doi: 10.14202/vetworld.2019.558-564
Share this article on [Facebook] [LinkedIn]
Article history: Received: 05-11-2018, Accepted: 20-02-2019, Published online: 18-04-2019
Corresponding author: Xichun Wang
E-mail: wangxichun@ahau.edu.cn
Citation: Rahman SU, Zhu L, Cao L, Zhang Y, Chu X, Feng S, Li Y, Wu J, Wang X (2019) Prevalence of Caprine brucellosis in Anhui province, China, Veterinary World, 12(4): 558-564.Background and Aim: Brucellosis is one of the most widespread zoonotic diseases globally. Studies indicated the existence of Brucella infection in goats in some province of China. Thus this study aimed to estimate the prevalence of brucellosis in goats of Anhui Province, China.
Materials and Methods: Serum and milk samples obtained from goats in different regions of Anhui province were studied through rose Bengal plate test (RBPT), serum agglutination test (SAT), milk ring test (MRT), and polymerase chain reaction (PCR).
Results: The investigation frequency of brucellosis using RBPT, SAT, MRT, and PCR methods was 3.9% (n=7), 4.45% (n=8), 11.67% (n=7), and 86.67% (n=156), respectively. The prevalence recorded for brucellosis in sex-wise animals as in females 5.55%, 6.67%, 11.67%, and 78.8% through above methods, while in males, it was 2.23% and 2.23% by RBPT and SAT. However, in age-wise animals, the results 6.36%, 7.27%, 11.67%, and 74.5% were perceived positive by RBPT, SAT, MRT, and PCR in adult females, respectively, but young males and females (up to 9 months) were considered free from brucellosis.
Conclusion: These results show that prevalence of brucellosis was relatively higher in females than male's goats and SAT was relatively specific and accurate as compared to RBPT and MRT, but for diagnosis of brucellosis, molecular method (PCR) is recommended.
Keywords: brucellosis, goats, polymerase chain reaction, prevalence, serological tests.
1. Gwida, M., Dahouk, S.A., Melzer, F., Rosler, U., Neubauer, H. and Tomaso, H. (2010) Brucellosis regionally emerging zoonotic disease? Croat. Med. J., 51(4): 289-295. [Crossref]
2. Dahouk, A.S., Sprague, L.D. and Neubauer, H. (2013) New developments in the diagnostic procedures for zoonotic brucellosis in humans. Rev. Sci. Tech., 32(1): 177-188. [Crossref]
3. Luikart, G., Gielly, L., Excoffier, L., Vigne, J.D., Bouvet, J. and Taberlet, P. (2001) Multiple maternal origins and weak phylogeographic structure in domestic goats. Proc. Natl. Acad. Sci. U. S. A., 98(10): 5927-5932. [Crossref] [PubMed] [PMC]
4. Man, T.F., Wang, D.L., Cui, B.Y., Wang, Y., Ding, F., Li, T.F., Sun, H., Wang, R.J., Li, Q. and Feng, Z.J. (2010) Ana1ysis on survei11ance data of bruce11osis in China. Dis. Surveill., 25(12): 944-946.
5. Chen, G.C., Liu, Z.B., Gong, X.J., Wang, D.M., Wang, Y., Wang, S.X. and Huang, Y.P. (2011) Analysis of surveillance results of brucellosis in human being in Guizhou province from 2005 to 2008. Chin. J. Endemiol., 30(6): 681-683.
6. Li, S.C., Li, Y.S., Zhu, Q.L. and Wang, H.W. (2012) Surveillance and analysis of brucellosis from 2005 to 2011 in Baiyin distinct. Chin. J. Control Endem. Dis., 27(5): 368.
7. Baek, B.K., Lim, C.W., Rahman, M.S., Kim, C.H., Oluoch, A. and Kakoma, I. (2003) Brucella abortus infection in indigenous Korean dogs. Can. J. Vet. Res., 67(4): 312.
8. Kakoma, I., Oluoch, A.O., Baek, B.K., Rahman, M.S., Matsuda, K. (2003) More attention warranted on Brucella abortus in animals. J. Am. Vet. Med. Assoc., 222(3): 284.
9. Shapoury, R., Sattari, M. and Hassan, Z.M. (2006) Studies on the antimicrobial effect of allicin on the intra macrophages Brucella. Pak. J. Biol. Sci., 9(10): 1935-1939. [Crossref]
10. Deghelt, M., Mullier, C., Sternon, J.F., Francis, N., Laloux, G., Dotreppe, D., Henst, C.V.D., Jacobswagner, C., Letesson, J.J. and Bolle, X.D. (2014) G1-arrested newborn cells are the predominant infectious form of the pathogen Brucella abortus. Nat. Commun., 5(5): 4366. [Crossref]
11. Singh, B.B., Dhand, N.K. and Gill, J.P. (2015) Economic losses occurring due to brucellosis in Indian livestock populations. Prev. Vet. Med., 119(3-4): 211-215. [Crossref] [PubMed]
12. Corbel, M.J. (2006) Brucellosis in Human and Animals. World Health Organization Geneva Switzerland. Available from: http://www.who.int/iris/handle/10665/43597. Last accessed on 03-04-2019.
