Open Access
Research (Published online: 29-05-2018)
22. The improvement of eggs quality of Mojosari duck (Anas javanica) with soybean husk fermentation using cellulolytic bacteria of Spodoptera litura
Sri Hidanah, Dady Soegianto Nazar and Erma Safitri
Veterinary World, 11(5): 720-725

Sri Hidanah: Department of Animal Husbandry, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia.
Dady Soegianto Nazar: Department of Animal Husbandry, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia.
Erma Safitri: Department of Veterinary Reproduction, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia; Stem Cells Research Division of Institute Tropical Disease (ITD), Universitas Airlangga, Surabaya, Indonesia.

doi: 10.14202/vetworld.2018.720-725

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Article history: Received: 06-03-2018, Accepted: 25-04-2018, Published online: 29-05-2018

Corresponding author: Erma Safitri

E-mail: rma_fispro@yahoo.com

Citation: Hidanah S, Nazar DS, Safitri E (2018) The improvement of eggs quality of Mojosari duck (Anas javanica) with soybean husk fermentation using cellulolytic bacteria of Spodoptera litura, Veterinary World, 11(5): 720-725.
Abstract

Aim: This study was aimed to improve the quality of the eggs of Mojosari duck (Anas javanica) through complete feeding containing soybean husk was fermented using cellulolytic bacteria of Spodoptera litura.

Materials and Methods: This study consisted of three stages: The first stages, isolation and identification of cellulolytic bacteria from S. litura; the second stage, the fermentation of soybean husk through the application of bacterial cellulolytic isolate from the first stage; and the third stage, the application of the best complete feed formulation from the second stage to Mojosari duck.

Results: There are four dominant bacteria: Bacillus sp., Cellulomonas sp., Pseudomonas sp., and Cytophaga sp. Furthermore, the best reduction of the crude fiber of soybean husks is the use of Cellulomonas sp. bacteria. The final of the study, the quality of the eggs of Anas javanica, was improved, as indicated by cholesterol decrease from the yolk without the decrease of egg weight and eggshell thickness, although the decrease in egg yolk color was inevitable.

Conclusion: Soy husk fermentation using cellulolytic bacteria of S. litura was added to complete feeding can be performed to improve the quality of the eggs of Mojosari duck.

Keywords: cellulolytic bacteria, eggs quality of duck, soybean husk fermentation, Spodoptera litura.

References

1. Sruamsiri, S., and Silman, P. (2008). Nutritive composition of soybean by-products and nutrient digestibility of soybean pod husk. Mj. Int. J. Sci. Tech. 2: 568-576.

2. Li, S., Zhu, D., Li, K., Yang, Y., Lei, Z. and Zhang, Z. (2013) Soybean curd residue: Composition, utilization, and related limiting factors. Int. Eng. Chem. Res. 13: 1-9. [Crossref]

3. Komatsu, K., Okuda, S., Takahashi, M. and Matsunaga, R. (2004) Antibiotic effect of insct-resistant soybean on common cutworm (Spodoptera litura) and its inheritance. Breeding Sci. 54: 27-32. [Crossref]

4. Thakur, A., Dhammi, P., Saini, H.S. and Kaur, S. (2015) Pathogenicity of bacteria isolated from gut of Spodoptera litura (Epidoptera: Noctuidae) and fitness costs of insect associated with consumption of bacteria. J. Invertebr. Pathol. 127: 38-46. [Crossref] [PubMed]

5. Eida, M.H., Nagaoka, T., Wasaki, J. and Kouno, K. (2012) Isolation and characterization of cellulose-decomposing bacteria inhabiting sawdust and coffee residue composts. Microb. Environ. 27: 226-233. [Crossref] [PMC]

6. Liang, Y.L., Zhang, Z., Wu, M., Wu, Y. and Feng, J.X. (2014) Isolation, screening, and identification of cellulolytic bacteria from natural reserves in the subtropical region of China and optimization of cellulose production by paenibacillus terrae ME27-1. Biomed. Res. Int. 19: 1-13.

7. Hong, T.T.T. and Ca, L. T. (2013) The protein content of cassava residue, soybean waste and rice bran is increased through fermentation with Aspergillus oryzae. Livestock Res. Rural Dev. 25: 1-7.

