure time. The highly effective concentrations were re-evaluated at lower exposure time as 3, 6, and 12 h.

Results: Lethal concentration (LC50) and LC90 versus mosquito larvae were (350 ppm/24 h and 1400 ppm/24 h, respectively). C. pipiens pupae proved slight high tolerance versus the effect of these nanoparticles as the two previous doses increased to 680 ppm/6 h and 1300 ppm/24 h. The LC50 and LC90 versus B. alexandrina were increased to 590 ppm/6 h and 980 ppm/48 h, respectively. Moreover, the embryonated snail egg masses appear more susceptible to the toxic effect of these nanoparticles than the non-embryonated eggs as the LC50 and LC90 were increased to 1450 ppm/12 h and 1250 ppm/48 h, respectively, for embryonated eggs, and it was 1400 ppm/24 h and 1890 ppm/48 h, respectively, for non-embryonated one.

Conclusion: The results open a new field for controlling the infectious diseases through eradication of their vectors by the way that avoids the resistance recorded from the successive chemical application in this field.

Keywords: Biomphalaria alexandrina, Culex pipiens, Egypt, nanosilica, rift valley fever, schistosomiasis.


1. WHO. (2005) Guidelines for Laboratory and Field Testing of Mosquito Larvicides WHO/CDS/WHOPES/GCDPP/2005.13. WHO, Geneva.

2. Azari-Hamidian, S., Yaghoobi-Ershadi, M.R., Javadian, E., Abai, M.R., Mobedi, I., Linton, Y.M. and Harbach, R.E. (2009) Distribution and ecology of mosquitoes in a focus of dirofilariasis in Northwestern Iran, with the first finding of filarial larvae in naturally infected local mosquitoes. Med. Vet. Entomol., 23(2): 111-121. [Crossref] [PubMed]

3. King, C.H. and Dangerfield-Cha, M. (2008) The unacknowledged impact of chronic schistosomiasis. Chrsonic Illn., 4: 65-79. [Crossref] [PubMed]

4. DeJong, R.J., Morgan, J.A., Paraense, W.L., Pointier, J.P., Amarista, M., Ayeh-Kumi P.F., Babiker, A., Barbosa, C.S., Bremond, P., Pedro Canese, A., de Souza, C.P., Dominguez, C., File, S., Gutierrez, A., Incani, R.N., Kawano, T., Kazibwe, F., Kpikpi, J., Lwambo. N.J., Mimpfoundi, R., Njiokou, F., Noel Poda, J., Sene, M., Velasquez, L.E., Yong. M., Adema, C.M., Hofkin, B.V., Mkoji, G.M. and Loker, E.S. (2001) Evolutionary relationships and biogeography of (Gasteropoda: Planorbidae) with implications regarding its role as host of the human blood fluke, Schistosoma mansoni. Mol. Biol. Evol., 18: 2225-2239. [Crossref] [PubMed]

5. Davis, A. (1996) Schistosomiasis. In: Cook, G.C., editor. Manson's Tropical Diseases. 20th ed. W.B. Saunders Company, Philadelphia, PA. p1414-1456.

6. WHO. (1985) The Control of Schistosomiasis. Report of a WHO Expert Committee. Technical Report Series No. 728. WHO, Geneva, Switzerland.

7. Pretty, J., Angus, C., Bain, M., Barton, J., Gladwell, V., Hine, R., Pilgrim, S., Cock, S.S. and Sellens, M. (2009) Nature, Childhood, Health and Life Pathways, Interdisciplinary Centre for Environment and Society Occasional Paper 2009-02. University of Essex, UK.

