Article history: Received: 20-01-2018, Accepted: 06-04-2018, Published online: 02-05-2018
Corresponding author: Ajay Kumar Dang
E-mail: email@example.comCitation: Alhussien MN, Dang AK (2018) Milk somatic cells, factors influencing their release, future prospects, and practical utility in dairy animals: An overview, Veterinary World, 11(5): 562-577.
Milk somatic cells (SCs) are a mixture of milk-producing cells and immune cells. These cells are secreted in milk during the normal course of milking and are used as an index for estimating mammary health and milk quality of dairy animals worldwide. Milk SC is influenced by cow productivity, health, parity, lactation stage, and breed of an animal. Any change in environmental conditions, poor management practices, and also stressful conditions significantly increases the amount of SC coming in milk. Better hygiene and proper nutrition help in reducing milk SC. Milk with low SC means better milk products with a longer shelf life. The present review describes the role of SCs (both secretory and immune) in milk, their role in maintaining the integrity of the mammary gland, and factors affecting their release in milk. This information may help to reduce milk somatic cell counts (SCCs) and to establish differential SCC standards.
Keywords: dairy animals, factors influencing, milk, somatic cells release, utility.
1. Petzer, I.M., Karzis, J., Donkin, E.F., Webb, E.C. and Etter, E. (2017) Somatic cell count thresholds in composite and quarter milk samples as indicator of bovine intramammary infection status. Onderstepoort J. Vet. Res., 84: a1269. [Crossref]
2. Sharma, N., Singh, N.K. and Bhadwal, M.S. (2011) Relationship of somatic cell count and mastitis: An overview. Asian Aust. J. Anim. Sci., 24: 429-438. [Crossref]
5. Hillerton, J.E. (1999) Redefining mastitis based on somatic cell count. IDF. Bull., 345: 4-6.
6. Vishnoi, P.C., Dang, A.K. and Kapila, S. (2007) In vitro phagocytic activity of neutrophils in blood, normal and infected milk of Murrah buffaloes. Buffalo J., 1: 51-59.
7. Dang, A.K., Kapila, S., Singh, C. and Sehgal, J.P. (2008) Milk differential cell counts and compositional changes in cows during different physiological stages. Milchwissenschaft, 63: 239-242.
8. Ezzat Alnakip, M., Quintela-Baluja, M., Bohme, K., Fernandez-No, I., Caama-o-Antelo, S., Calo-Mata, P. and Barros-Velazquez, J. (2014) The immunology of mammary gland of dairy ruminants between healthy and inflammatory conditions. J. Vet. Med., 2014: 1-31. [Crossref] [PubMed] [PMC]
9. Swain, D.K., Kushwah, M.S., Kaur, M., Patbandha, T.K., Mohanty, A.K. and Dang, A.K. (2014) Formation of NET, phagocytic activity, surface architecture, apoptosis and expression of toll-like receptors 2 and 4 (TLR2 and TLR4) in neutrophils of mastitic cows. Vet. Res. Commun., 38: 209-219. [Crossref]
10. Swain, D.K., Kushwah, M.S., Kaur, M. and Dang, A.K. (2015) Neutrophil dynamics in the blood and milk of crossbred cows naturally infected with Staphylococcus aureus. Vet. World, 8: 336-345. [Crossref] [PubMed] [PMC]
11. Leitner, G., Chaffer, M., Krifucks, O., Glickman, A., Ezra, E. and Saran, A. (2000) Milk leucocyte populations in heifers free of udder infection. Zoonoses Public Health, 47: 133-138. [Crossref]
13. Alhussien, M., Kaur, M., Manjari, P., Kimothi, S.P., Mohanty, A.K. and Dang, A.K. (2015) A comparative study on the blood and milk cell counts of healthy, subclinical, and clinical mastitis Karan fries cows. Vet. World, 8: 685-689. [Crossref] [PubMed] [PMC]
14. Alhussien, M., Manjari, P., Sheikh, A.A., Seman, S.M., Reddi, S., Mohanty, A.K. and Dang, A.K. (2016) Immunological attributes of blood and milk neutrophils isolated from crossbred cows during different physiological conditions. Czech J. Anim. Sci., 61: 223-231. [Crossref]
