Potential and safety tests of egg drop syndrome candidate vaccine from Medan isolate, Indonesia

Vet World Vol.11 November-2018 Article-18

Research Article

Veterinary World, 11(11): 1637-1640

https://doi.org/10.14202/vetworld.2018.1637-1640

Gusti Ayu Yuniati Kencana¹, Nyoman Suartha², I Made Kardena³, Gusti Ayu Mayani Kristina Dewi⁴, Arini Nurhandayani⁵, Syamsidar⁵, and Kadek Karang Agustina⁶

1. Department of Virology, Faculty of Veterinary Medicine, Udayana University, Denpasar, Indonesia.
2. Department of Internal Medicine, Faculty of Veterinary Medicine, Udayana University, Denpasar, Indonesia.
3. Department of Pathology, Faculty of Veterinary Medicine, Udayana University, Denpasar, Indonesia.
4. Department of Poultry Science, Faculty Animal Husbandry, Udayana University, Denpasar, Indonesia.
5. PT. Sanbio Laboratories, Bogor, Jawa Barat, Indonesia.
6. Department of Public Health, Faculty of Veterinary Medicine, Udayana University, Denpasar, Indonesia.

Background and Aim: The study was aimed to prepare and examine the potential and safety concerns of egg drop syndrome (EDS) vaccine candidate seed. The potential and safety trials of EDS Medan isolate vaccine need to be done before commercial scale of EDS vaccines are made.

Materials and Methods: The safety test of EDS candidate vaccine was tested on 4-week-old specified pathogen-free chickens in an experimentally isolated enclosure.

Results: The result of the safety test obtained 27.3 hemagglutination inhibition (HI) unit of geometric mean titer antibody post-vaccination. However, the potency test of the EDS candidate vaccine was conducted on 17-week-old laying hens. Test results of the EDS potency vaccine in layer obtained antibody titer increased in every week of blood taking with average titer of antibody: Before vaccinated was 22.9 HI unit, 1 week after vaccination was 23.7 HI unit, 2 weeks post-vaccination was 25 HI unit, and 3 weeks after vaccination was 27.3 HI units. In contrast, decreasing trend was observed in control group (unvaccinated chicken).

Conclusion: Serologically, the seed vaccine of EDS virus isolates from Medan was able to produce protective antibody titers starting in the 2nd and 3rd weeks post-vaccination. Keywords: hemagglutination, inhibition test, Medan isolate, safety and potential test, seed of egg drop syndrome vaccine.

Keywords: hemagglutination, inhibition test, Medan isolate, safety and potential test, seed of egg drop syndrome vaccine.

How to cite this article: Kencana GAY, Suartha N, Kardena IM, Dewi GAMK, Nurhandayani A, Syamsidar, Agustina KK (2018) Potential and safety tests of egg drop syndrome candidate vaccine from Medan isolate, Indonesia, Veterinary World, 11(11): 1637-1640.

Received: 03-05-2018 Accepted: 11-10-2018 Published online: 29-11-2018

Corresponding author: Gusti Ayu Yuniati Kencana E-mail: yuniati_kencana@unud.ac.id

DOI: 10.14202/vetworld.2018.1637-1640

Copyright: Kencana GAY, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.