Open Access
Research (Published online: 29-10-2018)
24. Genetic resistance of eight native Egyptian chicken breeds having chicken B-cell marker 6 gene post-challenge with field strain of Marek's disease-induced tumor virus
Hala A. Shaheen, H. A. Hussein, M. M. Elsafty and M. A. Shalaby
Veterinary World, 11(10): 1510-1515

Hala A. Shaheen: Central Laboratory for Evaluation of Veterinary Biologics, Cairo, Egypt.
H. A. Hussein: Department of Virology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.
M. M. Elsafty: Central Laboratory for Evaluation of Veterinary Biologics, Cairo, Egypt.
M. A. Shalaby: Department of Virology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.

doi: 10.14202/vetworld.2018.1510-1515

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Article history: Received: 08-07-2018, Accepted: 24-09-2018, Published online: 29-10-2018

Corresponding author: Hala A. Shaheen


Citation: Shaheen HA, Hussein HA, Elsafty MM, Shalaby MA (2018) Genetic resistance of eight native Egyptian chicken breeds having chicken B-cell marker 6 gene post-challenge with field strain of Marek's disease-induced tumor virus, Veterinary World, 11(10): 1510-1515.

Aim: The aim of this work was to detect chicken B-cell marker 6 (ChB6) gene in some native breeds in Egypt and find the relationship between founded genes in these different breeds to determine the resistance of native Egyptian breeds of chicken to Marek's disease (MD).

Materials and Methods: A total of 14 different chicken breeds (30 each) including ten native breeds in addition to SPF Lohmann, High Line, Bovans, and Roodiland were used. Blood samples were collected for the detection of (ChB6,) by polymerase chain reaction (PCR) assay and sequenced to determine the presence or absence of ChB6 gene. Experimental infection was done using local field isolated MD virus (MDV) of 11 (1 day old) unvaccinated chick breeds having no maternal antibodies against MDV. Ten breeds of them carry ChB6 gene, eight breeds were native, and the rest two breeds were SPF Lohmann and High Line in addition to a group of ChB6 gene-lacking breed (Bovans) were infected. Spleen samples were collected from all infected breeds at 20th, 25th, 30th, 35th, and 40th weeks post-infection and tested by PCR assay for the detection of MDV. Furthermore, at 40th week post-infection, tumorized spleen sample of Bovans breed was collected and prepared for examination by transmission electron microscope (TEM) to confirm the presence of MDV.

Results: Our results revealed the positivity of 10 out of 14 breeds (Gimmizah , Sinai, Dandarawi, Fayoumi, Golden Montazah, Matrouh, Beheri, Dokki, SPF Lohmann, and High Line) to the presence of ChB6 gene and resistance to MDV infection, while the Bovans, Mandarah , Inshas and Roodiland breeds lack the ChB6 gene and are susceptible to MDV infection. The collected spleen samples revealed negative for the presence of challenged MDV by PCR in 10 breeds (Gimmizah, Sinai, Dandarawi, Fayoumi, Golden Montazah, Matrouh, Beheri, Dokki, SPF Lohmann, and High Line) and positive for Bovans breed. TEM is used to confirm MDV infection in Bovans group which demonstrated tumors.

Conclusion: The study confirms the relationship between the presence of ChB6 gene in our native breeds and the absence of tumors.

Keywords: ChB6 gene, Egyptian chicken breeds, Marek's disease, polymerase chain reaction, transmission electron microscope.


1. OIE Terrestrial Manual. (2017) Marek's Disease. Ch. 2.3.13. World Assembly of Delegates of the OIE, Paris, France.

2. Denesvre, C. (2013) Marek's disease virus morphogenesis. Avian Dis., 57(2): 340-350. [Crossref] [PubMed]

3. McPherson, M.C. and Delany, M.E. (2016) Virus and host genomic, molecular, and cellular interactions during Marek's disease pathogenesis and oncogenesis. Poult. Sci., 95(2): 412-429. [Crossref] [PubMed] [PMC]

4. Witter, R.L. (1997) Increased virulence of Marek's disease virus field isolates. Avian Dis., 41(1):149-163. [Crossref]

5. Gilmour, D.G., Brand, A., Donnelly, N. and Stone, H.A. (1976) Bu-1 and Thy-1. Two loci determining surface antigens of B or T lymphocytes in the chicken. Immunogenetics, 3(1): 549-563. [Crossref]

6. Tregaskes, C.A., Bumstead, N., Davison, T.F. and Young, J.R. (1996) Chicken B-cell marker ChB6 (Bu-1) is a highly glycosylated protein of unique structure. Immunogenetics, 44(3): 212-217. [PubMed]

7. Bumstead, N. (1998) Genetic resistance to avian viruses. Rev. Sci. Tech. Off. Int. Epiz., 17(1): 249-255. [Crossref]

8. Minias, P., Bateson, W.Z., Whittingham, A.L., Johnson, A.J., McCance, S.O. and Dunn, O.P. (2018) Extensive shared polymorphism at non-MHC immune genes in recently diverged North American prairie grouse. Immunogenetics, 70(3): 195-204. [Crossref] [PubMed] [PMC]

9. Zhou, H. and Lamont, S.J. (2003) Associations of six candidate genes with antibody response kinetics in hens. Poult. Sci., 82(7): 1118-1126. [Crossref] [PubMed]

10. Reen, J.K., Sankhyan, V., Katoch, S. and Thakur, Y.P. (2013) Candidate gene polymorphism for IL-Rγ and ChB6 genes in the indigenous chicken of North Western Himalayan state of Himachal Pradesh, India. Poult. Sci. J., 1(2): 87-92.

