Vet World Vol.12 December-2019 Article-24
Research Article
Veterinary World, 12(12): 2061-2069
https://doi.org/10.14202/vetworld.2019.2061-2069
Pasteurellaceae members with similar morphological patterns associated with respiratory manifestations in ducks
2. Department of Microbiology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.
3. Department of Poultry Diseases, Zagazig Provincial Laboratory, Animal Health Research Institute, Agricultural Research Centre, Sharkia, Egypt.
Background and Aim: A total of 112 freshly dead ducks aged from 2 to 20 weeks old with a history of respiratory manifestations were investigated for the implication of Pasteurellaceae family members.
Materials and Methods: Isolation and identification to the family level were conducted by conventional bacteriological methods, including microscopic examination and biochemical characterization. Identification to the species level was conducted by polymerase chain reaction (PCR) and analytical profile index (API) 20E kits.
Results: Conventional bacteriological isolation and biochemical characterization revealed the infection of 16/112 examined birds with a prevalence rate of 14.3%. PCR confirmed the detection of Pasteurellaceae family conserved genes RpoB and Bootz in 16/16 (100%) isolates. PCR was also used for genus and species identification of the isolated Pasteurellaceae members; the results revealed that 5/16 (31.3%) of isolates were Gallibacterium anatis and 2/16 of isolates (12.5%) were Pasteurella multocida. Riemerella anatipestifer, Mannheimia haemolytica, and Avibacterium paragallinarum were not detected by PCR. Biotyping by API 20E successfully identified 5/16 (31.3%) isolates that could not be typed by PCR and confirmed their belonging to Pasteurella pneumotropica. Neither the available PCR primer sets nor API 20E succeeded for species identification of 4/16 (25%) isolates. Antibiotic susceptibility profiling of isolates revealed that 16/16 (100%) of isolates demonstrated multidrug resistance (MDR) phenotypes. Moreover, 16/16 (100%) of isolates demonstrated a phenotypic resistance pattern to neomycin.
Conclusion: Combined genotypic, phenotypic, biotyping, and virulence characterizations are required for laboratory identification of pathogenic Pasteurellaceae. Moreover, P. multocida was not the prevailed member implicated in respiratory problems in ducks as P. pneumotropica, G. anatis, and unidentified strains were involved with higher prevalence. Chloramphenicol and ampicillin demonstrated the highest in vitro effects on the studied Pasteurellaceae. Furthermore, the prevalence of multidrug-resistant isolates signified the demand to implement targeted surveillance in the ducks' production sector, and MDR survey in poultry sectors in Egypt to apply effective control measures. Keywords: ducks, Gallibacterium anatis, Mannheimia haemolytica, Pasteurella multocida, Riemerella anatipestifer.
Keywords: ducks, Gallibacterium anatis, Mannheimia haemolytica, Pasteurella multocida, Riemerella anatipestifer.
How to cite this article: Eid S, Marouf S, Hefny HY, Al-Atfeehy NM (2019)
Received: 30-08-2019 Accepted: 25-11-2019 Published online: 26-12-2019
Corresponding author: Samah Eid E-mail: samaheid@ymail.com
DOI: 10.14202/vetworld.2019.2061-2069
Copyright: Eid, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