15. Dang, A.K., Kapila, S., Tomar, P. and Singh, C. (2007) Relationship of blood and milk cell counts with mastitic pathogens in Murrah buffaloes. Ital. J. Anim. Sci., 6: 821-824. [Crossref]
16. Dang, A.K., Kapila, S., Tomar, P. and Singh, C. (2007) Immunity of the buffalo mammary gland during different physiological stages. Asian Aust. J. Anim. Sci., 20: 1174-1181. [Crossref]
17. Morgante, M., Ranucci, S., Pauselli, M., Casoli, C. and Duranti, E. (1996) Total and differential cell count in milk of primiparous comisana ewes without clinical signs of mastitis. Small Rumin. Res., 21: 265-271. [Crossref]
18. Boulaaba, A., Grabowski, N. and Klein, G. (2011) Differential cell count of caprine milk by flow cytometry and microscopy. Small Rumin. Res., 97: 117-123. [Crossref]
19. Hamed, H., Gargouri, A., Hachana, Y. and El Feki, A. (2010) Comparison between somatic cell and leukocyte variations throughout lactation in camel (Camelus dromedarius) and cow's milk. Small Rumin. Res., 94: 53-57. [Crossref]
20. Dosogne, H., Vangroenweghe, F., Mehrzad, J., Massart-Leen, A.M. and Burvenich, C. (2003) Differential leukocyte count method for bovine low somatic cell count milk. J. Dairy Sci., 86: 828-834. [Crossref]
22. Alhussien, M., Manjari, P., Mohammed, S., Sheikh, A.A., Reddi, S., Dixit, S. and Dang, A.K. (2016) Incidence of mastitis and activity of milk neutrophils in Tharparkar cows reared under semi-arid conditions. Trop. Anim. Health Prod., 48: 1291-1295. [Crossref] [PubMed]
23. Vishnoi, P.C. and Dang, A.K. (2007) Changes in blood and milk DLC and its effect on milk composition in Murrah buffaloes (Bubalus bubalis) suffering from clinical mastitis. Indian J. Dairy Sci., 60: 286-292.
24. De, K., Mukherjee, J., Dang, A.K., Singh, C. and Prasad, S. (2010) Studies on differential leukocyte counts in the colostrum of Murrah buffalo. Rev. Vet., 21: 600-602.
26. Gargouri, A., Hamed, H. and Elfeki, A. (2008) Total and differential bulk cow milk somatic cell counts and their relation with lipolysis. Livestock Sci., 113: 274-279. [Crossref]
27. Alhussien, M.N. and Dang, A.K. (2017) Integrated effect of seasons and lactation stages on the plasma inflammatory cytokines, function and receptor expression of milk neutrophils in Sahiwal (Bos indicus) cows. Vet. Immunol. Immunopathol., 191: 14-21. [Crossref]
28. Damm, M., Holm, C., Blaabjerg, M., Bro, M.N. and Schwarz, D. (2017) Differential somatic cell count-a novel method for routine mastitis screening in the frame of dairy herd improvement testing programs. J. Dairy Sci., 100(6): 4926-4940. [Crossref] [PubMed]
29. Mukherjee, J. and Dang, A.K. (2011) Immune activity of milk leukocytes during early lactation period in high and low yielding crossbred cows. Milchwissenschaft, 66: 384-388.
30. Cinar, M., Serbester, U., Ceyhan, A. and Gorgulu, M. (2015) Effect of somatic cell count on milk yield and composition of first and second lactation dairy cows. Ital. J. Anim. Sci., 14: 105-108. [Crossref]
31. Sharma, T., Das, P.K., Ghosh, P.R., Banerjee, D. and Mukherjee, J. (2017) Association between udder morphology and in vitro activity of milk leukocytes in high yielding crossbred cows. Vet. World, 10: 342-347. [Crossref] [PubMed] [PMC]
32. Kennedy, B. W., Sethar, M. S., Tong, A. K. W., Moxley, J. E. and Downey, B. R. (1982) Environmental factors influencing test-day somatic cell counts in Holsteins. J. Dairy Sci., 65: 275-280. [Crossref]
33. Sheldrake, R.F., Hoare, R.J.T. and McGregor, G.D. (1983) Lactation stage, parity, and infection affecting somatic cells, electrical conductivity, and serum albumin in milk. J. Dairy Sci., 66: 542-547. [Crossref]
34. Singh, M. and Ludri, R.S. (2000) Somatic cell counts in Murrah buffaloes (Bubalus bubalis) during different stages of lactation, parity and season. Asian Aust. J. Anim. Sci., 14: 189-192. [Crossref]
35. Singh, M. and Dang, A.K. (2002) Somatic cell count of milk. Technical Book. Published by National Dairy Research Institute, Karnal, India. p1-25.
