Open Access
Research (Published online: 25-08-2019)
21. In vitro study of ivermectin efficiency against the cattle tick, Rhipicephalus (Boophilus) annulatus, among cattle herds in El-Beheira, Egypt
Gaber E. Batiha, Ali H. El-Far, Amany A. El-Mleeh, Abdelwahab A. Alsenosy, Eman K. Abdelsamei, Mohamed M. Abdel-Daim, Yasser S. El-Sayed and Hazem M. Shaheen
Veterinary World, 12(8): 1319-1326

Gaber E. Batiha: Department of Pharmacology, Faculty of Veterinary Medicine, Damanhour University, Egypt.
Ali H. El-Far: Department of Biochemistry, Faculty of Veterinary Medicine, Damanhour University, Egypt.
Amany A. El-Mleeh: Department of Pharmacology, Faculty of Veterinary Medicine, Menoufia University, Egypt.
Abdelwahab A. Alsenosy: Department of Biochemistry, Faculty of Veterinary Medicine, Damanhour University, Egypt.
Eman K. Abdelsamei: Department of Parasitology, Faculty of Veterinary Medicine, Menoufia University, Egypt.
Mohamed M. Abdel-Daim: Department of Pharmacology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia 41522, Egypt.
Yasser S. El-Sayed: Department of Veterinary Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Damanhour University, Egypt.
Hazem M. Shaheen: Department of Pharmacology, Faculty of Veterinary Medicine, Damanhour University, Egypt.

doi: 10.14202/vetworld.2019.1319-1326

Share this article on [Facebook] [LinkedIn]

Article history: Received: 31-03-2019, Accepted: 08-07-2019, Published online: 25-08-2019

Corresponding author: Hazem M. Shaheen

E-mail: dr_hazemshaheen3010@yahoo.com

Citation: Batiha GE, El-Far AH, El-Mleeh AA, Alsenosy AA, Abdelsamei EK, Abdel-Daim MM, El-Sayed YS, Shaheen HM (2019) In vitro study of ivermectin efficiency against the cattle tick, Rhipicephalus (Boophilus) annulatus, among cattle herds in El-Beheira Egypt, Veterinary World, 12(8): 1319-1326.
Abstract

Background and Aim: Ivermectin (IVM) has been used in veterinary practice to control different parasitic infestations over the past two decades. This study aimed to re-assess the acaricidal effects of IVM, as well as to evaluate its efficacy against Rhipicephalus (Boophilus) annulatus by determining the mortality rate, γ-aminobutyric acid (GABA) level, and oxidative/ antioxidative homeostasis (malondialdehyde [MDA] levels and glutathione S-transferase [GST] activities).

Materials and Methods: Adult females of Rhipicephalus (Boophilus) annulatus were picked from cattle farms in El-Beheira Governorate, Egypt. Ticks were equally allocated to seven experimental groups to assess the acaricidal potential of IVM chemotherapeutics in controlling R. (B.) annulatus. IVM was prepared at three concentrations (11.43, 17.14, and 34.28 μM of IVM).

Results: Mortality rate was calculated among the treated ticks. In addition, GABA, GST, and MDA biomarker levels were monitored. The data revealed a noticeable change in GST activity, a detoxification enzyme found in R. (B.) annulatus, through a critical elevation in mortality percentage.

Conclusion: IVM-induced potent acaricidal effects against R. (B.) annulatus by repressing GST activity for the initial 24 h after treatment. Collectively, this paper reports the efficacy of IVM in a field population of R. (B.) annulatus in Egypt.

Keywords: acaricidal, glutathione S-transferase, ivermectin, malondialdehyde, Rhipicephalus (Boophilus) annulatus, γ-aminobutyric acid.