Article history: Received: 19-03-2018, Accepted: 14-06-2018, Published online: 02-08-2018
Corresponding author: Manisha Punia
E-mail: firstname.lastname@example.orgCitation: Punia M, Kumar A, Charaya G, Kumar T (2018) Pathogens isolated from clinical cases of urinary tract infection in dogs and their antibiogram, Veterinary World, 11(8): 1037-1042.
Aim: This study aims to determine the etiology of urinary tract infection (UTI) in dogs and to develop an antibiogram of organisms isolated.
Materials and Methods: Urine samples were collected either through catheterization or cystocentesis from 35 dogs suspected of UTI admitted to VCC, LUVAS, Hisar. Bacteria were identified on the basis of cultural characteristics in 22 samples, and all the isolates were subjected to in vitro antimicrobial sensitivity testing.
Results: The urine samples found positive for bacteria yielded pure colony growth in 77.27% and mixed growth in 22.73% samples, respectively. Escherichia coli (29.62%) and Streptococcus spp. (29.62%) were the most prevalent microorganisms followed by Staphylococcus spp. (22.22%), Klebsiella spp. (11.11%), Pseudomonas spp. (3.7%), and Bacillus spp. (3.7%). Overall, maximum sensitivity of isolates was found toward ceftriaxone/tazobactam (88.88%) and least toward amoxicillin and cloxacillin (29.62%).
Conclusion: E. coli and Streptococcus spp. were the most predominant bacteria isolated from UTI affected dogs. In vitro sensitivity revealed a significant proportion of bacteria to be multidrug resistant.
Keywords: antibiogram, multidrug resistance, Escherichia coli, Staphylococci, Streptococci.
1. Ling, G.V. (1984) Therapeutic strategies involving antimicrobial treatment of the canine urinary tract. J. Am. Vet. Med. Assoc., 185(10): 1162-1164. [PubMed]
2. Litster, A., Moss, S.M., Honnery, M., Rees, B. and Trott, D.J. (2007) Prevalence of bacterial species in cats with clinical signs of lower urinary tract disease: Recognition of Staphylococcus felis as a possible feline urinary tract pathogen. Vet. Microbiol., 121: 182-188. [Crossref]
4. Dorsch, R., von Vopelius-Feldt, C., Wolf, G., Straubinger, R.K. and Hartmann, K. (2015) Feline urinary tract pathogens: Prevalence of bacterial species and antimicrobial resistance over a 10-year period. Vet. Rec., 176: 201. [Crossref]
5. Feria, C., Correia, J., Machado, J., Vidal, R. and Goncalves, J. (2000) Urinary tract infection in dogs. Analysis of 419 urocultures carried out in Portugal. Adv. Exp. Me. Biol., 485: 301-304. [Crossref] [PubMed]
6. Ball, K.R., Rubin, J.E., Chirino-Trejo, M. and Dowling, P.M. (2008) Antimicrobial resistance and prevalence of canine uropathogens at the Western College of Veterinary Medicine Veterinary Teaching Hospital, 2002-2007. Can. Vet. J., 49: 985-990. [PubMed] [PMC]
8. Ewers, C., Grobbel, M., Bethe, A., Wieler, L.H. and Guenther, S. (2011) Extended-spectrum beta-lactamases producing gram-negative bacteria in companion animals: Action is clearly warranted! Berl. Munch. Tierarztl. Wochenschr., 124(3-4): 94-101. [PubMed]
11. Kogika, M.M., Fortunato,V.A.B., Mamizuka, E.M., Hagiwara, M.K., Pavan, M.F.B. and Grosso, S.N.A. (1995) Etiologic study of urinary tract infection in dogs. Braz. J. Vet. Res. Anim. Sci., 32: 31-36. [Crossref]
12. Hariharan, H., Brathwaite-Sylvester, E., Belmar, V.M. and Sharma, R. (2016) Bacterial isolates from urinary tract infection in dogs in Grenada, and their antibiotic susceptibility. Open J. Vet. Med., 6: 85-88. [Crossref]
13. Marques, C., Gama, L.T., Belas, A., Bergstrom, K., Beurlet, S., Marchal, A.B., Broens, E.M., Costa, M., Criel, D., Damborg, P., Van Dijk, M.A.M., Van Dongen, A.M.,Dorsch, R., Espada, C.M., Gerber, B., Konstantinou, M.K., Loncaric, I., Mion, D., Misic, D., Movilla, R., Overesch, G., Perreten, V., Roura, X., Steenbergen, J., Timofte, D., Wolf, G., Zanoni, R.G., Schmitt, S., Guardabassi, L. and Pomba, C. (2016) European multicenter study on antimicrobial resistance in bacteria isolated from companion animal urinary tract infections. BMC Vet. Res., 12: 213. [Crossref] [PubMed] [PMC]
14. Windahl, U., Holst, B.S., Nyman, A., Gronlund, U. and Bengtsson, B. (2014) Characterisation of bacterial growth and antimicrobial susceptibility patterns in canine urinary tract infections. BMC Vet. Res., 10: 217. [Crossref] [PubMed] [PMC]
15. McMeekin, C.H., Hill, K.E., Gibson, I.R., Bridges, J.P. and Benschop, J. (2016) Antimicrobial resistance patterns of bacteria isolated from canine urinary samples submitted to a New Zealand veterinary diagnostic laboratory between 2005-2012. New Zealand Vet. J., 65(2): 99-104. [Crossref] [PubMed]
16. Moyaert, H., Morrissey, I., de Jong, A., El Garch, F., Klein, U., Ludwig, C., Thiry, J. and Youala, M. (2017) Antimicrobial susceptibility monitoring of bacterial pathogens isolated from urinary tract infections in dogs and cats across Europe: Com path results. Microb. Drug Resist., 23(3): 391-403. [Crossref] [PubMed]17. Chang, S.K., Lo, D.Y., Wei, H.W. and Kuo, H.C. (2015). Antimicrobial resistance of Escherichia coli isolates from canine urinary tract infections. J. Vet. Med. Sci., 77(1): 59-65. [Crossref] [PubMed] [PMC]