Article history: Received: 23-10-2017, Accepted: 16-01-2018, Published online: 20-02-2018
Corresponding author: G. N. Mahardika
E-mail: firstname.lastname@example.orgCitation: Suartha IN, Suartini GAA, Wirata IW, Dewi NMARK, Putra GNN, Kencana GAY, Mahardika GN (2018) Intranasal administration of inactivated avian influenza virus of H5N1 subtype vaccine-induced systemic immune response in chicken and mice, Veterinary World, 11(2): 221-226.
Aim: The need for non-parenteral administration of inactivated avian influenza virus of H5N1 subtype (AIV-H5N1) vaccine is paramount. Here, we provide preliminary data on the immune response of chicken and mice after intranasal administration of AIV-H5N1-inactivated vaccine with ISCOMS, Inmunair (INM), and combined ISCOMS and INM as an adjuvant.
Materials and Methods: The AIV isolate of A/Chicken/Denpasar/01/2004 (H5N1) was cultivated in specific pathogen-free chicken eggs and inactivated with formaldehyde. The vaccine preparation was added with those adjuvants for intranasal administration and aluminum hydroxide for subcutaneous injection. The chicken and mouse were vaccinated at the age of 3 weeks or 1 month and repeated 2 weeks thereafter. In one experiment, chicken was injected with Newcastle disease virus (NDV) at the same time with AIV vaccine. The sera were collected at one (serum 1) and 2 w (serum 2) after booster vaccination. The anti-AIV-H5 and NDV antibodies in chicken sera were detected using hemagglutination inhibition (HI) assay. Mouse IgG anti-AIV-H5N1 antibody was detected using ELISA.
Results: The result shows that the geometric mean titers (GMTs) of chicken sera of intranasal vaccinated with inactivated AIV-H5N1 vaccine with mixed ISCOM- INM as adjuvant were <20.0 and 22.7 unit HI-unit (HIU) in serum 1 and serum 2, respectively. The GMTs of the positive control group were 23.7 and 25.7 HIU in serum 1 and serum 2, respectively. The result of the second experiment shows that IgG anti-AIV-H5N1 was detected in mouse sera. In the third experiment, the GMTs of anti-NDV in chicken vaccinated subsequently with inactivated NDV vaccine and AIV-H5N1 with mixed ISCOMS-INM administrated intranasally and aluminum hydroxide adjuvant administrated through subcutaneous injection as well as positive control group receiving NDV vaccine only were 28.0, 28.0, and 27.4 HIU in serum 1 while were 29.6, 29.2, and 28.2 HIU in serum 2, respectively.
Conclusion: Intranasal administration of inactivated AIV-H5N1 vaccine-induced a systemic immune response in chicken and mice after adding ISCOMS and/or INM as adjuvants. The adjuvant and the intranasal administration caused no immunosuppressive effect on the chicken immune response to NDV vaccine.
Keywords: inactivated vaccine, influenza-H5N1, ISCOMS, Inmunair, intranasal.
3. WHO. (2016) Cumulative Number of Confirmed Human Cases for Avian Influenza A(H5N1) Reported to WHO, 2003-2015, as of December 19, 2016. WHO, Rome. Available from: http://www.who.int/influenza/human_animal_interface/2016_12_19_tableH5N1corrected.pdf?ua=1. [Last accessed on 30-01-2017].
4. Edenborough, K.M., Lowther, S., Laurie, K., Yamada, M., Long, F., Bingham, J., Payne, J., Harper, J., Haining, J., Arkinstall, R., Gilbertson, B., Middleton, D. and Brown, L.E. (2015) Predicting disease severity and viral spread of H5N1 influenza virus in ferrets in the context of natural exposure routes. J. Virol., 90: 1888-1897. [Crossref] [PubMed] [PMC]
5. Zaman, M., Gasimov, V., Oner, A.F., Dogan, N., Adisasmito, W., Coker, R., Bamgboye, E.L., Chan, P.K., Hanshaoworakul, W., Lee, N., Phommasack, B., Touch, S., Tsang, O., Swenson, A., Toovey, S. and Dreyer, N.A. (2014) Recognizing true H5N1 infections in humans during confirmed outbreaks. J. Infect. Dev. Ctries, 8: 202-207. [Crossref] [PubMed]
6. Swayne, D.E., Spackman, E. and Pantin-Jackwood, M. (2014) Success factors for avian influenza vaccine use in poultry and potential impact at the wild bird-agricultural interface. Ecohealth, 11: 94-108. [Crossref] [PubMed]