13. Pappas, G., Akritidis, N., Bosilkovski, M. and Tsianos, E. (2005) Brucellosis. N. Engl. J. Med., 352(22): 2325. [Crossref]
14. Kushwaha, N., Rajora, V.S., Mohan, A., Upadhyay, A.K. and Kumar, R. (2016) Comparison of serological tests for detection of Brucella antibodies in cattle of an organized dairy farm. Indian J. Anim. Res., 50(1): 69-74. [Crossref]
15. Ahasan, M.S., Rahman, M.S., Rahman, A.K. and Berkvens, D. (2017) Bovine and caprine brucellosis in Bangladesh: Bayesian evaluation of four serological tests, true prevalence, and associated risk factors in household animals. Trop. Anim. Health Prod., 49(1): 1-11. [Crossref]
16. Godfroid, J., Saegerman, C., Wellemans, V., Walravens, K., Letesson, J.J., Tibor, A., Mc Millan, A., Spencer, S., Sanna, M., Bakker, D. and Pouillot, R. (2002) How to substantiate eradication of bovine brucellosis when a specific serological reactions occur in the course of brucellosis testing. Vet. Microbiol., 90(1-4): 461-477. [Crossref]
17. Wareth, G., Melzer, F., Tomaso, H., Roesler, U. and Neubauer, H. (2015) Detection of Brucella abortus DNA in aborted goats and sheep in Egypt by real-time PCR. BMC Res. Notes, 8(1): 212. [Crossref]
18. Nielsen, K. (2002) Diagnosis of brucellosis by serology. Vet. Microbiol., 90(1): 447-459. [Crossref]
19. Gupta, V.K., Verma, D.K., Rout, P.K., Singh, S.V. and Vihan, V.S. (2005) Polymerase chain reaction (PCR) for detection of Brucella melitensis in goat milk. Small Rumin. Res., 65(1): 79-84. [Crossref]
20. Shahaza, O., Khairani-Bejo, S., Zunita, Z. and Bahaman, A.R. (2009) In-house rose bengal plate agglutination test (RBPT) for rapid diagnosis of brucellosis in goats in Malaysia. Int. J. Trop. Med., 4(3): 11-116.
21. Islam, M.A., Samad, M.A. and Rahman, M.A. (2010) Risk factor associated with prevalence of brucellosis in black Bengal goats in Bangladesh. Bangladesh J. Vet. Med., 8(2): 141-147. [Crossref]
22. Khan, T.S., Ehtisham-ul-Haque, S., Waheed, U., Khan, I., Younas, M. and Ali, S. (2018) Milk indirect-ELISA and milk ring test for screening of brucellosis in buffaloes, goats and bulk tank milk samples collected from two districts of Punjab, Pakistan. Pak. Vet. J., 38(1): 105-108. [Crossref]
23. Alton, G.G., Jones, L.M., Angus, R.D. and Verger, J.M. (1988) Techniques for the Brucellosis Laboratory. Institut National de la Recherche Agronomique (INRA), Paris.
24. OIE. (2009) Brucellosis. OIE Technical Manual, World Organization for Animal Health, Available from: http://www.oie.int/en/animal-health-in-the-world/animal-diseases/Brucellosis. Last accessed on 03-04-2019.
25. Zhong, Z., Xu, X., Li, X., Liu, S., Lei, S., Yang, M., Yu, J., Yuan, J., Ke, Y. and Du, X. (2016) Large-scale identification of small noncoding RNA with strand-specific deep sequencing and characterization of a novel virulence-related sRNA in Brucella melitensis. Sci. Rep., 6: 25123. [Crossref]
26. Saadat, S., Mardaneh, J., Ahouran, M., Mohammadzadeh, A., Ardebili, A., Yousefi, M. and Mansouri, M. (2017) Diagnosis of cattle brucellosis by PCR and serological methods: Comparison of diagnostic tests. Biomed. Pharm. J., 10(2): 881-888. [Crossref]
27. Esendal, O.M., Yardimc, H., Keski?n, O. and Altay, G. (2001) The use of conventional tests and coombs test in the serological diagnosis of bovine, ovine and caprine brucellosis. Ankara Univ. Vet. Fak., 48(1): 97-102.
28. Bale, J.O.O., Nuru, S., Addo, P.B. and Adeyinka, I.A. (2003) Bacteriological investigation of sheep and goat milk for brucellosis in government farms in Northern Nigeria. Niger. J. Anim. Prod., 30(1): 6. [Crossref]
29. Nagati, S.F. and Hassan, S.K. (2016) Diagnosis of Brucella infection in sheep and goat and evaluation of the associated practices in animal contacts. Am. J. Infect. Dis. Microbiol., 4(5): 95-101.
30. Al-Majali, A.M., Talafha, A.Q., Ababneh, M.M. and Ababneh, M.M. (2009) Seroprevalence and risk factors for bovine brucellosis in Jordan. J. Vet. Sci., 10(1): 61-65. [Crossref] [PMC]
31. Chandra, M., Singh, B.R., Harishankar., Agarwal, M., Agarwal, R., Sharma, G. and Babu, N. (2005) Seroprevalence of brucellosis in chevon goats from Bareilly slaughterhouse. Indian J. Anim. Sci., 75(2): 220-221.
32. Akhtar, R., Chaudhry, Z.I., Shakoori, A.R., Ahmad, M.D. and Aslam, A. (2010) Comparative efficacy of conventional diagnostic methods and evaluation of polymerase chain reaction for the diagnosis of bovine brucellosis. Vet. World, 3(2): 53-56.
33. Grewal, S.S. and Kaur, S. (2000) Survey of sheep and goat brucellosis in Sangrur district (Punjab, India). J. Parasitol. Appl. Anim. Biol., 9(2): 67-74.
34. Hashemi, S.H., Torkaman, A.F., Alikhani, M.Y. and Naseri, Z. (2015) Comparison of culture and serological methods for the diagnosis of brucellosis. Sci. J. Hamadan Univ. Med. Sci., 22(1): 37-42.
35. El-Diasty, M., Wareth, G., Melzer, F., Mustafa, S., Sprague, L.D. and Neubauer, H. (2018) Isolation of Brucella abortus and Brucella melitensis from seronegative cows is a serious impediment in brucellosis control. Vet. Sci., 5(1): E28. [Crossref]