Vet World Vol.11 April-2018 Article-10
Research Article
Veterinary World, 11(4): 469-473
https://doi.org/10.14202/vetworld.2018.469-473
Characterization of quinolone-resistant Enterobacteriaceae strains isolated from poultry in Western Algeria: First report of qnrS in an Enterobacter cloacae
2. Research Laboratory, Health and Animal Production, Higher National Veterinary School, Algiers, Algeria.
3. Medical Bacteriology Laboratory, Pasteur Institute of Algeria, Algiers, Algeria.
4. Veterinary Sciences Institute, Ibn Khaldoun University, Tiaret, Algeria.
5. Higher School of Food Sciences and Agro-alimentary Industries, Algiers, Algeria.
Background and Aim: Multidrug-resistant (MDR) Enterobacteriaceae have frequently been reported, in both human and veterinary medicine, from different parts of the world as a consequence of antibiotic usage. However, there is a lack of published data regarding antimicrobial resistance in non-Escherichia coli (E. coli) Enterobacteriaceae from animals in Algeria. This study aimed to evaluate the frequency of resistance to antibiotics with a focus on quinolones and to investigate the presence of qnr genes in Enterobacteriaceae of poultry origin.
Materials and Methods: A total of 310 samples of poultry origin were collected from 2010 to 2014 from broiler and layer farms and hatcheries located in different geographic areas of Western Algeria (including Mostaganem, Oran, Mascara, Relizane, Chlef, Tiaret, and Tissemsilt). Antimicrobial susceptibility testing was performed using disc diffusion assay. Polymerase chain reaction and sequencing accomplished the characterization of qnr genes (qnrA, qnrB, and qnrS).
Results: A total of 253 Enterobacteriaceae strains were isolated in this study. These isolates exhibited high levels of resistance to quinolones and other families of antibiotics. All the strains isolated in this study were resistant to at least one antibiotic. Among them, 233 (92.09%) were considered MDR. Among the 18 randomly selected nalidixic acid (NA)- resistant Enterobacteriaceae isolates, one E. coli and one Enterobacter cloacae were carrying qnrS1. By contrast, qnrA and qnrB were not detected in this study.
Conclusion: This is the first report on the identification of the qnrS gene in E. cloacae isolated from animal source in Algeria. Further studies have to be conducted to determine the real prevalence of qnr genes. Keywords: Algeria, antimicrobial resistance, Enterobacteriaceae, qnrS1.
Keywords: Algeria, antimicrobial resistance, Enterobacteriaceae, qnrS1.
How to cite this article: Benameur Q, Tali-Maamar H, Assaous F, Guettou B, Boutaiba Benklaouz M, Rahal K, Ben-Mahdi MH (2018) Characterization of quinolone-resistant Enterobacteriaceae strains isolated from poultry in Western Algeria: First report of qnrS in an Enterobacter cloacae, Veterinary World, 11(4): 469-473.
Received: 21-12-2017 Accepted: 06-03-2018 Published online: 12-04-2018
Corresponding author: Qada Benameur E-mail: qada.benameur@univ-mosta.dz
DOI: 10.14202/vetworld.2018.469-473
Copyright: Benameur, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.