7. Tizard, I. (2013) Veterinary Immunology. 9th ed. Elsevier Saunders, St. Louis - Missouri.
9. Morein, B. (1990) Iscoms. Vet. Microbiol., 23: 79-84. [Crossref]
10. Behboudi, S., Morein, B. and Ronnberg, B. (1995) Isolation and quantification of Quillaja saponaria Molina saponins and lipids in iscom-matrix and iscoms. Vaccine, 13: 1690-1696. [Crossref]
12. Cibulski, S.P., Mourglia-Ettlin, G., Teixeira, T.F., Quirici, L., Roehe, P.M., Ferreira, F. and Silveira, F. (2016) Novel ISCOMs from Quillaja brasiliensis saponins induce mucosal and systemic antibody production, T-cell responses and improved antigen uptake. Vaccine, 34: 1162-1171. [Crossref] [PubMed]
13. Sjolander, A., van't Land, B. and Bengtsson, L.K. (1997) Iscoms containing purified Quillaja saponins upregulate both Th1-like and Th2-like immune responses. Cell Immunol., 177: 69-76. [Crossref] [PubMed]
14. Hecker, Y.P., Canton, G., Regidor-Cerrillo, J., Chianini, F., Morrell, E., Lischinsky, L., Ortega-Mora, L.M., Innes, E.A., Odeon, A., Campero, C.M. and Moore, D.P. (2015) Cell mediated immune responses in the placenta following challenge of vaccinated pregnant heifers with Neospora caninum. Vet. Parasitol., 214: 247-254. [Crossref] [PubMed]
16. Abel, L.C., Chen, S., Ricca, L.G., Martins, M.F., Garcia, M., Ananias, R.Z., Mussalem, J.S., Squaiella, C.C., Shaw, R.J. and Longo-Maugeri, I.M. (2009) Adjuvant effect of LPS and killed Propionibacterium acnes on the development of experimental gastrointestinal nematode infestation in sheep. Parasite Immunol., 31: 604-612. [Crossref] [PubMed]
17. Morrison, D.C. and Ryan, J.L. (1979) Bacterial endotoxins and host immune responses. Adv. Immunol., 28: 293-450. [Crossref]
18. Gambero, M., Teixeira, D., Butin, L., Ishimura, M.E., Mariano, M., Popi, A.F. and Longo-Maugeri, I.M. (2016) Propionibacterium acnes induces an adjuvant effect in B-1 cells and affects their phagocyte differentiation via a TLR2-mediated mechanism. Immunobiology, 221: 1001-1011. [Crossref] [PubMed]
19. Mussalem, J.S., Squaiella-Baptistao, C.C., Teixeira, D., Yendo, T.M., Thies, F.G., Popi, A.F., Mariano, M. and Longo-Maugeri, I. (2012) Adjuvant effect of killed Propionibacterium acnes on mouse peritoneal B-1 lymphocytes and their early phagocyte differentiation. PLoS One, 7: e33955. [Crossref]
20. FAO (2017) Avian influenza. In: Manual of Diagnostic Tests and Vaccines for Terrestrial Animals. FAO, Rome. p1-23. Available from: http://www.oie.int/international-standard-setting/terrestrial-manual/access-online/. [Last accessed on 23-08-2017].
22. Tumpey, T.M., Renshaw, M., Clements, J.D. and Katz, J.M. (2001) Mucosal delivery of inactivated influenza vaccine induces B-cell-dependent heterosubtypic cross-protection against lethal influenza A H5N1 virus infection. J. Virol., 75: 5141-5150. [Crossref]
23. Lau, Y.F., Santos, C., Torres-Velez, F.J. and Subbarao, K. (2011) The magnitude of local immunity in the lungs of mice induced by live attenuated influenza vaccines is determined by local viral replication and induction of cytokines. J. Virol., 85: 76-85. [Crossref]
24. Asahi-Ozaki, Y., Itamura, S., Ichinohe, T., Strong, P., Tamura, S., Takahashi, H., Sawa, H., Moriyama, M., Tashiro, M., Sata, T., Kurata, T. and Hasegawa, H. (2006) Intranasal administration of adjuvant-combined recombinant influenza virus HA vaccine protects mice from the lethal H5N1 virus infection. Microbes. Infect., 8: 2706-2714. [Crossref] [PubMed]
25. Ichinohe, T., Kawaguchi, A., Tamura, S., Takahashi, H., Sawa, H., Ninomiya, A., Imai, M., Itamura, S., Odagiri, T., Tashiro, M., Chiba, J., Sata, T., Kurata, T. and Hasegawa, H. (2007) Intranasal immunization with H5N1 vaccine plus poly I: Poly C12U, a toll-like receptor agonist, protects mice against homologous and heterologous virus challenge. Microbes. Infect., 9: 1333-1340. [Crossref]26. Trondsen, M,. Aqrawi, L.A., Zhou, F., Pedersen, G., Trieu, M.C., Zhou, P. and Cox, R.J. (2015) Induction of local secretory IgA and multifunctional CD4(+) T-helper cells following intranasal immunization with a H5N1 whole inactivated influenza virus vaccine in BALB/c Mice. Scand. J. Immunol., 81: 305-317. [Crossref]