Dystroglycan 1: A new candidate gene for patellar luxation in Chihuahua dogs

Vet World Vol.11 September-2018 Article-13

Research Article

Veterinary World, 11(9): 1277-1284

https://doi.org/10.14202/vetworld.2018.1277-1284

Pattarawadee Srinarang¹, Korakot Nganvongpanit^2,3, Waranee Pradit⁴, Kittisak Buddhachat⁵, Puntita Siengdee², Kumpanart Soontornvipart⁶, and Siriwadee Chomdej¹

1. Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.
2. Department of Veterinary Biosciences and Public Health, Animal Bone and Joint Research Laboratory, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, Thailand.
3. Excellence Center in Osteology Research and Training Center, Chiang Mai University, Chiang Mai 50100, Thailand.
4. Science and Technology Research Institute, Chiang Mai University, Chiang Mai 50200, Thailand.
5. Department of Biology, Faculty of Science, Naresuan University, Phitsanulok 65000, Thailand.
6. Department of Veterinary Surgery, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand.

Background and Aim: The objective of this study was to uncover new candidate genes related to patellar luxation (PL) in dogs to select for those with low susceptibility for breeding purposes.

Materials and Methods: The inter simple sequence repeat (ISSR) technique was performed to construct DNA fingerprints of 61 Chihuahua dogs with PL and 30 healthy Chihuahua dogs. DNA polymorphisms were detected by comparing the sequences between the affected and unaffected dogs, using the pairwise alignments in MultAlin. Genotyping was performed using allele-specific polymerase chain reaction (AS-PCR). The association analysis of ISSR DNA fingerprints and genotypes or phenotypes was performed using the Chi-square (χ2) model and generalized linear model (GLM), respectively.

Results: Two single nucleotide polymorphisms (SNPs), namely SNP1UBC811 (g.91175C>G) and SNP2UBC811 (g.92259T>C), were found in the intron of the Dystroglycan 1 (DAG1) gene, which was obtained using the PL-related marker UBC811 primer (p=0.02), and genotyped by AS-PCR. When investigated using the GLM, g.91175C>G had a significant association with PL (p=0.0424), whereas g.92259T>C did not have such an association (p=0.0959).

Conclusion: DAG1 might be one of the genes related to PL in Chihuahuas and could aid the process of marker-assisted selection in genetic breeding for Chihuahua dogs without PL. Keywords: DNA marker, Dystroglycan 1 gene, inter simple sequence repeat, patellar luxation, single-nucleotide polymorphism.

Keywords: DNA marker, Dystroglycan 1 gene, inter simple sequence repeat, patellar luxation, single-nucleotide polymorphism.

How to cite this article: Srinarang P, Nganvongpanit K, Pradit W, Buddhachat K, Siengdee P, Soontornvipart K, Chomdej S (2018) Dystroglycan 1: A new candidate gene for patellar luxation in Chihuahua dogs, Veterinary World, 11(9): 1277-1284.

Received: 30-04-2018 Accepted: 02-08-2018 Published online: 17-09-2018

Corresponding author: Siriwadee Chomdej E-mail: siriwadee.submission@gmail.com

DOI: 10.14202/vetworld.2018.1277-1284

Copyright: Srinarang, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.