11. National Center for Biotechnology Information. Available from: http://www.NCBI.GenBank. Last accessed on 25-10-2017.

12. Bioedit Software (Graphical and biological sequence editor) V 7.2.5 copyright © 1997-2013 Tom Hall.

13. MEGA 7 Align Software. V 7.0.21 copyright © 1993-2018 Koichiro Tamura, Glen Stecher and Sudhir Kumar.

14. Handberg, K.J., Nielsen, O.L. and J∅rgensen, P.H. (2001) The use of serotype 1-and serotype 3-specific polymerase chain reaction for the detection of Marek's disease virus in chickens. Avian Pathol., 30(3): 243-249. [Crossref] [PubMed]

15. Thornton, D.H. (1985) Quality control and standardization of vaccines. In: Payne, L.N., editor. Marek's Disease, Scientific Basis and Methods of Control. Martinus Nijhoff, Boston, MA. p267-291. [Crossref]

16. Gimeno, M.I. and Wakenell, S.P. (2016) Marek's Disease: A Laboratory Manual for the Isolation, Identification, and Characterization of Avian Pathogens. American Association of Avian Pathologists, Athens, Ga. p249-257.

17. Tischer, B.K., Schumacher, D., Beer, M., Beyer, J., Teifke, J.P., Osterrieder, K., Wink, K., Zelnik, V., Fehler, F. and Osterrieder, N. (2002) A DNA vaccine containing an infectious Marek's disease virus genome can confer protection against tumorigenic Marek's disease in chickens. J. Gen. Virol., 83(10): 2367-2376. [Crossref] [PubMed]

18. Schat, A.K. and Nair, V. (2013) Neoplastic disease (Marek's Disease). In: Disease of Poultry. 13th ed. Wiley-Blackwell, Danvers, MA. USA, p513-673.

19. Denesvre, C., Re'my, S., Trapp-Fragnet, L., Smith, L., Georgeault, P.S., Vautherot, J.F. and Nair, V. (2016) Marek's disease virus undergoes complete morphogenesis after reactivation in a T-lymphoblastoid cell line transformed by recombinant fluorescent marker virus. J. Gen. Virol., 97(2): 480-486. [Crossref] [PubMed] [PMC]

20. Soller, M. and Andersson, L. (1998) Genomic approaches to the improvement of disease resistance in farm animals. Rev. Sci. Technol., 17(1): 329-345. [Crossref]

21. Lamont, S.J. (1998) Impact of genetics on disease resistance. Poult. Sci., 77(8): 1111-1118. [Crossref] [PubMed]

22. Houssaint, E., Diez, E. and Pink, J.R. (1987) Ontogeny and tissue distribution of the chicken Bu-1a antigen. Immunology, 62(3): 463-470. [PubMed] [PMC]

23. Houssaint, E., Mansikka, A. and Vainio, O. (1991) Early separation of B and T lymphocyte precursors in chick embryo. J. Exp. Med., 174(2): 397-406. [Crossref]

24. Gilmour, D.G., Collins, W.M., Fredericksen, T.L., Urban, W.E., Ward, P.F. and DiFronzo, N.L. (1986) Genetic interaction between non-MHC T-and B-cell alloantigens in response to Rous sarcomas in chickens. Immunogenetics, 23(1): 1-6. [Crossref]

25. Taylor, R.L., Medarova, Z. and Briles, W.E. (2016) Immune effects of chicken non-MHC alloantigens. Poult. Sci., 95(2): 447-457. [Crossref] [PubMed]

26. Groenen, M.A., Cheng, H.H., Bumstead, N., Benkel, B.F., Briles, W.E., Burke, T., Burt, D.W., Crittenden, L.B., Dodgson, J., Hillel, J., Lamont, S., de Leon, A.P., Soller, M., Takahashi, H. and Vignal, A. (2000) A consensus linkage map of the chicken genome. Genome Res., 10(1): 137-147. [PubMed] [PMC]

27. O'Laughlin, J.P. (2010) Genetic Diversity of the ChB6 Alloantigen, College of Liberal Arts and Social Sciences Theses and Dissertations. Paper 18. Available from: Last accessed on 18-02-2018.

28. Bumstead, N., Sillibourne, J., Rennie, M., Ross, N. and Davison, F. (1997) Quantification of Marek's disease virus in chicken lymphocytes using the polymerase chain reaction with fluorescence detection. J. Virol. Methods, 65(1): 75-81. [Crossref]