Open Access
Research (Published online: 02-04-2019)
2. Effect of citrus waste on blood parameters of broiler birds with and without cocktail of enzymes
Devi Prasad Behera, Amrit Pal Singh Sethi, Chanchal Singh, Udeybir Singh and Manju Wadhwa
Veterinary World, 12(4): 483-488

Devi Prasad Behera: Department of Animal Nutrition, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, India.
Amrit Pal Singh Sethi: Department of Animal Nutrition, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, India.
Chanchal Singh: Department of Veterinary Biochemistry, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, India.
Udeybir Singh: Department of Animal Nutrition, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, India.
Manju Wadhwa: Department of Animal Nutrition, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, India.

doi: 10.14202/vetworld.2019.483-488

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Article history: Received: 24-10-2018, Accepted: 29-01-2019, Published online: 02-04-2019

Corresponding author: Amrit Pal Singh Sethi

E-mail: apss_pau_ldh@yahoo.co.in

Citation: Behera DP, Sethi APS, Singh C, Singh U, Wadhwa M (2019) Effect of citrus waste on blood parameters of broiler birds with and without cocktail of enzymes, Veterinary World, 12(4):483-488.
Abstract

Aim: This study aimed to assess the effect of different levels of citrus waste (CW) with or without enzyme cocktail on blood profile of broilers.

Materials and Methods: CW was sun-dried and grounded to powder CW. 256-day-old birds were distributed into eight groups; control (C), CW-supplemented diets (2.5% CW, 5.0% CW, and 7.5% CW), enzyme (E) cocktail supplemented diets (CE, 2.5% CWE, 5.0% CWE, and 7.5% CWE). The diets were fed during starter (0-14 days), grower (15-21 days), and finisher (22-42 days) phases. Blood was collected from the wing vein from four birds per treatment. Serum was separated out after centrifugation and stored at −20°C until further analysis. The samples were analyzed for liver function test (glucose, total protein [TP], albumin [ALB], and globulin), lipid profile (cholesterol and triglyceride), kidney function test (alanine aminotransferase, aspartate aminotransferase [AST], blood urea nitrogen [BUN], and creatinine), and antioxidant levels (catalase, superoxide dismutase [SOD], lipid peroxidation [LPx], glutathione peroxidase [GPx], glutathione [GSH], and Vitamins E and C).

Results: Blood profile data revealed that supplementation of CW showed no effect on TP, ALB, globulin, and BUN levels. Plasma cholesterol, triglyceride, and AST levels decreased linearly with an increase in the levels of CW in the diet. Catalase and SOD activity increased non-significantly with an increase in inclusion level of CW in the diets. LPx, GPx, and GSH activities decreased (p≤0.05) up to 5% CW-fed groups. Vitamin E and C activity were found to be highest (p≤0.05) in birds fed with diet supplemented with 5% CW. GPx and GSH activities and serum Vitamin C levels were observed to be highest (p<0.05) in birds fed CW (at 5%)-based diet supplemented with enzymes.

Conclusion: The blood profile showed that supplementation of CW up to 5% decreased cholesterol, triglyceride, and AST levels and improved the antioxidant status. Vitamin C levels were observed to be highest (p<0.05) in birds fed CW (at 5%)- based diet supplemented with enzymes.

Keywords: antioxidant levels, citrus waste, cocktail of enzymes, lipid profile, liver and kidney function test.

References

1. Thirumalaisamy, G., Muralidharan, J., Senthilkumar, S., Hema, S.R. and Priyadharsini, M. (2016) Cost-effective feeding of poultry. Int. J. Sci. Environ. Technol., 5(6): 3997-4005.

2. Wadhwa, M. and Bakshi, M.P.S. (2013) Utilization of Fruit and Vegetable Wastes as Livestock Feed and as Substrates for Generation of Other Value-Added Products. 1st ed. RAP Publication, Bangkok, Thailand.

3. Crawshaw, R. (2004) Co-Product Feeds: Animal Feeds from the Food and Drinks Industries. Nottingham University Press, Nottingham.

4. Nobakht, A. (2013) Effects of different levels of dried lemon (Citrus aurantifulia) pulp on performance, carcass traits, blood biochemical and immunity parameters of broilers. Iran. J. Appl. Anim. Sci., 3(1): 145-151.

