Open Access
Research (Published online: 12-01-2018)
4. Detection of Ehrlichia, Anaplasma, and Babesia spp. in dogs in Cebu, Philippines
Rochelle Haidee D. Ybanez, Adrian P. Ybanez, Lyra Lee A. Arnado, Laila Monika P. Belarmino, Knowlie Gay F. Malingin, Paul Bien C. Cabilete, Ziggy Ryan O. Amores, Maxfrancis G. Talle, Mingming Liu and Xuenan Xuan
Veterinary World, 11(1): 14-19

Rochelle Haidee D. Ybanez: College of Science, University of the Philippines Cebu, Gorordo Avenue, Lahug, Cebu City 6000, Philippines; National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro City 080-8555, Japan.
Adrian P. Ybanez: College of Science, University of the Philippines Cebu, Gorordo Avenue, Lahug, Cebu City 6000, Philippines; College of Veterinary Medicine at Barili Campus and College of Arts and Sciences at Main Campus, Cebu Technological University, Cor. M. J. Cuenco Ave. and R. Palma St. Cebu City, 6000 Cebu, Philippines; Gullas College of Medicine, Inc., University of the Visayas, Banilad, Mandaue City 6014, Cebu, Philippines.
Lyra Lee A. Arnado: College of Veterinary Medicine, Southwestern University, Villa Aznar, Urgello St., Cebu City 6000, Philippines.
Laila Monika P. Belarmino: College of Veterinary Medicine, Southwestern University, Villa Aznar, Urgello St., Cebu City 6000, Philippines.
Knowlie Gay F. Malingin: College of Veterinary Medicine, Southwestern University, Villa Aznar, Urgello St., Cebu City 6000, Philippines.
Paul Bien C. Cabilete: College of Veterinary Medicine, Southwestern University, Villa Aznar, Urgello St., Cebu City 6000, Philippines.
Ziggy Ryan O. Amores: College of Veterinary Medicine, Southwestern University, Villa Aznar, Urgello St., Cebu City 6000, Philippines.
Maxfrancis G. Talle: College of Veterinary Medicine, Southwestern University, Villa Aznar, Urgello St., Cebu City 6000, Philippines.
Mingming Liu: National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro City 080-8555, Japan.
Xuenan Xuan: National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro City 080-8555, Japan.

doi: 10.14202/vetworld.2018.14-19

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Article history: Received: 06-09-2017, Accepted: 05-12-2017, Published online: 12-01-2018

Corresponding author: Adrian P. Ybanez


Citation: Ybanez RHD, Ybanez AP, Arnado LLA, Belarmino LMP, Malingin KGF, Cabilete PBC, Amores ZRO, Talle MG, Liu M, Xuan X (2018) Detection of Ehrlichia, Anaplasma, and Babesia spp. in dogs in Cebu, Philippines, Veterinary World, 11(1): 14-19.

Background: Ehrlichia, Anaplasma, and Babesia spp. are canine pathogens transmitted by the Rhipicephalus sanguineus tick which can cause varied clinical signs. These pathogens have been investigated in the Philippines, but coinfection has not been reported yet.

Aim: The aim of this study was to evaluate the presence of Ehrlichia/Anaplasma and Babesia spp. in Philippine dogs.

Materials and Methods: A total of 100 dogs from seven different veterinary establishments in Cebu, Philippines, were examined for Ehrlichia/Anaplasma and Babesia spp. infection using peripheral blood smear examination and polymerase chain reaction (PCR). Inclusion criteria included a history or presence of tick infestation, anemia, and/or thrombocytopenia. Clinical signs were recorded. Statistical analyses were performed between PCR positivity and clinical signs and hematological results.

Results: A total of 10 and 18 dogs were found to be positive for Ehrlichia/Anaplasma and Babesia spp., respectively. One animal was PCR positive for both pathogens, which is the first report of coinfection in the country. The most common clinical signs observed include inappetence (89%), lethargy (80%), thrombocytopenia (85%), and anemia (74%). Analyses revealed that inappetence (p=0.044) and weight loss (p=0.028) were found statistically significant with Ehrlichia/Anaplasma infection. Basophil (p=0.001) and eosinophil counts (p=0.000) were also found significantly different between Ehrlichia/Anaplasma spp.-positive and -negative dogs. On the other hand, differential monocyte count (p=0.009) was found significantly different between Babesia spp.-positive and -negative dogs.

Conclusion: The present study showed low infection rates of canine ehrlichiosis/anaplasmosis and babesiosis and provided additional evidence for the presence of the pathogens in the area.

Keywords: Babesia, Cebu, dogs, Ehrlichia/Anaplasma, Philippines.


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