Open Access
Research (Published online: 17-09-2018)
13. Dystroglycan 1: A new candidate gene for patellar luxation in Chihuahua dogs
Pattarawadee Srinarang, Korakot Nganvongpanit, Waranee Pradit, Kittisak Buddhachat, Puntita Siengdee, Kumpanart Soontornvipart and Siriwadee Chomdej
Veterinary World, 11(9): 1277-1284

Pattarawadee Srinarang: Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.
Korakot Nganvongpanit: Department of Veterinary Biosciences and Public Health, Animal Bone and Joint Research Laboratory, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, Thailand; Excellence Center in Osteology Research and Training Center, Chiang Mai University, Chiang Mai 50100, Thailand.
Waranee Pradit: Science and Technology Research Institute, Chiang Mai University, Chiang Mai 50200, Thailand.
Kittisak Buddhachat: Department of Biology, Faculty of Science, Naresuan University, Phitsanulok 65000, Thailand.
Puntita Siengdee: Department of Veterinary Biosciences and Public Health, Animal Bone and Joint Research Laboratory, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, Thailand.
Kumpanart Soontornvipart: Department of Veterinary Surgery, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand.
Siriwadee Chomdej: Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.

doi: 10.14202/vetworld.2018.1277-1284

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Article history: Received: 30-04-2018, Accepted: 02-08-2018, Published online: 17-09-2018

Corresponding author: Siriwadee Chomdej


Citation: Srinarang P, Nganvongpanit K, Pradit W, Buddhachat K, Siengdee P, Soontornvipart K, Chomdej S (2018) Dystroglycan 1: A new candidate gene for patellar luxation in Chihuahua dogs, Veterinary World, 11(9): 1277-1284.

Aim: The objective of this study was to uncover new candidate genes related to patellar luxation (PL) in dogs to select for those with low susceptibility for breeding purposes.

Materials and Methods: The inter simple sequence repeat (ISSR) technique was performed to construct DNA fingerprints of 61 Chihuahua dogs with PL and 30 healthy Chihuahua dogs. DNA polymorphisms were detected by comparing the sequences between the affected and unaffected dogs, using the pairwise alignments in MultAlin. Genotyping was performed using allele-specific polymerase chain reaction (AS-PCR). The association analysis of ISSR DNA fingerprints and genotypes or phenotypes was performed using the Chi-square (χ2) model and generalized linear model (GLM), respectively.

Results: Two single nucleotide polymorphisms (SNPs), namely SNP1UBC811 (g.91175C>G) and SNP2UBC811 (g.92259T>C), were found in the intron of the Dystroglycan 1 (DAG1) gene, which was obtained using the PL-related marker UBC811 primer (p=0.02), and genotyped by AS-PCR. When investigated using the GLM, g.91175C>G had a significant association with PL (p=0.0424), whereas g.92259T>C did not have such an association (p=0.0959).

Conclusion: DAG1 might be one of the genes related to PL in Chihuahuas and could aid the process of marker-assisted selection in genetic breeding for Chihuahua dogs without PL.

Keywords: DNA marker, Dystroglycan 1 gene, inter simple sequence repeat, patellar luxation, single-nucleotide polymorphism.


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