Open Access
Research (Published online: 18-04-2018)
15. Viability test of fish scale collagen (Oshpronemus gouramy) on baby hamster kidney fibroblasts-21 fibroblast cell culture
Chiquita Prahasanti, Denny Tri Wulandari and Noer Ulfa
Veterinary World, 11(4): 506-510

Chiquita Prahasanti: Department of Periodontic, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia.
Denny Tri Wulandari: Department of Periodontic, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia.
Noer Ulfa: Department of Periodontic, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia.

doi: 10.14202/vetworld.2018.506-510

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Article history: Received: 10-11-2017, Accepted: 06-03-2018, Published online: 18-04-2018

Corresponding author: Chiquita Prahasanti


Citation: Prahasanti C, Wulandari DT, Ulfa N (2018) Viability test of fish scale collagen (Oshpronemus gouramy) on baby hamster kidney fibroblasts-21 fibroblast cell culture, Veterinary World, 11(4): 506-510.

Aim: This study aims to examine the toxicity of collagen extracted from gouramy fish scales (Oshpronemus gouramy) by evaluating its viability against baby hamster kidney fibroblasts-21.

Materials and Methods: Collagen was extracted from gouramy fish scales (O. gouramy) with 6% acetic acid. Its results were analyzed using Fourier-transform infrared spectroscopy and freeze-dried technique. Its morphology then was analyzed with scanning electron microscope. Afterward, 3-(4.5-dimethylthiazole-2-yl)2.5-diphenyl tetrazolium bromide assay was conducted to compare cells with and without fish scale collagen treatment.

Results: Collagen extracted from gouramy fish scales had no influence statistically on cultured fibroblast cells with a statistical significance (2-tailed) value of 0.754 (p>00025).

Conclusion: Collagen extracted from gouramy fish scales has high viability against BHK21 fibroblast cells.

Keywords: bone graft, collagen, gouramy fish scale, viability, 3-(4.5-dimethylthiazole-2-yl)2.5-diphenyl tetrazolium bromide.


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