Open Access
Research (Published online: 01-04-2019)
1. Sumateran wild boar (Sus scrofa vittatus) meat antibody production as immunodiagnostic reagent candidate
Melani Wahyu Adiningsih, Retno Damajanti Soejoedono, Rahmat Setya Adji, Dwi Desmiyeni Putri, Trioso Purnawarman, Hadri Latif and Okti Nadia Poetri
Veterinary World, 12(4): 477-482

Melani Wahyu Adiningsih: Study Program of Veterinary Public Health, IPB Graduate School, Bogor Agricultural University, Indonesia; Indonesia Agricultural Quarantine Agency, Indonesia.
Retno Damajanti Soejoedono: Department of Animal Diseases and Veterinary Public Health, Faculty of Veterinary Medicine, Bogor Agricultural University, Indonesia.
Rahmat Setya Adji: Center of Veterinary Research, Bogor, Indonesia.
Dwi Desmiyeni Putri: Department of Animal Husbandry, Faculty of Animal Husbandry, State Polytechnic of Lampung, Indonesia.
Trioso Purnawarman: Department of Animal Diseases and Veterinary Public Health, Faculty of Veterinary Medicine, Bogor Agricultural University, Indonesia.
Hadri Latif: Department of Animal Diseases and Veterinary Public Health, Faculty of Veterinary Medicine, Bogor Agricultural University, Indonesia.
Okti Nadia Poetri: Department of Animal Diseases and Veterinary Public Health, Faculty of Veterinary Medicine, Bogor Agricultural University, Indonesia.

doi: 10.14202/vetworld.2019.477-482

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Article history: Received: 17-10-2018, Accepted: 14-02-2019, Published online: 01-04-2019

Corresponding author: Retno Damajanti Soejoedono


Citation: Adiningsih MW, Soejoedono RD, Adji RS, Putri DD, Purnawarman T, Latif H, Poetri ON (2019) Sumateran wild boar (Sus scrofa vittatus) meat antibody production as immunodiagnostic reagent candidate. Veterinary World, 12(4): 477-482.

Aim: Meat authentication gives significance values in view of religious, food safety, public health, quality assurance, and legal concern. Most of the meat authentication is based on molecular assay; a simpler method to authenticate meat is needed to develop. An immunoassays technique may offer a solution for simpler test. The aim of our current study was to develop a polyclonal antibody of Sus scrofa vittatus (Sumateran wild boar) as an immunodiagnostic reagent candidate.

Materials and Methods: Three male New Zealand white rabbits were used in this study for antibody production. Antigen used was meat extract of Sumateran wild boar, each rabbit was immunized with meat extract antigen (0.5 mg/ml) emulsified in Freund's complete adjuvant at a 1:1 (v/v) ratio as much as 1 ml at subcutaneous route. Booster was carried out 3 times with interval time of 14 days, using meat extract antigen emulsified in Freund's incomplete adjuvant at a 1:1 (v/v) ratio. Serum samples were taken every week, start from 1 week after the first immunization up to 1 week after the third booster. Antibody purification was performed using ammonium sulfate precipitation and Protein A. The presence of specific antibody was determined using agar gel precipitation test and enzyme-linked immunosorbent assay, while purified specific IgG was characterized using sodium dodecyl sulfate-polyacrylamide gel electrophoresis method.

Results: Specific antibody was detected at 14 days after the first immunization and still detected until 2 weeks after the third booster. Highest absorbance of specific antibody was detected 1 week after the third booster.

Conclusion: The present study demonstrated that specific antibody of Sumateran wild boar is favorable to be produced in rabbit and showed that antibody produced is applicable to detect Sumateran wild boar meat antigen in immunodiffusion assay, indicating that it is promising as a reagent candidate in immunodiagnostic assay/kit.

Keywords: antibody, enzyme-linked immunosorbent assay, rabbit, reagent, Sumateran wild boar.


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