5. Iskender, H., Yenice, G., Dokumacioglu, E., Kaynar, O., Hayirli, A. and Kaya, A. (2016) The effects of dietary flavonoid supplementation on the antioxidant status of laying hens. Braz. J. Poult. Sci., 18(4): 663-668. [Crossref]

6. Nazok, A., Rezaei, M. and Sayyahzadeh, H. (2010) Effect of different levels of dried citrus pulp on performance, egg quality, and blood parameters of laying hens in early phase of production. Trop. Anim. Health. Prod., 42(4): 737-742. [Crossref] [PubMed]

7. AOAC. (2000) Official Methods of Analysis. 16th ed. Association of Official Analytical Chemist, Washington, DC.

8. Talapatra, S.K., Roy, S.C. and Sen, K.C. (1940) Estimation of phosphorus, chlorine, calcium, sodium and potassium in foodstuffs. Ind. J. Vet. Sci. Anim. Husbandry, 10: 243-258.

9. ICAR. (2013) Nutrient Requirement of Animals Poultry (ICAR-NIANP). 3rd ed. Krishi Bhawan, New Delhi.

10. SPSS. (2016) Statistical Packages for Social Sciences Version 24.0. SPSS Inc., Chicago, USA.

11. Duncan, D.B. (1955) Multiple range and multiple F-tests. Biometrics, 11(1): 1-42. [Crossref]

12. Hajati, H., Hassanabadi, A. and Yansari, A.T. (2012) Effect of Citrus Pulp on Performance and Some Blood Parameters of Broiler Chickens. 1st International and 4th National Congress on Recycling of Organic Waste in Agriculture.

13. Bahman, A.H., Alireza, T. and Siamak, A.R. (2011) Biochemical profile of chicken. Glob. Vet., 7(3): 238-241.

14. Akbarian, A., Golian, A., Gilani, H., Kermanshahi, H., Zhaleh, S., Akhavan, A., Smet, S.D. and Michiels, J. (2013) Effect of feeding citrus peel extracts on growth performance, serum components and intestinal morphology of broilers exposed to high ambient temperature during the finisher phase. Livest. Sci., 157(2-3): 490-497. [Crossref]

15. Abdel-Moneim, M.A., Hamady, G.A.A. and Motawe, H.F.A. (2014) The use of orange waste with and without enzymes in broilers' diets and its effect on their performance, carcass traits and some blood parameters. Res. J. Anim. Vet. Fish. Sci., 2(12): 14-19.

16. Abbasi, H., Seidavi, A., Liu, W. and Asadpour, L. (2015) Investigation on the effect of different levels of dried sweet orange (Citrus sinensis) pulp on performance, carcass characteristics and physiological and biochemical parameters in broiler chicken. Saudi J. Biol. Sci., 22(2): 139-146. [Crossref] [PubMed] [PMC]

17. Ebrahimi, A., Qotbi, A.A.A., Seidavi, A., Edens, F.W., Laudadio, V. and Tufarelli, V. (2016) Selected plasma constituents of broiler chickens fed different levels of dried sweet orange (Citrus sinensis) peels. J. Anim. Plant Sci., 26(4): 949-955.

18. Dutta, S.K. and Hlasko, J. (1985) Dietary fibre in pancreatic disease: Effect of high fibre on fat malabsorption in pancreatic insufficiency and in vitro study of the interaction of dietary fibre with pancreatic enzymes. Am. J. Clin. Nutr., 41(3): 517-525. [Crossref] [PubMed]

19. Faiz, F., Khan, M.I., Sadiq, M. and Nawaz, H. (2017) Effects of dietary natural antioxidants from citrus waste on growth and blood antioxidants status of the broilers. Sarhad J. Agric., 33(3): 371-376. [Crossref]

20. Trovato, A., Monforte, M.T., Barbera, R., Rossitto., Galati, A.E.M. and Forestieri, A.M. (1996) Effects of fruit juices of Citrus sinensis L. and Citrus limon L. on experimental hypercholesterolemia in the rat. Phytomedicine, 2(3): 221-2267. [Crossref]

21. Parmar, H.S. and Kar, A. (2008) Antiperoxidative, antithyroidal, antihyperglycemic and cardioprotective role of Citrus sinensis peel extract in male mice. Phytother. Res., 22(6): 791-795. [Crossref] [PubMed]