36. Goncalves, J.L., Cue, R.I., Botaro, B.G., Horst, J.A., Valloto, A.A. and Santos, M.V. (2018) Milk losses associated with somatic cell counts by parity and stage of lactation. J. Dairy Sci., 80: 3219. [Crossref]
37. De Vliegher, S., Barkema, H.W., Stryhn, H., Opsomer, G. and de Kruif, A. (2005) Impact of early lactation somatic cell count in heifers on milk yield over the first lactation. J. Dairy Sci., 88: 938-947. [Crossref]
38. Moroni, P., Rossi, C.S., Pisoni, G., Bronzo, V., Castiglioni, B. and Boettcher, P.J. (2006) Relationships between somatic cell count and intramammary infection in buffaloes. J. Dairy Sci., 89: 998-1003. [Crossref]
39. Mukherjee, J., Varshney, N., Chaudhury, M., Mohanty, A.K. and Dang, A.K. (2013) Immune response of the mammary gland during different stages of lactation cycle in high versus low yielding Karan Fries crossbred cows. Livestock Sci., 154: 215-223. [Crossref]
40. Svensson, C., Nyman, A.K., Waller, K.P. and Emanuelson, U. (2006) Effects of housing, management, and health of dairy heifers on first-lactation udder health in southwest Sweden. J. Dairy Sci., 89: 1990-1999. [Crossref]
41. Saravanan, R., Das, D.N., De, S. and Panneerselvam, S. (2015) Effect of season and parity on somatic cell count across zebu and crossbred cattle population. Indian J. Anim. Res., 49: 383-387. [Crossref]
42. Geneurova, V., Hanns, O., Gabriel, B. and Zvackova, I. (1993) Somatic cell counts of milk in relation to production factors. Zivocisna Vyroba, 38: 359-367.
43. Laevens, H., Deluyker, H., Schukken, Y.H., De Meulemeester, L., Vandermeersch, R., De Muelenaere, E. and De Kruif, A. (1997) Influence of parity and stage of lactation on the somatic cell count in bacteriologically negative dairy cows. J. Dairy Sci., 80: 3219-3226. [Crossref]
44. Schepers, A.J., Lam, T.J.G.M., Schukken, Y.H., Wilmink, J.B.M. and Hanekamp, W.J.A. (1997) Estimation of variance components for somatic cell counts to determine thresholds for uninfected quarters. J. Dairy Sci., 80: 1833-1840. [Crossref]
45. Dang, A.K., Mukherjee, J., Chaudhury, M., Shiv, P., Mohanty, A.K., Kapila, S. and Kapila, R. (2014) In vitro phagocytic activity of blood and milk neutrophils against Saccharomyces cerevisiae in primiparous and multiparous Karan Fries crossbred cows throughout the dry period and lactation cycle. Indian J. Anim. Sci., 84: 262-266.
