Hypoxic preconditioning effect on stromal cells derived factor-1 and C-X-C chemokine receptor type 4 expression in Wistar rat's (Rattus norvegicus) bone marrow mesenchymal stem cells (in vitro study)

Vet World Vol.11 July-2018 Article-13

Research Article

Veterinary World, 11(7): 965-970

https://doi.org/10.14202/vetworld.2018.965-970

Sri Wigati Mardi Mulyani¹, Diah Savitri Ernawati², Eha Renwi Astuti¹, and Fedik Abdul Rantam^3,4

1. Department of Dentomaxillofacial Radiology, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia.
2. Department of Oral Medicine, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia.
3. Stem Cell Research Center and Development, Airlangga University Surabaya, Indonesia.
4. Lab of Virology and Immunology, Department of Microbiology, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia.

Background and Aim: To examine the effect of hypoxic preconditions on the ability of bone marrow stem cells culture mediated expression C-X-C chemokine receptor type 4 (CXCR4) and stromal cells derived factor-1 (SDF-1) in vitro.

Materials and Methods: Bone marrow mesenchymal stem cells (BMSCs) were derived from 12 femurs of 200 g Wistar male rats. The animals were euthanized before BMSCs isolation. BMSCs were divided into two groups, control group: Normoxic condition 21% O2 and treatment group: Hypoxic condition 1% O2. The characterization of BMSCs was analyzed using flow cytometry by cluster differentiation 34 and cluster differentiation 105. The expression of CXCR4 and SDF-1 measured using immunocytochemistry immunofluorescence label after 48-h incubation in a low-tension oxygen chamber with an internal atmosphere consisting of 95% N2, 5% CO2, and 1% O2. All data were subjected to a normality test and then analyzed using t-test statistic (p<0.05).

Results: The characterization of bone marrow stem cells showed positive cluster differentiation 34 and cluster differentiation 105. A hypoxic precondition (1% O2) in culture increases CXCR4 (p=0.000) and SDF-1 expression than normoxic conditions (p=0.000) (p<0.05).

Conclusion: Hypoxic preconditioning with 1% O2 increase CXCR4 and SDF1 expression. Keywords: bone marrow stem cells, C-X-C chemokine receptor type 4, hypoxic preconditioning, mesenchymal stem cells, stromal cells derived factor-1.

Keywords: bone marrow stem cells, C-X-C chemokine receptor type 4, hypoxic preconditioning, mesenchymal stem cells, stromal cells derived factor-1.

How to cite this article: Mulyani SWM, Ernawati DS, Astuti ER, Rantam FA (2018) Hypoxic preconditioning effect on stromal cells derived factor-1 and C-X-C chemokine receptor type 4 expression in Wistar rat's (Rattus norvegicus) bone marrow mesenchymal stem cells (in vitro study), Veterinary World, 11(7): 965-970.

Received: 25-01-2018 Accepted: 04-06-2018 Published online: 19-07-2018

Corresponding author: Diah Savitri Ernawati E-mail: diah-s-e@fkg.unair.ac.id

DOI: 10.14202/vetworld.2018.965-970

Copyright: Mulyani SWM, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.