Article history: Received: 10-03-2018, Accepted: 03-05-2018, Published online: 13-06-2018
Corresponding author: Mayank Rawat
E-mail: email@example.comCitation: Verma S, Rawat M, Kumawat S, Qureshi S, Mohd G, Tiwari AK (2018) Protective role of Brucella abortus specific murine antibodies in inhibiting systemic proliferation of virulent strain 544 in mice and guinea pig, Veterinary World, 11(6): 794-799.
Aim: The major objective of the investigation was to evaluate the hitherto uncharacterized potential of Brucella-specific antibodies to win the battle against virulent Brucella abortus infection.
Materials and Methods: Brucella-specific immune serum was raised in mice. The antibody titer of serum was determined by standard tube agglutination test and indirect enzyme-linked immunosorbent assays (iELISA). Groups of mice and guinea pigs were passively immunized with serum containing specific agglutinin titers. 24 h after immunization, all animals along with unimmunized controls were challenged with B. abortus S544. Total B. abortus S544 counts in the spleen of each animal collected on the 7th day of challenge was determined to evaluate the protective index (PI) of anti-Brucella serum by statistical analysis.
Results: A dose-dependent protective response to immune mice serum was observed in both experimental models though the values of PI of mice were higher than those obtained for guinea pigs. The PI values in mice passively immunized with 50 IU or 25 IU antibodies were 1.38 and 0.69, respectively. In guinea pigs, however, animals passively immunized with 50 IU or 25 IU antibodies showed PI values equivalent to 0.79 and 0.41, respectively.
Conclusion: The observations support our hypothesis that the presence of antibodies inhibits the initial multiplication and eventual colonization of systemic organs by B. abortus. Therefore, a predominant antibody-mediated response induced by a vaccine is expected to protect the animal against the most severe clinical outcome of infection.
Keywords: brucellosis, humoral immunity, mice, passive protection, protective index.
1. Manish, K.P., Rajesh, C., Teena, R. and Sunil, K. (2013) Brucellosis-an updated review of the disease. Ind. J. Anim. Sci., 83: 3-16.
2. World Health Organization. (2014) The Control of Neglected Zoonotic Diseases, Report of the 4th International Meeting Held at WHO Headquarters, Geneva, Switzerland. p19-20.
5. Kose, S., Serin, S.S., Akkoclu, G., Kuzucu, L., Ulu, Y., Ersan, G. and Oguz, F. (2014) Clinical manifestations, complications, and treatment of brucellosis: Evaluation of 72 cases. Turk. J. Med. Sci., 44: 220-223. [Crossref] [PubMed]
6. Roushan, M.R.H. and Ebrahimpour, S. (2015) Human brucellosis: An overview. Caspian J. Intern. Med., 6(1): 46-47.
9. Jain, L., Rawat, M., Prajapati, A., Tiwari, A.K., Kumar, B., Chaturvedi, V.K., Saxena, H.M., Ramakrishnan, S., Kumar, J. and Kerketta, P. (2015) Protective immune-response of aluminium hydroxide gel adjuvanted phage lysate of Brucella abortus S19 in mice against direct virulent challenge with B. abortus 544. Biologicals, 43: 369-376. [Crossref] [PubMed]
10. Xavier, M.N., Winter, M.G., Spees, A.M., Nguyen, K., Atluri, V.L., Silva, T.M., Baumler, A.J., Muller, W., Santos, R.L. and Tsolis, R.M. (2013) CD4+ T cell-derived IL-10 promotes Brucella abortus persistence via modulation of macrophage function. PLoS Pathog., 9: e1003454. [Crossref]
11. Gomez, G., Adams, L.G., Ficht, A.R. and Ficht, T.A. (2013) Host-Brucella interactions and the Brucella genome as tools for subunit antigen discovery and immunization against brucellosis. Front. Cell Infect. Microbiol., 3: 17. [Crossref] [PubMed] [PMC]
13. Casadevall, A. and Pirofski, L.A. (2006) A reappraisal of humoral immunity based on mechanisms of antibody-mediated protection against intracellular pathogens. Adv. Immunol., 91: 1-44. [Crossref]
15. Araya, L.N., Elzer, P.H., Rowe, G.E., Enright, F.M. and Winter, A.J. (1989) Temporal development of protective cell-mediated and humoral immunity in BALB/c mice infected with Brucella abortus. J. Immunol., 143: 3330-3337. [PubMed]
17. Indian Pharmacopoeia Commission. (2014) Government of India, Ministry of Health and Family Welfare. Indian Pharmacopoeia Commission, Ghaziabad, India. 3: 2.2.11.
18. OIE. (2008) Bovine brucellosis. In: OIE Manual of Diagnostic Tests and Vaccines for Terrestrial Animals. 6th ed. OIE, Paris, France. p1-35.
19. Alton, G.G., Jones, L.M. and Pietz, D.E. (1975) Laboratory techniques in brucellosis. Monogr. Ser. World Health Organ., 55: 1-163.
20. Westphal, O. and Jann, K. (1965) Bacterial lipopolysaccharides extraction with phenol-water and further applications of the procedure. Meth. Carbohydr. Chem., 5: 83-91.
24. Canning, P.C., Deyoe, B.L. and Roth, J.A. (1988) Opsonin-dependent stimulation of bovine neutrophil oxidative metabolism by Brucella abortus. Am. J. Vet. Res., 49: 160. [PubMed]
25. Winter, A.J., Duncan, J.R., Santisteban, C.G., Douglas, J.T. and Adams, L.G. (1989) Capacity of passively administered antibody to prevent establishment of Brucella abortus infection in mice. Infect. Immun., 57: 3438-3444. [PubMed] [PMC]
27. Ko, J. and Splitter, G.A. (2003) Molecular host-pathogen interaction in brucellosis: Current understanding and future approaches to vaccine development for mice and humans. Clin. Microbiol. Rev., 16: 65-78. [Crossref]
29. Adone, R., Francia, M., Pistoia, C., Petrucci, P., Pesciaroli, M. and Pasquali, P. (2012) Protective role of antibodies induced by Brucella melitensis B115 against B. melitensis and Brucella abortus infections in mice. Vaccine, 30(27): 3992-3995. [Crossref] [PubMed]
30. Vitry, M.A., Mambres, D.H., Trez, C.D., Akira, S., Ryffel, B., Letesson, J.J. and Muraille, E. (2014) Humoral immunity and CD4+ Th1 cells are both necessary for a fully protective immune response upon secondary infection with Brucella melitensis. J. Immunol., 192(8): 3740-3752. [Crossref] [PubMed]
31. Ducrotoya, M., Bertub, W.J., Matopec, G., Cadmusd, S., Conde-Alvareze, R., Gusib, A.M., Welburna, S., Ocholib, R., Blascof, J.M. and Moriyone, I. (2017) Brucellosis in Sub-Saharan Africa: Current challenges for management, diagnosis and control. Acta Trop., 165: 179-193. [Crossref]32. Dorneles, E.M.S., Lima, G.K., Teixeira-Carvalho, A., Araujo, M.S.S., Martins-Filho, O.A., Sriranganathan, N., Qublan, H.A., Heinemann, M.B. and Lage, A.P. (2015) Immune response of calves vaccinated with Brucella abortus S19 or RB51 and revaccinated with RB51. PLoS One, 10(9): e0136696. [Crossref]