46. Alhussien, M.N. and Dang, A.K. (2017) Diurnal rhythm in the counts and types of milk somatic cells, neutrophil phagocytosis and plasma cortisol levels in Karan Fries cows during different seasons and parity. Biol. Rhythm. Res., 49: 187-199. [Crossref]
47. Berry, D.P., Lee, J.M., Macdonald, K.A., Stafford, K., Matthews, L. and Roche, J.R. (2007) Associations among body condition score, body weight, somatic cell count, and clinical mastitis in seasonally calving dairy cattle. J. Dairy Sci., 90: 637-648. [Crossref]
48. Morse, D., De Lorenzo, M.A., Wilcox, C.J., Collier, R.J., Natzke, R.P. and Bray, D.R. (1988) Climatic effects on occurrence of clinical mastitis. J. Dairy Sci., 71: 848-853. [Crossref]
49. Clements, A.C.A., Pfeiffer, D.U. and Hayes, D. (2005) Bayesian spatio-temporal modelling of national milk-recording data of seasonal-calving New Zealand dairy herds. Prev. Vet. Med., 71: 183-196. [Crossref] [PubMed]
50. Mukherjee, J., De, K., Chaudhury, M. and Dang, A.K. (2015) Seasonal variation in in vitro immune activity of milk leukocytes in elite and non-elite crossbred cows of Indian sub-tropical semi-arid climate. Biol. Rhythm. Res., 46: 425-433. [Crossref]
51. Bernabucci, U., Basirico, L., Morera, P., Dipasquale, D., Vitali, A., Cappelli, F.P. and Calamari, L.U.I.G.I. (2015) Effect of summer season on milk protein fractions in Holstein cows. J. Dairy Sci., 98: 1815-1827. [Crossref] [PubMed]
52. Bombade, K., Kamboj, A., Alhussien, M.N., Mohanty, A.K. and Dang, A.K. (2017) Diurnal variation of milk somatic and differential leukocyte counts of Murrah buffaloes as influenced by different milk fractions, seasons and parities. Biol. Rhythm. Res., 49: 151-163. [Crossref]
53. Dang, A.K. and Anand, S.K. (2007) Effect of milking systems on the milk somatic cell counts and composition. Livestock Res. Rural Dev., 19: 1-9.
54. Castro, A., Pereira, J.M., Amiama, C. and Bueno, J. (2015) Typologies of dairy farms with automatic milking system in northwest Spain and farmers' satisfaction. Ital. J. Anim. Sci., 14: 3559. [Crossref]
55. Castro, A., Pereira, J.M., Amiama, C. and Barrasa, M. (2017) Long-term variability of bulk milk somatic cell and bacterial counts associated with dairy farms moving from conventional to automatic milking systems. Ital. J. Anim. Sci., 17: 218-225. [Crossref]
56. Paape, M.J., Wiggans, G.R., Bannerman, D.D., Thomas, D.L., Sanders, A.H., Contreras, A. and Miller, R.H. (2007) Monitoring goat and sheep milk somatic cell counts. Small Rumin. Res., 68: 114-125. [Crossref]
57. Koc, A. and Kizilkaya, K.A.D.I.R. (2009) Some factors influencing milk somatic cell count of Holstein Friesian and Brown Swiss cows under the Mediterranean climatic conditions. Arch. Tierz., 52: 124-133.
58. Ivkic, Z., Spehar, M., Bulic, V., Mijic, P., Ivankovic, A. and Solic, D. (2012) Estimation of genetic parameters and environmental effects on somatic cell count in Simmental and Holstein breeds. Mljekarstvo, 62: 143-150.
59. Ahlawat, K., Dang, A.K. and Singh, C. (2008) Relationships of teat and udder shape with milk SCC in primiparous and multiparous Sahiwal cows. Indian J. Dairy Sci., 61: 152-156.
60. Sharma, T., Kumar, D.P., Ghosh, P.R., Banerjee, D., Chandra, D.B. and Mukherjee, J. (2016) Alteration in the in vitro activity of milk leukocytes during different parity in high yielding cross-bred cows. Biol. Rhythm. Res., 47: 519-527. [Crossref]
61. Junior, L., Ferreira, J.E., Lange, C.C., Brito, M.A.V.P., Santos, F.R., Silva, M.A.S. and Souza, G.N.D. (2012) Relationship between total bacteria counts and somatic cell counts from mammary quarters infected by mastitis pathogens. Cienci. Rural, 42: 691-696. [Crossref]
62. Souza, G.N., Brito, J.R.F., Moreira, E.C., Brito, M.A.V.P. and Silva, M.V.G.B. (2009) Somatic cell counts variation in dairy cows according to mastitis pathogens. Arq. Bras. Med. Vet. Zootec., 61: 1015-1020. [Crossref]
63. Bi, Y., Wang, Y.J., Qin, Y., Vallverdu, R.G., Garcia, J.M., Sun, W. and Cao, Z. (2016) Prevalence of bovine mastitis pathogens in bulk tank milk in China. PLoS One, 11: e0155621. [Crossref]
64. Mukherjee, J., Chaudhury, M. and Dang, A.K. (2017) Alterations in the milk yield and composition during different stages of lactation cycle in elite and non-elite Karan-Fries cross-bred cows (Holstein Friesian x Tharparkar). Biol. Rhythm. Res., 48: 499-506. [Crossref]
65. Mukherjee, J., Chaudhury, M. and Dang, A.K. (2017) Alterations in the relative abundance of haptoglobin (Hp) transcripts in total milk somatic cells during different stages of lactation cycle in high yielding cross-bred cows. Biol. Rhythm. Res., 48: 577-581. [Crossref]
66. Le Marechal, C., Thiery, R., Vautor, E. and Le Loir, Y. (2011) Mastitis impact on technological properties of milk and quality of milk products-a review. Dairy Sci. Technol., 91: 247-282. [Crossref]
67. Shuster, D.E., Harmon, R.J., Jackson, J.A. and Hemken, R.W. (1991) Suppression of milk production during endotoxin-induced mastitis. J. Dairy Sci., 74: 3763-3774. [Crossref]