8. Jeyasankar, A., Raja, N. and Ignacimuthu, S. (2011) Insecticidal compound isolated from Syzygium lineare wall (Myrtaceae) against Spodoptera litura (Epidoptera: Noctuidae). Saudi J. Biol. Sci. 18: 329-332. [Crossref] [PubMed] [PMC]

9. Cakici, F.O., Sevim, A., Demirbag, Z. and Demir, I. (2014) Investigating internal bacteria of Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae) larvae and some Bacillus strains as biocontrol agents. Turk. J. Agric. For. 38: 99-110. [Crossref]

10. Yang, H.J., Park, S., Pak, V., Chung, K.R. and Kwon, D.Y. (2011) In: El-Shemy, H., editor. Fermented Soybean Products and Their Bioactive Compounds, Soybean and Health. In Tech, Republic of Korea. p535-538.

11. Dean, A.M. and Voss, D. (1999) Design and Analysis of Experiments. 1st ed. Springer, New York, NY, USA. p121-135. [Crossref]

12. Abell, L.L., Levy, B.B., Brodie, B.B. and Kendall, F.E. (1952) A simple method for the estimation of total cholesterol in serum and demonstration of its specificity. J. Biol. Chem. 195: 357-366.

13. Huang, T.C., Chen, C.P., Wefle, V. and Raftery, A. (1961) A stable reagent for the Liebermann-Burchard reaction. Application to rapid serum cholesterol determination. Anal. Chem., 33: 1405-1407. [Crossref]

14. Mikova, K. and Panovska, Z. (2014) Evaluation of egg yolk colour helena bovskova. Czech J. Food Sci. 32: 213-217. [Crossref]

15. Kibala, L., Rucinska, R., Kasperek, K., Zieba, G. and Lukaszewics, M. (2015) Ultrasonic eggshell thickness measurement for selection of layers. Poult. Sci. 94: 2360-2363. [Crossref] [PubMed]

16. Safitri, E., Utama, S., Widiyatno, T.V., Sandhika, W. and Prasetyo, R.H. (2016) Auto-regeneration of mice testicle seminiferous tubules due to malnutrition based on stem cells mobilization using honey. Asian Pac. J. Reprod. 5: 31-34. [Crossref]

17. Holt, J.G. (1964) Bergey's Manual of Determinative Bacteriology. 9th ed. Williams and Wilkins, New York, NY, USA. p57-79.

18. Gupta, P., Samant, K. and Sahu, A. (2012) Isolation of cellulose-degrading bacteria and determination of their cellulolytic potential. Int. J. Microbiol. 55: 1-5. [Crossref] [PubMed] [PMC]

19. Kasmirah, K., Fenita, Y. and Santoso, U. (2013) Effect of katuk (Sauropusandro gynus) Meal supplementation on egg cholesterol level of Mojosari duck (Anas javanica). Indones. Anim. Sci. J. 8: 77-86.

20. Palupi, R., Abdullah, L., Astuti, D. A. and Sumiati. (2014) High antioxidant egg production through substitution of soybean meal by Indigofera sp., Top leaf meal in laying hen diets. Int. J. Poult. Sci. 13: 198-203. [Crossref]

21. Subhan, A. (2014) Use of apple snails (Pomacea canaliculata) as a source of fatty acids in feed towards the performance, blood cholesterol and cholesterol levels in alabio duck (Anas platyrhinchos borneo) Meat and Eggs. Int. J. Poult. Sci. 13: 537-544. [Crossref]

22. Beardsworth, P.M. and Hernandez, J.M. (2004) Yolk color-an important egg quality attribute. Int. Poult. Prod. 12: 17-18.

23. Samik, A. and Safitri, E. (2017) Potency of mycotoxin binders on MDA level, expressions of caspase 9 and caspase 3 in the uterus of mice exposed to zearalenone. Iraqi. J. Vet. Sci. 31: 29-33.

24. Silversides, F.G. and Villeneuve, P. (1994) Is the haugh unit correction for egg seight valid for eggs stored at room temperature. J. Poult. Sci. 73: 50-55. [Crossref]

25. Samik, A. and Safitri, E. (2017) Mycotoxin binders potential on histological of ovary mice exposed by zearalenone. Vet. World, 10: 353-357. [Crossref] [PubMed] [PMC]

26. Prasetyo, R.H. and Safitri, E. (2016). Effects of honey to mobilize endogenous stem cells in efforts of intestinal and ovarian tissue regeneration in rats with protein-energy malnutrition. Asian Pac. J. Reprod., 5: 198-203. [Crossref]

27. Safitri, E., Widiyatno, T.V. and Prasetyo, R.H. (2016) Honeybee product therapeutic as stem cells homing for ovary failure. Vet. World, 9: 1324-1330. [Crossref] [PubMed] [PMC]

28. Prasetyo, R.H. and Hestianah, E.P. (2017) Honey can repair damage of liver tissue due to protein-energy malnutrition through induction of endogenous stem cells. Vet. World, 10: 711-715. [Crossref] [PubMed] [PMC]