Article history: Received: 17-07-2018, Accepted: 01-10-2018, Published online: 20-11-2018
Corresponding author: Om Prakash Dinani
E-mail: email@example.comCitation: Dinani OP, Tyagi PK, Mandal AB, Tyagi PK, Popat DS, Tiwari SP (2018) Effect of feeding rice based distillers dried grains with solubles and gluten meal on nutrient transporter genes and immunity in broiler chickens, Veterinary World, 11(11): 1592-1596.
Aim: The aim of this study was to investigate the effect of feeding rice based distillers dried grains with solubles (rDDGS) and gluten meal on nutrient transporter genes and immunity in broiler chickens.
Materials and Methods: A 2×3 factorial design resulted in six experimental diets, namely T1 (no rDDGS/rice gluten meal [RGM]/enzyme), T2 (no rDDGS/RGM, with multienzymes), T3 (12.5% rDDGS, 15% RGM, no enzyme), T4 (12.5% rDDGS, 15% RGM, with protease enzyme), T5 (10% rDDGS, 12.5% RGM, no enzyme), and T6 (10% rDDGS, 12.5% RGM, with protease enzyme). Each treatment was allocated five replicates of chicks, with eight birds in each. Nutrient transporter genes such as Mucin (MUC 2), excitatory amino acid transporter 3 (EAAT3), and peptide transporter (PepT1) and immunity were estimated using standard procedures.
Results: Feeding rDDGS and RGM combination improved humoral immunity, while cell-mediated immunity did not show any significant (p>0.05) effect on broiler chickens. MUC and PepT1 genes showed significantly (p<0.01) decreased relative fold expression in 12.5% rDDGS +15% RGM combination, while EAAT3 gene showed significantly (p<0.01) decreased relative fold expression in both rDDGS and RGM combination levels.
Conclusion: Thus, it may be concluded that feeding rDDGS and RGM combination improved humoral immunity but had an adverse effect on nutrient transporter gene in broiler chickens.
Keywords: broiler, immunity, nutrient transporter genes, protease, rice distillers dried grains with solubles, rice gluten meal.
1. ICAR. (2013) Nutrient Requirements of Animals-Poultry. ICAR-NIANP, New Delhi. p13-6.
2. Agricultural Statistics. (2015-16) Directorate of Economics and Statistics. Department of Agriculture, Cooperation and Farmers Welfare, Government of India, New Delhi.
3. AAFCO. (2005) Association of American Feed Control Officials. Available from: http://www.aafco.org . Last accessed on 08-12-2017.
4. Gilbert, E.R., Li, H., Emmerson, D.A., Webb, K.E. and Wong, E.A. (2008) Dietary protein quality and feed restriction influence abundance of nutrient transporter mRNA in the small intestine of broiler chicks. J. Nutr., 138(2): 262-271. [Crossref] [PubMed]
5. Gupta, S. (2016) Feeding value of rice-based dry distiller grains with soluble in white leghorn layers. Ph.D. Thesis Submitted to IVRIDU, Izatnagar, U.P.
6. Wani, M.A. (2017) Evaluation of Feeding Value of Rice Gluten Meal in Broiler Chicken. Ph.D. Thesis Submitted to IVRIDU, Izatnagar, U.P.
8. Siegel, P.B. and Gross, W.B. (1980) Production and persistence of antibodies in chickens to sheep erythrocytes. Poult. Sci. 59(1): 1-5. [Crossref]
10. Snedecor, G.W. and Cochran, W.G. (1989) Statistical Methods. 7th ed. Oxford and IBH, New Delhi.
11. Tukey, J. (1949) Comparing individual means in the analysis of variance. Biometrics, 5(2): 99-114. [Crossref]12. Barekataina, M.R., Antipatisb, C., Choct, M. and Ijia, P.A. (2013) Interaction between protease and xylanase in broiler chicken diets containing sorghum distillers' dried grains with soluble. Anim. Feed Sci. Technol., 182(1-4): 71-81. [Crossref]