68. Petrovski, K. (2006) Milk Composition Changes During Mastitis. Dairy Vets Newsletter., 23: 7-12.
69. dos Reis, C.B.M., Barreiro, J.R., Mestieri, L., de Felicio Porcionato, M.A. and dos Santos, M.V. (2013) Effect of somatic cell count and mastitis pathogens on milk composition in Gyr cows. BMC Vet. Res., 9: 67. [Crossref] [PubMed] [PMC]
70. Ramos, T.M., Costa, F.F., Pinto, I.S.B., Pinto, S.M. and Abreu, L.R. (2015) Effect of somatic cell count on bovine milk protein fractions. J. Anal. Bioanal. Tech., 6:269.
71. Savic, N.R., Mikulec, D.P. and Radovanovic, R.S. (2017) Somatic Cell Counts in Bulk Milk and their Importance for Milk Processing. In: IOP Conference Series: Earth and Environmental Science. Vol. 85. IOP Publishing. p012085. [Crossref]
72. Sert, D., Mercan, E., Aydemir, S. and Civelek, M. (2016) Effects of milk somatic cell counts on some physicochemical and functional characteristics of skim and whole milk powders. J. Dairy Sci., 99: 5254-5264. [Crossref] [PubMed]
73. Bobbo, T., Cipolat-Gotet, C., Bittante, G. and Cecchinato, A. (2016) The nonlinear effect of somatic cell count on milk composition, coagulation properties, curd firmness modeling, cheese yield, and curd nutrient recovery. J. Dairy Sci., 99: 5104-5119. [Crossref]
74. Charismiadou, M., Karla, G., Theodorou, G., Goliomytis, M. and Politis, I. (2015) The effect of health status of the udder on plasminogen activator activity of milk somatic cells in ovine milk. Small Rumin. Res., 133: 54-57. [Crossref]
75. Sanchez-Macias, D., Morales-delaNuez, A., Torres, A., Hernandez-Castellano, L.E., Jimenez-Flores, R., Castro, N. and Arguello, A. (2013) Effects of addition of somatic cells to caprine milk on cheese quality. Int. Dairy J., 29: 61-67. [Crossref]
76. Paape, M.J. and Tucker, H.A. (1966) Somatic cell content variation in fraction-collected milk. J. Dairy Sci., 49: 265-267. [Crossref]
77. Miller, R.H., Paape, M.J., Peters, R.R. and Young, M.D. (1990) Total and differential somatic cell counts and N-Acetyl-β-D-glucosaminidase activity in mammary secretions during dry period. J. Dairy Sci., 73: 1751-1755. [Crossref]
78. Olejnik, P. (1994) Variation of somatic cell counts in colostrum and milk of first and second lactation cows during ten days after calving. Vet. Med., 39: 519-532.
80. Santoshi, P., Oberoi, P.S., Alhussien, M.N. and Dang, A.K. (2018) Combined effect of trisodium citrate and vitamin E supplementation during the transition period on body weight and other production parameters in Sahiwal cows. Indian J. Dairy Sci., 71: 78-83.
