Article history: Received: 22-03-2018, Accepted: 30-05-2018, Published online: 14-07-2018
Corresponding author: Wisnu Nurcahyo
E-mail: email@example.comCitation: Widiarso BP, Kurniasih K, Prastowo J, Nurcahyo W (2018) Morphology and morphometry of Haemonchus contortus exposed to Gigantochloa apus crude aqueous extract, Veterinary World, 11(7): 921-925.
Aim: Haemonchus contortus is the most pathogenic nematode infesting the digestive tract of goats and sheep worldwide leading to a tremendous loss in a variety of routes. Economic losses due to haemonchosis in subtropic and tropic areas are usually caused by poor weight gain, minimized growth, loss of production, and mortality. The prevalence of haemonchosis in Indonesia is 89.4% in goat, and annual loss achieved 1 million US dollars. This study evaluated in vitro effects of Gigantochloa apus crude aqueous extract as an anthelmintic on H. contortus morphology and morphometry.
Materials and Methods: Bligon goats which are naturally infected were collected from slaughtered goat from local slaughterhouses, namely Besi Sleman. Bligon goat's abomasum part was carefully examined and transported to the Parasitology Laboratory, University of Gadjah Mada, Yogyakarta. H. contortus was obtained from 4 to 6-month-old female goat from slaughterhouses in Yogyakarta area. H. contortus was collected from abomasum and put into a Petri dish containing 0.62% water saline. The number of H. contortus used for each concentration is 25. H. contortus was soaked in each concentration for 4 h. The figure of the parasites or parts of parasites was captured using camera Lucida, and they were measured using both objective micrometer and objective ocular micrometer. All the capturing processes were done with the help of Olympus Digital Camera under Olympus CX21 microscopic. Parasite morphology was identified in morphological and morphometric characters.
Results: Morphology of H. contortus revealed the cervical papillae bulge appears unclear shape and anterior end is more tapered. Vulvar flab control is not tapered, but vulvar flab which gets aware of G. apus crude aqueous extract looks more pointed. The gubernaculum appears irregular compared to gubernaculum control which tends to be more compact, and the posterior end form appears irregular more than posterior end control. Morphometry study of H. contortus indicates that it has a significant difference for body length, body width, cervical papillae, and spicule length in the male.
Conclusion: G. apus crude aqueous extract activity revealed morphology change such as cervical papillae, vulvar flab, gubernaculum, posterior end, and reduced morphometry measurement of H. contortus adult worms, notably in body length, body width, cervical papillae width, gubernaculum, and spicule length in males and body length, body width, cervical papillae width, and vulva length in females.
Keywords: bligon goat, crude aqueous extract, Haemonchus contortus, morphology, morphometry.
1. Kuchai, J.A., Ahmad, F., Chishti, M.Z., Tak, H., Ahmad, J.A.S. and Razool, M. (2012) A study on morphology and morphometry of Haemonchus contortus. Pak. J. Zool., 44(6): 1737-1741.
2. Mengist, Z., Abebe, N., Gugsa, G. and Kumar, N. (2014) Assesment of small ruminant haemonchosis and its associated risk factors in and around Finote selam, Ethiopia. IOSR JAVS, 7(12): 36-41. [Crossref]
3. Nasai, N.B., Abba, Y., Abdullah, F.F.J., Marimuthu, M., Tijjani, A., Sadiq, M.A., Mohammed, K., Chung, E.L.T. and Omar, M.A.B. (2016) In vitro larvicidal effects of ethanolic extract of Curcuma longa Linn. On Haemonchus larval stage. Vet. World, 9(4): 417-420. [Crossref] [PubMed] [PMC]
4. FAO. (1991) Food Losses Due to Non-infectious and Production Diseases in Developing Countries. FAO, United Nations.
5. Alemu, Z., Kechero,Y., Kabede, A. and dan Muhammed, A. (2014) Comparison of the in vitro inhibitory effect of doses of tannin rich plant extract and ivermectin on egg hatchability, larvae development, and adult Mortality of Haemonchus contortus. Acta Parasitol. Glob., 5(3): 160-168.
6. Alawa, C.B.I., Adamu, A.M., Gefu, J.O., Ajanusi, O.J., Abdu, P.A., Chiezy, N.P., Alawa, J.N. and dan Bowman, D.D. (2003) In vitro screening of two Nigerian medicinal plants (Vernona amygdalina and Annona senegalensis) for anthelmintic activity. J. Vet. Parasitol., 113: 73-81. [Crossref]
7. Akkari, H., Darghout, M.A. and Salem, H.B. (2008) Preliminary investigations of the anti-nematode activity of Acacia cyanophylla Lindl.: Excretion gastrointestinal nematode eggs in lambs browsing A. cyanophulla with and without PEG or grazing native grass. J. Small Rum. Res., 74(b): 78-83.
8. Suratiningsih, S., Rahayu, S.M. and Suhartati, F.M. (2013) Supplementation ethanol extract bamboo leaves Petung (Dendrocalamus asper) effect on concentration of N-NH3 and VFA total in in vitro. J. Sci. Ranch, 1(2): 590-596.
9. Daryatmo, J., Hartadi, H., Orskov, E.R., Adiwimarta, K., and Nurcahyo, W. (2010) screening of various forages for anthelmintic activity of Haemonchus contortus eggs. Adv. Anim. Biosci., 1: 113. [Crossref]
10. Reyaz, A. (2005) Epidemiology, Biology, and Pathogenicity of Nematodes in Ruminants of Kashmir. M.Phil. Dissertations. University of Kashmir. [PubMed]
11. Min, B.R., Barry, T.N., Attwood, G.T. and Menabb, W.C. (2003) The effect of condensed tannin on the nutrition of ruminants fed fresh temperates forages: A review. Anim. Feed Sci. Tech., 106: 3-19. [Crossref]
12. Zhong, R.Z., Sun, H.X., Liu, H.W. and Zhou, D.W. (2014) Effects of tannin acid on Haemonchus contortus larvae viability and immune responses of sheep white blood cells in vitro. Parasite Immunol., 36: 100-106. [Crossref] [PubMed]
13. Hoste, H., Jackson, F., Athanasiadou, S., Thamsborg, S.M. and Hoskin, S.O. (2006) The effect of tannin-rich plants on parasitic nematodes in ruminants. Trend Parasitol., 22(6): 253-261. [Crossref] [PubMed]
14. Molan, A.L., Waghorn, G.C. and McNabb, W.C. (2009) Condensed tannin and gastrointestinal parasites in sheep. Proc. N. Z. Soc. Anim. Prod., 69: 89-92.
15. Calvin, B.Z., Abiodoun, O.P., Georcelin, A.G., Laure, A.N., Purtrand, J.A., Blaise, A.G. and Sylvie, H.A. (2016) In vitro anthelmintic activity of aqueous extract of Crasscephalum crepidioides (benth) S. Moore on Haemonchus contortus. J. Exp. Integr. Med., 6: 1.
16. Sambodo, P., Prastowo, J., Kurniasih, K. and Indarjulianto, S. (2018) In vitro potential anthelmintic activity of Biophytum petersianum on Haemonchus contortus. Vet. World, 11(1): 1-4. [Crossref]17. Martinez-Ortiz-de-Montellano, C., Arroyo-Lopez, C., Fourquaux, I., Torres-Acosta, J.F.J., Sandoval-Castro, C.A. and Hoste, H. (2013) Scanning electron microscopy of Haemonchus contortus exposed to tannin-rich plants under in vivo and in vitro conditions. Exp. Parasitol., 133(3): 281-286. [Crossref] [PubMed]