Identification and antibiogram pattern of Bacillus cereus from the milk and milk products in and around Jammu region

Vet World Vol.11 February-2018 Article-16

Research Article

Veterinary World, 11(2): 186-191

https://doi.org/10.14202/vetworld.2018.186-191

Umar Yusuf¹, S. K. Kotwal¹, Sanjolly Gupta¹, and Touqeer Ahmed²

1. Division of Veterinary Public Health and Epidemiology, F.V.Sc. & A.H, SKUAST-J, Jammu, Jammu and Kashmir, India.
2. Division of Animal Reproduction Gynaecology and Obstetrics, F.V.Sc. & A.H, SKUAST-K, Srinagar, Jammu and Kashmir, India.

Background and Aim: The aims of the present study were to assess the prevalence, identification, and antibiogram pattern of Bacillus cereus from 215 samples of different milk and milk products in and around Jammu region.

Materials and Methods: In the present study, 215 samples of milk, rasgulla, burfi, rasmalai, kalaari, paneer, ice cream, and pastry were collected and analyzed for the isolation of the B. cereus using PEMBA, and antibiogram pattern was observed for all the milk and milk products.

Results: B. cereus was detected in 61/215 samples with an overall prevalence of 28.37%. Biotyping revealed predominantly 5, 7, and 2 biotypes in raw milk. Burfi and ice cream revealed 2, 3, 5, and 7 biotypes. Rasgulla had 2, 3, and 5 biotypes; paneer and rasmalai had biotypes 2 and 5, while kalaari revealed biotype 5. Antibiogram pattern revealed that isolates were highly sensitive to gentamicin (100%), intermediate to ampicillin (40.98%), tetracycline (31.14%), erythromycin (29.50%), and amoxicillin (26.22%), and high resistance against penicillin G (100%). Adulteration of starch was detected in 16.66 % raw milk samples. All starch positive samples were positive for B. cereus. However, 12 starch negative samples also yielded B. cereus.

Conclusion: From this study, it was concluded that highest prevalence of B. cereus was found in ice cream. Several isolates of B. cereus showed toxigenic activity, so the presence of B. cereus in milk and milk products may be of public health hazard. The antibiogram pattern of B. cereus isolates showed sensitivity to gentamicin, ciprofloxacin, chloramphenicol, streptomycin, and resistance to penicillin-G and cephalexin. The presence of B. cereus in milk and milk products showed a strong association besides establishing the fact that starch adulteration can be indicative of the presence of B. cereus. Keywords: Antibiogram, Bacillus cereus, milk, milk products, prevalence.

Keywords: Antibiogram, Bacillus cereus, milk, milk products, prevalence.

How to cite this article: Yusuf U, Kotwal SK, Gupta S, Ahmed T (2018) Identification and antibiogram pattern of Bacillus cereus from the milk and milk products in and around Jammu region, Veterinary World, 11(2): 186-191.

Received: 19-09-2017 Accepted: 10-01-2018 Published online: 14-02-2018

Corresponding author: Sanjolly Gupta E-mail: gupta.sanjolly@gmail.com

DOI: 10.14202/vetworld.2018.186-191

Copyright: Yusuf, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.