Open Access
Research (Published online: 28-01-2018)
14. In vitro immunomodulatory potential of Artemisia indica Willd. in chicken lymphocytes
Pushpa Ruwali, Tanuj Kumar Ambwani and Pankaj Gautam
Veterinary World, 11(1): 80-87

Pushpa Ruwali: Department of Biotechnology, Graphic Era University, Dehradun - 248 002, Uttarakhand, India.
Tanuj Kumar Ambwani: Department of Veterinary Physiology and Biochemistry, College of Veterinary & Animal Sciences, G. B. Pant University of Agriculture & Technology, Pantnagar - 263 145; Uttarakhand, India.
Pankaj Gautam: Department of Biotechnology, Graphic Era University, Dehradun - 248 002, Uttarakhand, India.

doi: 10.14202/vetworld.2018.80-87

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Article history: Received: 11-08-2017, Accepted: 12-12-2017, Published online: 28-01-2018

Corresponding author: Tanuj Kumar Ambwani


Citation: Ruwali P, Ambwani TK, Gautam P (2018) In vitro immunomodulatory potential of Artemisia indica Willd. in chicken lymphocytes, Veterinary World, 11(1): 80-87.

Aim: Evaluation of the in vitro immunomodulatory potential of Artemisia indica Willd. methanolic extract in chicken lymphocyte culture system through lymphocyte (B and T cells) proliferation assay, after standardizing the maximum non-cytotoxic dose (MNCD) in chicken lymphocytes.

Materials and Methods: Fresh aerial parts of A. indica Willd. (family: Asteraceae) specimens were collected (altitude 1560 m), gotten authenticated, processed, dried, and Soxhlet extracted to yield methanolic extract (AME). Chicken splenocytes were isolated from spleens collected from healthy birds; lymphocytes were separated by density gradient centrifugation, percentage cell viability determined and final cell count adjusted to 107 cells/ml in RPMI-1640 medium. MNCD of AME in chicken lymphocytes was determined through 3-(4,5-dimethylthiazol-2-y1)-2,5-diphenyltetrazolium bromide dye reduction assay. Immunomodulatory potential of AME was evaluated through lymphocytes proliferation or B and T cells blastogenesis assay in the presence of appropriate mitogens, namely, lipopolysaccharide (LPS) and concanavalin A (Con A), respectively.

Results: Maximum concentration of AME exhibiting 100% cell viability (MNCD) was 200 μg/ml and was selected for further in vitro analysis. The in vitro exposure of chicken lymphocytes to 200 μg/ml dose of AME, resulted in significant (p<0.05) upregulation of 11.76% in B cell proliferation in the presence of B cell mitogen (LPS) and a significant (p<0.05) increase of 12.018% T cells proliferation in the presence of the mitogen (Con A), as compared to the control.

Conclusion: The significant upregulation in the proliferation of two major cell types modulating the immune system is an indication of the immunostimulatory potential of the plant. It would be worthwhile to further evaluate A. indica on relevant immunomodulatory aspects, especially the in vivo studies in a poultry system.

Keywords: Artemisia indica Willd., immunomodulation, in vitro chicken lymphocytes, lymphocyte proliferation assay, plant extract.


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