doi: 10.14202/vetworld.2018.316-320
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Article history: Received: 30-10-2017, Accepted: 10-02-2018, Published online: 16-03-2018
Corresponding author: Neeraj Shrivastava
E-mail: neerajvet32@gmail.com
Citation: Shrivastava N, Sharma V, Shrivastav A, Nayak A, Rai AK (2018) Prevalence and characterization of Panton-Valentine leukocidin-positive Staphylococcus aureus in bovine milk in Jabalpur district of Madhya Pradesh, India, Veterinary World, 11(3): 316-320.Aim: The study aimed to investigate the Panton-Valentine leukocidin (PVL)-positive Staphylococcus aureus in bovine milk due to its public health significance.
Materials and Methods: A total of 400 milk samples of bovines taken from different dairy farms and outlets of Jabalpur were screened for the S. aureus and methicillin-resistant S. aureus (MRSA). The strains were tested for the PVL gene and antimicrobial sensitivity toward 10 different classes of antimicrobial agents. The PVL-positive S. aureus strains were further characterized by staphylococcal protein A or spa typing.
Results: The prevalence of PVL-positive S. aureus was 10.53%. All the isolates positive for the PVL were resistant to methicillin, while the methicillin-sensitive S. aureus isolates were negative for the PVL. Five different spa types were found.
Conclusion: The presence of PVL-positive MRSA in bovine milk close to consumer poses a potential public health risk to the community.
Keywords: bovine milk, methicillin-resistant Staphylococcus aureus, Panton-Valentine leukocidin.
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