Vet World   Vol.11   February-2018  Article-11

Effect of lipopolysaccharide derived from surabaya isolates of Actinobacillus actinomycetemcomitans on alveolar bone destruction

Background and Aim: The aim of the study was to identify and evaluate the effect of LPS derived from local isolates (A. actinomycetemcomitans) on the destruction of alveolar bone by means of several biomarkers, including; the number of osteoblasts and osteoclasts, the expression of IL-6, matrix metallopeptidase 1 (MMP-1), and receptor activator of nuclear factor kappa-? ligand (RANKL).

Materials and Methods: The isolation of LPS from A. actinomycetemcomitans was calculated using phenol, while purification was performed using Sephadex C-18 column chromatography. 40 Wistar rats were divided into four groups of 10. Each treatment was divided into two groups which were 0.9% NaCl and LPS induced for 7 and 14 days, respectively. Gingival and alveolar bones were further introduced into the induction area, followed by the measuring of osteoblast and osteoclast with hematoxylin-eosin staining, IL-6, MMP-1 and RANKL expression with immunohistochemical.

Results: Reduced numbers of osteoblasts at the 7th and 14th day of treatment were detected, while those of osteoclasts increased. There was an increased expression of IL-6, MMP-1, and RANKL in the 7th and 14th-day treatment group. Treatment of LPS from A. actinomycetemcomitans over 7 and 14 days resulted in damage to periodontal tissue and alveolar bone in Wistar rats.

Conclusion: LPS of A. actinomycetemcomitans administration for 7 and 14 days causes periodontal and alveolar tissue destruction in Wistar rats. Keywords: Actinobacillus actinomycetemcomitans local isolate, lipopolysaccharide, interleukineIL-6, matrix metallopeptidase-1, receptor activator of nuclear factor kappa-B, receptor activator of nuclear factor kappa-B ligand.

Keywords: Actinobacillus actinomycetemcomitans local isolate, lipopolysaccharide, interleukineIL-6, matrix metallopeptidase-1, receptor activator of nuclear factor kappa-B, receptor activator of nuclear factor kappa-B ligand.

How to cite this article: Ridwan RD, Sidarningsih, Kusumaningsih T, Salim S (2018) Effect of lipopolysaccharide derived from surabaya isolates of Actinobacillus actinomycetemcomitans on alveolar bone destruction, Veterinary World, 11(2): 161-166.

Received: 11-10-2017  Accepted: 03-01-2018     Published online: 10-02-2018

Corresponding author: Rini Devijanti Ridwan   E-mail: rini-d-r@fkg.unair.ac.id

DOI: 10.14202/vetworld.2018.161-166

Copyright: Ridwan, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.