81. Pizauro, L.J.L., Silva, D.G., Santana, A.M., Clemente, V., Lara, GH.B., Listoni, F.J.P. and Fagliari, J.J. (2014) Prevalence and etiology of buffalo mastitis and milk somatic cell count in dry and rainy seasons in a buffalo herd from Analandia, Sao Paulo State, Brazil. Arq. Bras. Med. Vet. Zootec., 66: 1703-1710. [Crossref]
82. Sales, D.C., Rangel, A.H.N., Galvao, J.G.B. Jr., Borba, L.H.F., Freitas, A.R. and Moura, E.O. (2016) Relationship between the yield of mozzarella cheese of buffalo's milk, milk quality and the recovery of constituents in whey. J. Anim. Sci., 94: 246. [Crossref]
83. Dang, A.K. and Ludri, R.S. (2001) A study on the scanning electron microscopy of the buffalo mammary gland. Asian Aust. J. Anim. Sci., 14: 101-103. [Crossref]
84. De, K., Mukherjee, J., Prasad, S. and Dang, A.K. (2011) Effect of different physiological stages and managemental practices on milk somatic cell counts of Murrah Buffaloes. Buff. Bull., 30:72-74, 99.
85. Dang, A.K., Prasad, S., De, K., Pal, S., Mukherjee, J., Sandeep, I.V.R. and Kapila, R. (2013) Effect of supplementation of vitamin E, copper and zinc on the in vitro phagocytic activity and lymphocyte proliferation index of peripartum Sahiwal (Bos indicus) cows. J. Anim. Physiol. Anim. Nutr., 97: 315-321. [Crossref] [PubMed]
86. Patil, M.P., Nagvekar, A.S., Ingole, S.D., Bharucha, S.V. and Palve, V.T. (2015) Somatic cell count and alkaline phosphatase activity in milk for evaluation of mastitis in Buffalo. Vet. World, 8: 363-366. [Crossref] [PubMed] [PMC]
87. Fthenakis, G.C., El-Masannat, E.T.S., Booth, J.M. and Jones, J.E.T. (1991) Somatic cell counts of ewes' milk. Br. Vet. J., 147: 575-581. [Crossref]
88. Saleh, S.K. and Faye, B. (2011) Detection of subclinical mastitis in dromedary camels (Camelus dromedarius) using somatic cell counts, California mastitis test and udder pathogen. Emir. J. Food Agric., 23: 48-58. [Crossref]
89. Nagy, P., Faye, B., Marko, O., Thomas, S., Wernery, U. and Juhasz, J. (2013) Microbiological quality and somatic cell count in bulk milk of dromedary camels (Camelus dromedarius): Descriptive statistics, correlations, and factors of variation. J. Dairy Sci., 96: 5625-5640. [Crossref]
90. Kashongwe, O.B., Bebe, B.O., Matofari, J.W. and Huelsebusch, C.G. (2017) Associations between milking practices, somatic cell counts and milk postharvest losses in smallholder dairy and pastoral camel herds in Kenya. Int. J. Vet. Sci. Med., 5: 57-64. [Crossref]
91. Tancin, V., Baranovic, S., Uhrincat, M., Macuhova, L., Vrskova, M. and Oravcova, M. (2017) Somatic cell counts in raw ewes' milk in dairy practice: Frequency of distribution and possible effect on milk yield and composition. Mljekarstvo, 67: 253-260. [Crossref]
92. Jaeggi, J.J., Govindasamy-Lucey, S., Berger, Y.M., Johnson, M.E., McKusick, B.C., Thomas, D.L. and Wendorff, W.L. (2003) Hard ewe's milk cheese manufactured from milk of three different groups of somatic cell counts. J. Dairy Sci., 86: 3082-3089. [Crossref]
93. Albenzio, M., Santillo, A., Kelly, A.L., Caroprese, M., Marino, R. and Sevi, A. (2015) Activities of indigenous proteolytic enzymes in caprine milk of different somatic cell counts. J. Dairy Sci., 98: 7587-7594. [Crossref] [PubMed]
94. Silanikove, N., Merin, U., Shapiro, F. and Leitner, G. (2014) Subclinical mastitis in goats is associated with upregulation of nitric oxide-derived oxidative stress that causes reduction of milk antioxidative properties and impairment of its quality. J. Dairy Sci., 97: 3449-3455. [Crossref]
95. Packard, V.S. Jr., Tatini, R., Heady, F.J. and Gilman, C. (1992) Direct microscopic methods for bacteria or somatic cells. In: Marshall, R.T., editor. Standard Methods for the Examination of Dairy Products. 16th ed. American Public Health Association, Washington, DC, USA, p309-325.