8. Jones, N., Ray, B., Ranjit, K.T. and Manna, A.C. (2008) Antibacterial activity of ZnO nanoparticle suspensions on a broad spectrum of microorganisms. FEMS Microbiol. Lett., 279: 71-76. [Crossref] [PubMed]

9. IARC. (2007) International Agency for Research on Cancer (IARC). Summaries and Evaluations-SILICA. 2 June; 2007.

10. Barik, T.K., Kamaraju, R. and Gowswami, R. (2012) Silica nanoparticle: A potential new insecticide for mosquito vector control. Parasitol. Res., 111: 1075-1083. [Crossref] [PubMed]

11. Marimuthu, S., Rahuman, A.A., Rajakumar, G., Kumar, T.S., Kirthi, A.V., Jayaseelan, C., Bagavan, A., Zahir, A.A., Elango, G. and Kamaraj, C. (2011) Evaluation of green synthesized green silver nanoparticles against parasites. Parasitol. Res., 108: 1541-1549. [Crossref] [PubMed]

12. Kamaraj, C., Bagavan, A., Elango, G., Zahir, A.A., Rajakumar, G. and Marimuthu, S. (2011) Larvicidal activity of medicinal plant extracts against Anopheles subpictus and Culex tritaeniorhynchus. Indian J. Med. Res., 134: 101-106. [PubMed] [PMC]

13. Chrsistensen, N.O., Mutani, A. and Frandsen, F. (1983) A review of the biology and transmission ecology of African bovine species of the genus Schistosoma. Z. Parasitenkd., 69(5): 551-570. [PubMed]

14. Chrsistensen, N.O. and Frandsen, A. (1985) An introduction to the taxonomy, morphology, biology and transmission ecology of species of the genus Schistosoma causing human African schistosomiasis. Danish Bilharsiasis Laboratory, Denmark. p33.

15. WHO. (1996) Report of the WHO Informal Consultation on the Evaluation on the Testing of Insecticides CTD/WHO PES/IC/96.1:69. WHO, Geneva.

16. Rawi, S.M., Al-Hazmi, M. and Al-Nassr, F.S. (2011) Comparative study of the molluscicidal activity of some plant extracts on the snail vector of Schistosoma mansoni, Biomphalaria alexandrina. Int. J. Zool. Res., 7: 169-189. [Crossref]

17. Lima, J.B., Da-Cunha, M.P., Da Silva, R. C., Galardo, A.K., Soares Sda, S., Braga, I.A., Ramos, R.P. and Valle, D. (2003) Resistance of Aedes aegypti to organophosphates in several municipalities in the state of Rio de Janeiro and Espirito Santo, Brazil. Am. J. Trop. Med. Hyg., 68(3): 329-333. [PubMed]

18. Fahmy, S.R., Abdel-Ghaffar, F., Bakry, F.A. and Sayed, D.A. (2014) Ecotoxicological effect of sublethal exposure to zinc oxide nanoparticles on freshwater snail Biomphalaria alexandrina. Arch. Environ. Contam. Toxicol., 67: 192-202. [Crossref] [PubMed]

19. Finney, D.J. (1971) Probit Analysis. Cambridge University Press, Cambridge. p333.

20. Chuiko, A.A. (2003) Medical Chemistry and Clinical Application of Silicon Dioxide. Nukova Dumka, Kiev, Russia. p143-157.

21. Tiwari, D.K. and Behari, J. (2009) Biocidal nature of treatment of Ag-nanoparticle and ultrasonic irradiation in Escherichia coli dh5. Adv. Biol. Res., 3(3-4): 89-95.

22. Barik, T.K., Sahu, B. and Swain, V. (2008) Nanosilica from medicine to pest control. Parasitol. Res., 103(2): 253-258. [Crossref] [PubMed]

23. Salunkhe, R.B., Patil, S.V., Patil, C.D. and Salunke, B.K. (2011) Larvicidal potential of silver nanoparticles synthesized using fungus Cochliobolus lunatus against Aedes aegypti (Linnaeus, 1762) and Anopheles stephensi Liston (Diptera; Culicidae). Parasitol. Res., 109: 823-831. [Crossref]

24. Patil, C.D., Patil, S.V., Borase, H.P., Salunke, B.K. and Salunkhe, R.B. (2012) Larvicidal activity of silver nanoparticles synthesized using Plumeria rubra plant latex against Aedes aegypti and Anopheles stephensi. Parasitol. Res., 110: 1815-1822. [Crossref] [PubMed]