Article history: Received: 18-02-2018, Accepted: 07-05-2018, Published online: 09-06-2018
Corresponding author: Khaleel I. Jawasreh
E-mail: email@example.comCitation: Jawasreh KI, Ababneh MM, Ismail ZB, Younes AMB, Al Sukhni I (2018) Genetic diversity and population structure of local and exotic sheep breeds in Jordan using microsatellites markers, Veterinary World, 11(6): 778-781.
Aim: This study was conducted to study the genetic and population structure of local (Awassi) and exotic (Romanov, Charollais, Assaf, and Suffolk) sheep breeds in Jordan using eight microsatellite markers.
Materials and Methods: A total of 125 sheep were used (25 from each breed) in the study. The number of alleles (A), the mean values of observed (Ho) and expected (He) heterozygosity, polymorphism information content (PIC), fixation index as a measure of heterozygote deficiency or excess, and Hardy-Weinberg equilibrium (HWE) were analyzed using PopGen and CERVUS softwares. Nei's standard genetic distances among breeds and dendrogram of unweighted pair group method with arithmetic mean (UPGMA) were calculated and constructed using PopGen software.
Results: A total of 40 alleles were detected with an average number of alleles of 5. The mean Ho value was higher than the mean He value for all breeds. Awassi breed showed the highest average PIC value while Romanov had the lowest. There was a significant (p<0.05) deviation from HWE at each locus within and between breeds. Deviations from HWE were found to be highly significant for all markers except OARFCP304 locus. The genetic distance estimates revealed a close relationship between Romanov and Charollais and between Awassi and Charollais. In the UPGMA dendrogram, Charollais, Romanov, and Awassi breeds were placed together in one main cluster while Assaf was in a different subcluster. Awassi was placed alone in a second main cluster.
Conclusion: Results of this study offer insight toward the genetic conservation of the studied breeds and a base on which breeding plans can be made.
Keywords: genetic diversity, Jordan, microsatellites, sheep breeds.
1. Abu Zanat, M.M., Mekdadi, H.A. and Tabbaa, M.J. (2005) Production systems of small ruminants in middle Badia of Jordan. Dirasat, 32: 205-213.
2. Jaber, L.S., Habre, A., Rawda, N., Abisaid, M., Barbour, E.K. and Hamadeh, S.K. (2004) The effect of water restriction on certain physiological parameters in Awassi sheep. Small Rum. Res., 54: 115-120. [Crossref]
3. Diez-Tascon, C., Littlejohn, R.P., Almeida, P.A. and Crawford, A.M. (2000) Genetic variation within the Merino sheep breed: Analysis of closely related populations using microsatellites. Anim. Genet., 31: 243-251. [Crossref]
5. Arora, R. and Bhatia, S. (2004) Genetic structure of Muzzafarnagri sheep based on microsatellite analysis. Small Rum. Res., 54: 227-230. [Crossref]
6. Bozzi, R., Degl'Innocenti, P., Rivera Diaz, P., Nardia, L., Crovettia, A., Sargentinia, C. and Giorgettia, A. (2009) Genetic characterization and breed assignment in five Italian sheep breeds using microsatellite markers. Small Rum. Res., 85: 50-57. [Crossref]
7. Khan, M.A., Husain, S.S., Alam, M.R., Teneva, A., Han, J.L. and Faruque, M.O. (2009) Genetic relationship in different sheep populations of Bangladesh based on microsatellite markers. J. Bangladesh Agril. Univ., 7: 291-294.
8. Tariq, M.M., Bajwa, M.A., Jawasreh, K., Awan, M.A., Abbas, F., Waheed, A., Rafeeq, M., Wadood, A., Khan, K.U., Rashid, N., Atique, M.A. and Bukhari, F.A. (2012) Characterization of four indigenous sheep breeds of Balochistan, Pakistan by random amplified polymorphic DNAs. Afr. J. Biotechnol., 11: 2581-2586. [Crossref]
9. Ghazy, A., Mokhtar, S., Eid, M., Amin, A., Elzarei, M., Kizaki, K. and Hashizume, K. (2013) Genetic diversity and distances of three Egyptian local sheep breeds using microsatellite markers. Res. Zool., 3: 1-9.
10. Jawasreh, K., Al-Rawashdeh, I.M., Al-Majali, A., Talafha, H., Eljarah, A. and Awawdeh, F. (2011) Genetic relatedness among Jordanian local Awassi lines Baladi, Sagri, and Blackface and the black Najdi breed using RAPD analysis. Genom. Quant. Genet., 2: 31-36.
11. Yeh, F.C., Boyle, T., Rongcai, Y., Ye, Z. and Xian, J.M. (1999) Popgene, Version 1.31. A Microsoft Windows Based Freeware for Population Genetic Analysis. University of Alberta, Edmonton.
12. Marshall, T.C., Slate, J., Kruuk, L.E. and Pemberton, J.M. (1998) Statistical confidence for likelihood-based paternity inference in natural population. Mol. Ecol., 7: 639-655. [Crossref] [PubMed]
13. Nei, M. (1978) Estimation of average heterozygosity and genetic distance from a small number of individuals. Genet, 89: 583-590.
14. Radha, P., Sivaselvam, S.N., Kumarasamy, P. and Kumanan, K. (2011) Genetic diversity and bottleneck analysis of Kilakarsal sheep by microsatellite markers. Indian J. Biotechnol., 10: 52-55.
15. Al-Atiyat, R.M., Tabbaa, M.J., Salameh, N.M., Tarawneh, K.A., Al-Shmayla, L. and Al-Tamimie, H.J. (2012) Analysis of genetic variation of fat tailed-sheep in southern region of Jordan. Asian J. Anim. Vet. Adv., 7: 376-389. [Crossref]
16. Sodhi, M., Mukesh, M. and Bhatia, S. (2006) Characterizing Nali and Chokla sheep differentiation with microsatellite markers. Small Rum. Res., 65: 185-192. [Crossref]
17. Dashab, G.R., Aslaminejad, A., Nassiri, M., Esmailizadeh, A.K. and Saghi, D.A. (2011) Analysis of genetic diversity and structure of Buluchi sheep by microsatellite markers. Trop. Subtrop. Agroecosys., 14: 1047-1054.
18. Alvarez, I., Royo, L.J., Fernandez, I., Gutierrez, J.P., Gomez, E. and Goyache, F. (2004) Genetic relationships and admixture among sheep breeds from Northern Spain assessed using microsatellites. J. Anim. Sci., 82: 2246-2252. [Crossref] [PubMed]
19. Uzun, M., Gutierrez-Gil, B., Arranz, J.J., Primitivo, F.S., Saatci, M., Kaya, M. and Bayon, Y. (2006) Genetic relationships among Turkish sheep. Genet. Sel. Evol., 38: 513-524. [Crossref] [PubMed] [PMC]20. Kawecka, A. and Katarzyna, P. (2011) Characteristics of the genetic structure of native sheep breeds. Ann. Anim. Sci., 11: 371-382.