96. Li, N., Richoux, R., Perruchot, M.H., Boutinaud, M., Mayol, J.F. and Gagnaire, V. (2015) Flow cytometry approach to quantify the viability of milk somatic cell counts after various physicochemical treatments. PLoS One, 10: e0146071. [Crossref] [PubMed] [PMC]
97. Syridion, D., Layek, S.S., Behera, K., Mohanty, T.K., Kumaresan, A., Manimaran, A., Dang, A.K. and Prasad, S. (2012) Effects of parity, season, stage of lactation, and milk yield on milk somatic cell count, pH and electrical conductivity in crossbred cows reared under subtropical climatic conditions. Milchwissenschaft, 67: 362-365.
98. Vissio, C., Bouman, M. and Larriestra, A.J. (2018) Milking machine and udder health management factors associated with bulk milk somatic cell count in Uruguayan herds. Prev. Vet. Med., 150: 110-116. [Crossref] [PubMed]
99. Dufour, S., Frechette, A., Barkema, H.W., Mussell, A. and Scholl, D.T. (2011) Invited review: Effect of udder health management practices on herd somatic cell count. J. Dairy Sci., 94: 563-579. [Crossref]
100. Emanuelson, U. and Nielsen, C. (2017) Weak associations between mastitis control measures and bulk milk somatic cell counts in Swedish dairy herds. J. Dairy Sci., 100: 6572-6576. [Crossref] [PubMed]
101. Wijga, S., Bastiaansen, J.W.M., Wall, E., Strandberg, E., De Haas, Y., Giblin, L. and Bovenhuis, H. (2012) Genomic associations with somatic cell score in first-lactation Holstein cows. J. Dairy Sci., 95: 899-908. [Crossref] [PubMed]
102. Sundekilde, U.K., Poulsen, N.A., Larsen, L.B. and Bertram, H.C. (2013) Nuclear magnetic resonance metabonomics reveals strong association between milk metabolites and somatic cell count in bovine milk. J. Dairy Sci., 96: 290-299. [Crossref] [PubMed]
103. Suarez-Vega, A., Toral, P.G., Gutierrez-Gil, B., Hervas, G., Arranz, J.J. and Frutos, P. (2017) Elucidating fish oil-induced milk fat depression in dairy sheep: Milk somatic cell transcriptome analysis. Sci. Rep., 7: 45905. [Crossref]
104. Li, S., Wang, Q., Lin, X., Jin, X., Liu, L., Wang, C., Chen, Q., Liu, J. and Liu, H. (2017) The use of "Omics" in lactation research in dairy cows. Int. J. Mol. Sci., 18: 983. [Crossref] [PubMed] [PMC]
105. Koeck, A., Miglior, F., Kelton, D.F. and Schenkel, F.S. (2012) Alternative somatic cell count traits to improve mastitis resistance in Canadian Holsteins. J. Dairy Sci., 95: 432-439. [Crossref] [PubMed]
106. De Jong, G. and Lansbergen, L.M.T.E. (1996) Udder health index: Selection for mastitis resistance. Proc., International Workshop on Genetic Improvement of Functional Traits in Cattle, Gembloux, Belgium. Interbull Bull., 12: 42-47.
107. Sorensen, M.K., Jensen, J. and Christensen, L.G. (2000) Udder conformation and mastitis resistance in Danish first-lactation cows: Heritabilities, genetic and environmental correlations. Acta Agric. Scand. A, 50: 72-82. [Crossref]
108. Martin, P., Barkema, H.W., Brito, L.F., Narayana, S.G. and Miglior, F. (2018) Symposium review: Novel strategies to genetically improve mastitis resistance in dairy cattle. J. Dairy Sci., 101: 2724-2736. [Crossref]
109. Leitner, G., Krifucks, O., Glickman, A., Vaadia, Y., Friedman, S., Ezra, E. and Trainin, Z. (2004) MASTIVAC I: Staphylococcus aureus vaccine-prevention of new udder infection and therapeutic effect on cows chronically infected with S. aureus under field conditions. Isr. J. Vet. Med., 59: 15-23.
