Open Access
Research (Published online: 19-07-2018)
13. Hypoxic preconditioning effect on stromal cells derived factor-1 and C-X-C chemokine receptor type 4 expression in Wistar rat's (Rattus norvegicus) bone marrow mesenchymal stem cells (in vitro study)
Sri Wigati Mardi Mulyani, Diah Savitri Ernawati, Eha Renwi Astuti and Fedik Abdul Rantam
Veterinary World, 11(7): 965-970

Sri Wigati Mardi Mulyani: Department of Dentomaxillofacial Radiology, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia.
Diah Savitri Ernawati: Department of Oral Medicine, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia.
Eha Renwi Astuti: Department of Dentomaxillofacial Radiology, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia.
Fedik Abdul Rantam: Stem Cell Research Center and Development, Airlangga University Surabaya, Indonesia; Lab of Virology and Immunology, Department of Microbiology, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia.

doi: 10.14202/vetworld.2018.965-970

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Article history: Received: 25-01-2018, Accepted: 04-06-2018, Published online: 19-07-2018

Corresponding author: Diah Savitri Ernawati

E-mail: diah-s-e@fkg.unair.ac.id

Citation: Mulyani SWM, Ernawati DS, Astuti ER, Rantam FA (2018) Hypoxic preconditioning effect on stromal cells derived factor-1 and C-X-C chemokine receptor type 4 expression in Wistar rat's (Rattus norvegicus) bone marrow mesenchymal stem cells (in vitro study), Veterinary World, 11(7): 965-970.
Abstract

Aim: To examine the effect of hypoxic preconditions on the ability of bone marrow stem cells culture mediated expression C-X-C chemokine receptor type 4 (CXCR4) and stromal cells derived factor-1 (SDF-1) in vitro.

Materials and Methods: Bone marrow mesenchymal stem cells (BMSCs) were derived from 12 femurs of 200 g Wistar male rats. The animals were euthanized before BMSCs isolation. BMSCs were divided into two groups, control group: Normoxic condition 21% O2 and treatment group: Hypoxic condition 1% O2. The characterization of BMSCs was analyzed using flow cytometry by cluster differentiation 34 and cluster differentiation 105. The expression of CXCR4 and SDF-1 measured using immunocytochemistry immunofluorescence label after 48-h incubation in a low-tension oxygen chamber with an internal atmosphere consisting of 95% N2, 5% CO2, and 1% O2. All data were subjected to a normality test and then analyzed using t-test statistic (p<0.05).

Results: The characterization of bone marrow stem cells showed positive cluster differentiation 34 and cluster differentiation 105. A hypoxic precondition (1% O2) in culture increases CXCR4 (p=0.000) and SDF-1 expression than normoxic conditions (p=0.000) (p<0.05).

Conclusion: Hypoxic preconditioning with 1% O2 increase CXCR4 and SDF1 expression.

Keywords: bone marrow stem cells, C-X-C chemokine receptor type 4, hypoxic preconditioning, mesenchymal stem cells, stromal cells derived factor-1.

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