111. Yang, M., Shi, J., Tian, J., Tao, J., Chai, M., Wang, J., Xu, Z., Song, Y., Zhu, K., Ji, P. and Liu, G. (2017) Exogenous melatonin reduces somatic cell count of milk in Holstein cows. Sci. Rep., 7: 43280. [Crossref] [PubMed] [PMC]
112. Yang, F.L. and Li, X.S. (2014) Role of antioxidant vitamins and trace elements in mastitis in dairy cows. J. Adv. Vet. Anim. Res., 2: 1-9. [Crossref]
113. Salami, S.A., Guinguina, A., Agboola, J.O., Omede, A.A., Agbonlahor, E.M. and Tayyab, U. (2016) In vivo and postmortem effects of feed antioxidants in livestock: A review of the implications on authorization of antioxidant feed additives. Animal, 10: 1375-1390. [Crossref] [PubMed]
114. Kruze, J., Ceballos, A., Stryhn, H., Mella, A., Matamoros, R., Contreras, P.A. and Wittwer, F. (2007) Somatic cell count in milk of selenium-supplemented dairy cows after an intramammary challenge with Staphylococcus aureus. Transbound. Emerg. Dis., 54: 478-483. [Crossref]
115. Malbe, M., Klaassen, M., Fang, W., Myllys, V., Vikerpuur, M., Nyholm, K. and Sandholm, M. (1995) Comparisons of selenite and selenium yeast feed supplements on Se-incorporation, mastitis and leucocyte function in Se-deficient dairy cows. Transbound. Emerg. Dis., 42: 111-121. [Crossref]
116. Mutoni, G., Prasad, S., De, K., Pal, S., Mukherjee, J., Kapila, S. and Dang, A.K. (2012) Effect of supplementation of vitamin E, copper and zinc around peripartum on udder health, milk yield and composition of Sahiwal cows. Livestock Res. Rur. Dev., 24: 220.
118. Castillo, C., Pereira, V., Abuelo, A. and Hernandez, J. (2013) Effect of supplementation with antioxidants on the quality of bovine milk and meat production. Sci. World J., 2013: 616098. [Crossref] [PubMed] [PMC]
119. Wang, Y.M., Wang, J.H., Wang, C., Wang, J.K., Chen, B., Liu, J.X. and Guo, F.C. (2010) Effect of dietary antioxidant and energy density on performance and anti-oxidative status of transition cows. Asian Aust. J. Anim. Sci., 23: 1299-1307. [Crossref]
120. Mardalena, M., Warly, L., Nurdin, E., Rusmana, W.S.N. and Farizal, F. (2011) Milk quality of dairy goat by giving feed supplement as antioxidant source. J. Indonesian Trop. Anim. Agric., 36: 205-212. [Crossref]
121. Panchal, I., Sawhney, I.K., Sharma, A.K. and Dang, A.K. (2016) Classification of healthy and mastitis Murrah buffaloes by application of neural network models using yield and milk quality parameters. Comput. Electron. Agric., 127: 242-248. [Crossref]
123. Jeretina, J., Skorjanc, D. and Babnik, D. (2017) A new somatic cell count index to more accurately predict milk yield losses. Arch. Tierz., 60: 373-383. [Crossref]
124. Varshney, N., Mohanty, A.K., Kumar, S., Kaushik, J.K., Dang, A.K., Mukesh, M. and Malakar, D. (2012) Selection of suitable reference genes for quantitative gene expression studies in milk somatic cells of lactating cows (Bos indicus). J. Dairy Sci., 95: 2935-2945. [Crossref]
125. Dang, A.K., Mukherjee, J., Kapila, S., Mohanty, A.K., Kapila, R. and Prasad, S. (2010) In vitro phagocytic activity of milk neutrophils during lactation cycle in Murrah buffaloes of different parity. J. Anim. Physiol. Anim. Nutr., 94: 706-711. [Crossref] [PubMed]
126. Jyotsana, B., Sahare, A.A., Raja, A.K., Singh, K.P., Singla, S.K., Chauhan, M.S., Manik, R.S. and Palta, P. (2015) Handmade cloned buffalo (Bubalus bubalis) embryos produced from somatic cells isolated from milk and ear skin differ in their developmental competence, epigenetic status, and gene expression. Cell. Reprogram., 17: 393-403. [Crossref]127. Boutinaud, M., Herve, L. and Lollivier, V. (2015) Mammary epithelial cells isolated from milk are a valuable, non-invasive source of mammary transcripts. Front. Genet., 6: 323. [Crossref] [PubMed] [PMC]